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Preparation method and application deep sea water and fucoidin composite liquid

A technology of deep ocean water and fucoidan, applied in the field of marine biology, can solve the problems of insulin resistance and metabolic diseases that have not been reported, and achieve the effects of reducing glycogen output, regulating insulin signaling pathway, and strengthening the regulatory effect

Inactive Publication Date: 2018-12-18
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no report on the compounding of deep ocean water and fucoidan and its effects on insulin resistance and metabolic diseases

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  • Preparation method and application deep sea water and fucoidin composite liquid
  • Preparation method and application deep sea water and fucoidin composite liquid
  • Preparation method and application deep sea water and fucoidin composite liquid

Examples

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Effect test

Embodiment 1

[0039] Embodiment 1. Preparation method of deep ocean water and fucoidan compound solution

[0040] 1. The preparation method of deep ocean water with hardness of 1000ppm

[0041] Put the deep ocean water taken from 20km away from the coastline and 500m deep at the sea level into the water storage tank, filter it with a filter to remove phytoplankton and microorganisms in the seawater, and filter it through an activated carbon filter to further remove organic matter; the pretreatment The obtained deep ocean water enters the reverse osmosis device through a high-pressure pump to divide the deep ocean water into fresh water and concentrated water (the water quality of the fresh water is the same as that of pure water, or the water quality is close to pure water); the separated concentrated water is concentrated under reduced pressure, Concentrate its volume to 0.67-3.33% of the original volume, and then filter and remove the precipitated sodium chloride to obtain a highly concen...

Embodiment 2

[0046] Example 2. The improvement effect and mechanism of the compound solution of deep ocean water and fucoidan on insulin resistance

[0047] 1. Experimental method

[0048] 1) Cell culture: human liver cancer cells HepG2 cells, the cell culture medium is low-sugar DMEM (5.5mmol / L) medium containing 10% fetal bovine serum, the culture environment is 37 ° C, 5% CO2, every 3 days by 1:3 Ratio passaging once.

[0049] 2) Cytotoxicity of the compound solution of deep ocean water and fucoidan on HepG2 cells: collect the logarithmic phase cells, adjust the concentration of the cell suspension and inoculate them in a 96-well plate with 10,000 cells per well. 37°C, 5% CO 2 Culture, when the cell confluency reaches 70%, replace with serum-free DMEM, starve overnight, then add deep ocean water with a hardness of 1000ppm and different concentrations of fucoidan (5, 10, 50, 100, and 500mg / L) ) compound solution, 6 parallel wells were set up for each concentration. Continue culturing...

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Abstract

The invention provides a preparation method and application of deep sea water and fucoidin composite liquid. The composite liquid is prepared from deep sea water and fucoidin which are composited, wherein the hardness of the deep sea water is 1000 ppm, the molecular weight of the fucoidin is 270 KD, and the fucoidin is derived from brown alga sargassum pallidum. Experiments show that compared witha composited single component, the composite liquid disclosed by the invention can more obviously inhibit glycogen output quantity increment and lipid accumulation caused by high-concentration glucose induction, further can activate PI3K / Akt and AMPK signal channels to a greater extent and can improve insulin resistance; thus, the composite liquid has a more obvious intervention effect on metabolic diseases. The preparation method disclosed by the invention can provide theoretical support for the deep sea water and fucoidin composite liquid to research and develop health care products and drugs for preventing and treating the metabolic diseases and further can provide significance and enlightenment for high-valued utilization of marine resources of deep sea water, brown alga and the like.

Description

technical field [0001] The invention belongs to the technical field of marine biology, and relates to the comprehensive utilization of deep ocean water and brown algae resources, in particular to a method for preparing a compound solution of deep ocean water and fucoidan and its use in medicine or health care for the prevention and treatment of metabolic diseases. application in products. Background technique [0002] In recent years, with the changes in people's lifestyle and eating habits, the morbidity and mortality of metabolic diseases such as diabetes, hyperlipidemia, atherosclerosis, and obesity have gradually increased, which has had a serious impact on the health of the body. Develop relevant prevention and control strategies. Epidemiological studies have shown that insulin resistance (IR) is the common soil that causes endocrine and metabolic diseases such as diabetes, obesity, and hyperlipidemia, and it runs through the development of the disease, and is also an ...

Claims

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Application Information

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IPC IPC(8): A61K33/00A61P3/06A61P3/10A61P3/04A61P1/16A23L33/125A61K31/737
CPCA61K33/00A23L33/125A23V2002/00A61K31/737A61P1/16A61P3/04A61P3/06A61P3/10A61K2300/00A23V2200/30A23V2200/3262A23V2200/328A23V2200/332
Inventor 何珊彭维兵周洪雷付先军
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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