Method for separating and purifying plant sterols from seabuckthorn seeds

A technology for separation and purification of phytosterols, which is applied in the field of extraction of phytosterols, can solve the problems of high equipment cost, high vegetable oil dryness, and low production cost, and achieve the effects of shortened extraction time, simple operation method, and low equipment requirements

Pending Publication Date: 2018-12-21
QINGHAI UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The extraction of seabuckthorn oil is the pre-order step of seabuckthorn phytosterol extraction. At present, there are few extraction and purification methods for seabuckthorn oil from seabuckthorn seeds. There are four main extraction methods: (1) Squeeze method: all mechanical rolling, the obtained Oil can retain the original natural characteristics, but the oil yield is low, and it is mostly used for raw material pretreatment, not suitable for mass production
(2) Carbon dioxide extraction process: It is a production process adopted by large edible oil production enterprises in China. The advantage is that there is no solvent residue, and the disadvantage is that the equipment cost is high and the production cost is high. It is not suitable for the production of edible oil with a small output. , especially the production of raw material oils for health products such as seabuckthorn seed oil
(3) Solvent extraction process: It is a widely used vegetable oil extraction process. The advantages are low equipment cost, low production cost, and high oil yield. The disadvantage is that it is difficult to control solvent residues.
(4) Oil penetration and diffusion method: Vegetable oils such as sunflower oil are used as solvents to extract seabuckthorn seed oil, but the dryness of vegetable oils is much higher than that of organic solvents, the penetration and diffusion speed is low, and the extraction efficiency is poor

Method used

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  • Method for separating and purifying plant sterols from seabuckthorn seeds

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Experimental program
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Effect test

Embodiment 1

[0032] The selected sea buckthorn seeds are washed and aired. After crushing the sea buckthorn seeds, they are passed through a 60-mesh sieve, and dried in a constant temperature drying oven at 80°C for 5 hours to constant weight; the dried 100 g sea buckthorn seed powder is mixed with 800 mL n-hexane and soaked 120min, leaching with the aid of ultrasound at 60℃ for 40min, the extract is obtained by suction filtration, the filter residue is repeatedly extracted 3 times, and the extract is combined; the extract is concentrated under vacuum and reduced pressure to recover the solvent n-hexane solvent (recovery rate is about 80 %) to obtain sea buckthorn seed oil, the yield of sea buckthorn seed oil is 12.98%; after weighing 12.98 g of the sea buckthorn seed oil, add 5 times the volume concentration of 2.5 mol / L ethanol-potassium hydroxide solution, and The saponification liquid was obtained in a water bath at ℃ for 3 hours; after cooling 60 mL of the obtained saponification liquid...

Embodiment 2

[0034] The selected sea buckthorn seeds are washed and aired. After crushing the sea buckthorn seeds, they are passed through a 50-mesh sieve, and they are dried in a constant temperature drying oven at 75°C for 4.5 hours to constant weight; after mixing 100 g of dried sea buckthorn seed powder with 600 mL of n-hexane Soak for 80min, leaching with the aid of ultrasound at 50℃ for 30min. The extract is obtained by suction filtration. The filter residue is extracted 3 times and combined the extract; the extract is concentrated under vacuum and reduced pressure to recover the solvent n-hexane solvent (recovery rate is about 80%) to obtain sea buckthorn seed oil, the yield of sea buckthorn seed oil is 12.91%; after weighing 12.91 g of sea buckthorn seed oil, add 3 times the volume concentration of 2.0 mol / L ethanol-potassium hydroxide solution 30 After cooling, take 40mL of the obtained saponification solution, add 2 times volume of distilled water, 80mL, add saponification solution...

Embodiment 3

[0036] The selected sea buckthorn seeds are washed and aired. After crushing the sea buckthorn seeds, they are passed through a 40-mesh sieve, and dried in a constant temperature drying oven at 70°C for 4 hours to constant weight; the dried 100 g sea buckthorn seed powder is mixed with 400 ml n-hexane and soaked for 30 minutes , Leaching for 20 minutes with the aid of ultrasonic at 40℃, extracting and filtering to obtain the extract, the filter residue is repeatedly extracted 3 times, and the extract is combined; the extract is concentrated under vacuum and reduced pressure to recover the solvent n-hexane solvent (recovery rate is about 80% ) To obtain sea buckthorn seed oil, the yield of sea buckthorn seed oil is 11.41%; 11.41 g of sea buckthorn seed oil is weighed, add 2 times the volume concentration of 1.5 mol / L ethanol-potassium hydroxide solution 20mL, and The saponification liquid was obtained in a water bath at ℃ for 1.5 hours; after cooling 20 mL of the obtained saponif...

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Abstract

The invention relates to the technical field of extracting plant sterols, in particular to a method for separating and purifying plant sterols from seabuckthorn seeds. The method specifically comprises the steps of seabuckthorn seed smashing and drying, ultrasonic-assisted leaching and suction filtration, vacuum concentration, saponification, cooling, extraction, water washing, concentration, water bath reflux, recrystallization, washing, centrifugation and drying. Compared with the prior art, the method for separating and purifying the plant sterols from the seabuckthorn seeds has the advantages of simple operation method, low equipment requirement, low cost of reagents, low energy consumption, and high extraction rate of seabuckthorn seed oil, and the highest extraction rate can reach to12.98%, seabuckthorn sterol has a high purity of up to 1.487%, and the method for separating and purifying the plant sterols from the seabuckthorn seeds is suitable for use in small and medium-sizedproduction and processing enterprises in laboratories or production.

Description

Technical field [0001] The invention relates to the technical field of extraction of plant sterols, in particular to a method for separating and purifying plant sterols from seabuckthorn seeds. Background technique [0002] Sea buckthorn ( Hippophae rhamnoides Linn. ) Is a shrub or small tree of the genus Elaeagnus, also known as vinegar, black thorn, acid thorn, Qicha Rigana (Mongolian name), Dap (Tibetan name), Jihan (Uyghur name). Mainly distributed in the vast areas of Eurasia, it has the characteristics of cold and drought resistance, salt and alkali resistance, barren tolerance, and wide ecological adaptability. As a "pioneer tree species for ecological protection" for windbreak and sand fixation and soil and water conservation, it is planted in large areas in my country. The fruit of the sea buckthorn tree is called sea buckthorn fruit, sea buckthorn juice can be used as a beverage, sea buckthorn pulp can be separated from the fruit oil, and sea buckthorn seeds can be use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C11B3/00
CPCC11B1/04C11B1/106C11B3/001
Inventor 张得钧张本印薛延团王树林张晓凤杨永晶周怡
Owner QINGHAI UNIVERSITY
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