Method for detecting microorganisms by virtue of liquid phase chip technology
A technical detection and liquid phase chip technology, applied in the biological field, can solve the problems of inability to detect a variety of microorganisms
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] According to a kind of liquid phase chip technology detection microorganism method provided by the present invention, comprises the following steps:
[0027] S1, according to the gene sequence of Brucella (Bru) in Genabank, use the Primer 5.0 sequence analysis software for gene sequence analysis, design and synthesize the nucleotide shown in SEQ ID NO.1 with an amino group modified at the 5' end The brucella probe of sequence, as shown in table 1;
[0028] According to the gene sequence of Salmonella (Sal) in Genabank, the Primer 5.0 sequence analysis software was used for gene sequence analysis, and the Salmonella probe with the nucleotide sequence shown in SEQ ID NO.2 with amino groups modified at the 5' end was designed and synthesized. ,As shown in Table 1;
[0029] According to the gene sequence of rabies virus (Rab) in Genabank, use Primer 5.0 sequence analysis software to carry out gene sequence analysis, design and synthesize the rabies virus with the nucleotid...
Embodiment 2
[0045] The specific method for obtaining the PCR product to be tested in Example 1 also includes:
[0046] SS1, Brucella primers are designed upstream and downstream of the Brucella probe, including the Brucella upstream primer with the nucleotide sequence shown in SEQ ID NO.7 modified with biotin at the 5' end and with The Brucella downstream primer of the nucleotide sequence shown in SEQ ID NO.8, as shown in table 2.
[0047] Salmonella primers are designed on the upstream and downstream of the Salmonella probe, including the Salmonella upstream primer with the nucleotide sequence shown in SEQ ID NO.9 and the nucleotide shown in SEQ ID NO.10 with the 5' end modified with biotin The sequence of the downstream primers for Salmonella is shown in Table 2.
[0048] Rabies virus primers are designed upstream and downstream of the rabies virus probe, including the rabies virus upstream primer with the nucleotide sequence shown in SEQ ID NO.11 modified with biotin at the 5' end and...
Embodiment 3
[0059] Respectively utilize Brucella primers, Salmonella primers, rabies virus primers, Toxoplasma gondii primers, Pasteurella primers and Campylobacter primers to amplify the PCR products obtained respectively and simultaneously amplify the multiple PCR products obtained in Example 2 The specificity of the product was tested by electrophoresis.
[0060] The results of electrophoresis were as figure 1 Shown, wherein No. 1-6 swimming lane is the electrophoresis figure that utilizes Brucella primers, Salmonella primers, rabies virus primers, Toxoplasma gondii primers, Pasteurella primers and Campylobacter primers to amplify the gene BA of the whole body of Pasteurella; Lane 7 is the electrophoresis image of simultaneous amplification of the gene BA of the whole body of Pasteurella using primers for Brucella, Salmonella, rabies, Toxoplasma, Pasteurella and Campylobacter; The primers and the multiple primers including Brucella primers, Salmonella primers, rabies virus primers, To...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More