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Method for extracting ginkgo leaf flavonoids

An extraction method and technology of lutein, applied in the field of extraction of ginkgo leaf flavonoids, can solve the problems of low extraction rate and low cost

Inactive Publication Date: 2018-12-28
江苏普惠银杏产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction method of ginkgo leaf flavonoids is currently researched with water extraction method, which is low in cost but low in extraction rate; among organic solvent extraction methods, ethanol extraction is highly efficient and non-toxic, and is currently the most used method; Han Yuqian et al. Using supercritical fluid extraction, reflux in 70% ethanol solution and CO 2 The active ingredients in Ginkgo biloba were extracted by supercritical fluid extraction, and the recovery rate of ginkgo flavonoids was 84.4%, which was more than twice the recovery rate of conventional extraction methods; the extraction rate of flavonoids by ethanol ultrasonic leaching method could reach 86.7%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for extracting ginkgo leaf flavonoids, comprising the steps of:

[0019] S1, get 100Kg of Ginkgo biloba leaves, pulverize, add 200 Kg of water, 3Kg of sodium chloride, 2Kg of hydrochloric acid, 3Kg of chitosan, and 3 Kg of bromelain, carry out enzymolysis, and then inactivate the enzyme to obtain the enzymolysis solution;

[0020] S2. After adjusting the pH of the enzymolysis solution to 5, extract with petroleum ether, and take the organic phase;

[0021] S3, adding an equal weight of 3wt.% polyethylene glycol in a low-concentration aqueous solution to the organic phase, stirring evenly, performing extraction, and taking the aqueous phase;

[0022] S4. Send the water phase to the ultrafiltration membrane for filtration, then concentrate the permeate of the ultrafiltration membrane with a forward osmosis membrane, and then dry it to obtain ginkgo biloba flavonoids.

[0023] The particle size of the crushed ginkgo leaves in S1 is 20 mesh. The temperature of en...

Embodiment 2

[0025] A method for extracting ginkgo leaf flavonoids, comprising the steps of:

[0026] S1, take 150Kg of ginkgo leaves, pulverize, add 400 Kg of water, 3Kg of sodium chloride, 2Kg of hydrochloric acid, 3Kg of chitosan, and 5 Kg of bromelain, carry out enzymolysis for 1 hour, and then inactivate the enzyme to obtain the enzymolysis solution;

[0027] S2. After adjusting the pH of the enzymolysis solution to 6, extract with petroleum ether, and take the organic phase;

[0028] S3, adding an equal weight of 3wt.% polyethylene glycol in a low-concentration aqueous solution to the organic phase, stirring evenly, performing extraction, and taking the water phase;

[0029] S4. Send the water phase to the ultrafiltration membrane for filtration, then concentrate the permeate of the ultrafiltration membrane with a forward osmosis membrane, and then dry it to obtain ginkgo biloba flavonoids.

[0030] The particle size of the crushed ginkgo leaves in S1 is 40 mesh. The temperature of...

Embodiment 3

[0032] A method for extracting ginkgo leaf flavonoids, comprising the steps of:

[0033] S1, take 120Kg of ginkgo leaves, pulverize, add 300Kg of water, 3Kg of sodium chloride, 2Kg of hydrochloric acid, 3Kg of chitosan, and 4Kg of bromelain, perform enzymolysis for 1 hour, and then inactivate the enzyme to obtain the enzymolysis solution;

[0034] S2. After adjusting the pH of the enzymolysis solution to 5, extract with petroleum ether, and take the organic phase;

[0035] S3, adding an equal weight of 2wt.% polyethylene glycol in a low-concentration aqueous solution to the organic phase, stirring evenly, performing extraction, and taking the aqueous phase;

[0036] S4. Send the water phase to the ultrafiltration membrane for filtration, then concentrate the permeate of the ultrafiltration membrane with a forward osmosis membrane, and then dry it to obtain ginkgo biloba flavonoids.

[0037] The particle size of the crushed ginkgo leaves in S1 is 30 mesh. The temperature of e...

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PUM

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Abstract

The invention relates to a method for extracting ginkgo leaf flavonoids, and belongs to the technical field of natural production extraction. The method comprises the following steps: taking ginkgo leaves, crushing, adding water and bromelain, carrying out enzymolysis, and carrying out enzyme deactivation to obtain enzymatic hydrolysate; extracting the enzymatic hydrolysate with petroleum ether, and collecting an organic phase; adding the organic phase into a low-concentration aqueous solution of polyethylene glycol of an equal weight, uniformly stirring, extracting, and collecting a water phase; feeding the water phase to an ultrafiltration membrane, filtering, then concentrating penetrating fluid of the ultrafiltration membrane by using a positive penetration membrane, and drying to obtain the ginkgo leaf flavonoids. According to the invention, after enzymolysis of the ginkgo leaves, flavonoids can be effectively released.

Description

technical field [0001] The invention relates to a method for extracting ginkgo leaf flavonoids, belonging to the technical field of natural product extraction. Background technique [0002] Flavonoids are a class of compounds that exist in nature and have the structure of 2-phenylchromanone. There is a ketone carbonyl group in their molecules, and the oxygen atom at the first position is basic and can form a salt with strong acid. Most of its hydroxyl derivatives are yellow, so they are also called flavonoids or flavonoids. Flavonoids are usually combined with sugars in plants to form glycosides, and a small part exists in the form of free states (glycones). Most plants contain flavonoids, which play an important role in plant growth, development, flowering, fruiting, antibacterial and disease prevention. [0003] Except for a few free flavonoids, most of them combine with sugar to form glycosides. The sugar group is mostly connected at the C8 or C6 position, and the conn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/19A61K2236/35A61K2236/51A61K2236/53
Inventor 常亚
Owner 江苏普惠银杏产业发展有限公司