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Separation and purification method of xylobiose and xylotriose and application thereof

A technology for separation and purification of xylotriose, applied in chemical instruments and methods, disaccharides, applications, etc.

Inactive Publication Date: 2019-01-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No studies have reported the effects of xylobiose and xylotriose on chicken gut bacteria

Method used

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  • Separation and purification method of xylobiose and xylotriose and application thereof
  • Separation and purification method of xylobiose and xylotriose and application thereof
  • Separation and purification method of xylobiose and xylotriose and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Coarse separation of xylobiose and xylotriose in embodiment 1 xylooligosaccharides

[0046] Prepare xylo-oligosaccharide powder into 500g / L xylo-oligosaccharide solution, centrifuge at 4000r / min for 10min at high speed, take its supernatant (repeat several times until the supernatant is clear and transparent), and adjust the pH to 6-8. Store the processed xylo-oligosaccharide solution in a refrigerator at 4°C until use. A protein liquid chromatography system (AKTA purifier system, GE Company) was used for crude separation of single xylo-oligosaccharide components. The chromatographic column is 60×2.5cm I.D., the chromatographic medium is polyacrylamide gel Bio-Gel P-2, eluted with degassed ultrapure water, the elution rate is 60mL / h, the column temperature is 35°C, and the eluent Collect 6 mL per tube and collect 95 tubes.

[0047] 1. Determination of single component reducing sugar concentration

[0048] Use the DNS (3,5-dinitrosalicylic acid) method to measure the ...

Embodiment 2

[0060] Secondary separation of xylobiose and xylotriose in xylooligosaccharides in embodiment 2

[0061] The final xylobiose and xylotriose liquids obtained in Example 1 are subjected to secondary separation respectively, and the secondary separation adopts a constant flow pump (HUXI-Pump) and an automatic collector (BSZ-100); the chromatographic column is 100× 1.2cm I.D., the chromatographic medium is polyacrylamide gel Bio-Gel P-2, eluted with degassed ultrapure water, the elution rate is 12mL / h, the eluent is collected in 1.5mL per tube, and 95 tubes are collected. The determination method and steps are the same as step 1, and the concentration of reducing sugar in the collection tube is measured by DNS method, and the secondary separation and elution curve is drawn.

[0062] 1. Determination of single component reducing sugar concentration

[0063] The results of reducing sugar concentration measurement after the secondary separation of xylobiose are as follows: Figure ...

Embodiment 3

[0066] Example 3 Effect of xylobiose and xylotriose on chicken intestinal bacteria

[0067] 1. Experimental design

[0068] Thirty healthy AA chicks with similar body weight at 1 day were selected and randomly divided into 3 treatment groups, each treatment group had 5 replicates, and each replicate had 2 chickens. The three treatment groups were control group, xylobiose group and xylotriose group. All the chicks in the experiment ate the basic diet and were treated by gavage. The concentration of monosaccharide in the treatment group was 6.0g / L, and the perfusion meter was 0.5mL, once a day at 9 o'clock in the morning, for 7 consecutive days. The test broilers were kept in the same chicken house, with ad libitum feeding and drinking, 24h continuous light, immunization procedures and other management were carried out as usual.

[0069] 2. Sample collection

[0070]When the chicks were reared on the 8th day of age, all the chicks in each treatment group were slaughtered, dis...

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Abstract

The invention discloses a separation and purification method of xylobiose and xylotriose and application thereof. The separation and purification method comprises the following steps (1) crude separation is carried out on xylooligosaccharide through Bio-Gel P-2, and a crude separation elution curve is drawn; (2) referring to crude separation elution curve results in step (1), secondary separationis carried out through Bio-Gel P-2 to obtain the xylobiose and the xylotriose after secondary separation; (3) purity testing is carried out on the xylobiose and the xylotriose by adopting a high efficiency liquid chromatography; and finally, the purity of the xylobiose and the purity of the xylotriose can reach 97.05% and 97.85% respectively. The xylobiose and xylotriose obtained by the separationand purification method can significantly increase the number of lactobacillus florae of the ileum and cecum of chicken; the xylotriose significantly reduces the number of florae of escherichia coliand salmonella in the cecum of the chicken, and an application prospect is good in improving the intestinal health of the chicken.

Description

technical field [0001] The invention belongs to the technical field of functional oligosaccharide production, and in particular relates to a method for separating and purifying xylobiose and xylotriose and its application. Background technique [0002] Since the discovery and use of antibiotics, their role has been indispensable. Antibiotics can prevent animal diarrhea and promote animal growth by inhibiting the subclinical symptoms produced by antibiotics in the environment, but at the same time, they can also cause drug residues, bacterial resistance, etc. Negative problems, causing serious harm to animals, people and the ecological environment. However, after antibiotics are banned in feed, animal production performance is significantly reduced, and the mortality rate is significantly increased, which seriously affects the healthy development of animal husbandry. Therefore, it is imminent to develop new high-safety and green alternative feed antibiotics. As a new type o...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K20/163C07H3/04C07H3/06C07H1/06
CPCA23K20/163A23K50/75C07H1/06C07H3/04C07H3/06
Inventor 左建军冯定远马岩余亲平高永峰叶慧曹庆云欧阳海燕
Owner SOUTH CHINA AGRI UNIV
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