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Preparation method of mesoporous silicon dioxide nanomaterial with fluorescent imaging and drug loading functions

A technology of mesoporous silica and fluorescence imaging, which is applied in the direction of silica, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of complex preparation methods and achieve the effect of simple operation

Inactive Publication Date: 2019-01-04
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings of the existing complex preparation methods of fluorescent silica nanomaterials, the present invention provides a preparation method of mesoporous silica nanomaterials with dual functions of fluorescence imaging and drug loading

Method used

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  • Preparation method of mesoporous silicon dioxide nanomaterial with fluorescent imaging and drug loading functions
  • Preparation method of mesoporous silicon dioxide nanomaterial with fluorescent imaging and drug loading functions
  • Preparation method of mesoporous silicon dioxide nanomaterial with fluorescent imaging and drug loading functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1, preparation of mesoporous silica nanoparticles.

[0025] Weigh 250 mg of cetyltrimethylammonium bromide and dissolve it in 120 mL of deionized water, then add 0.87 mL of 1.0 mol / L sodium hydroxide solution, and stir vigorously at 60°C for 20 min. After cetyltrimethylammonium bromide was completely dissolved, 1.0 mL of ethyl orthosilicate was slowly added, and stirring was continued for 1 hour. After the reaction, the product was cooled to room temperature, centrifuged, and washed three times with deionized water and ethanol. Finally, the ethanol solution containing ammonium nitrate with a mass volume ratio of 5 g / L was refluxed for 12 hours to remove the template agent, washed three times with deionized water and ethanol in turn, and dried in vacuum at 60 °C to constant weight to obtain mesoporous silica. Nanoparticles, codenamed MSN.

[0026] Step 2: Amination modification is performed on the surface of the mesoporous silica nanoparticles.

[0027] Weigh 100...

Embodiment 2

[0033]Step 1, preparation of mesoporous silica nanoparticles.

[0034] Weigh 300mg of cetyltrimethylammonium bromide and dissolve it in 120mL of deionized water, then add 0.98mL of 1.5mol / L sodium hydroxide solution, and stir vigorously at 70°C for 25min. After cetyltrimethylammonium bromide was completely dissolved, 1.5 mL of ethyl orthosilicate was slowly added, and stirring was continued for 1.5 hours. After the reaction, the product was cooled to room temperature, centrifuged, and washed four times with deionized water and ethanol. Finally, the ethanol solution containing ammonium nitrate with a mass volume ratio of 6 g / L was refluxed for 18 hours to remove the template agent, washed with deionized water and absolute ethanol for 4 times, and vacuum-dried at 70 °C to constant weight to obtain the mesoporous Silicon oxide nanoparticles, code-named MSN.

[0035] Step 2: Amination modification is performed on the surface of the mesoporous silica nanoparticles.

[0036] Weig...

Embodiment 3

[0041] Step 1, preparation of mesoporous silica nanoparticles.

[0042] Weigh 350mg of cetyltrimethylammonium bromide and dissolve it in 200mL of deionized water, then add 1.85mL of 2.0mol / L sodium hydroxide solution, and stir vigorously at 80°C for 30min. After cetyltrimethylammonium bromide was completely dissolved, 3.0 mL of ethyl orthosilicate was slowly added, and stirring was continued for 2 hours. After the reaction, the product was cooled to room temperature, centrifuged, and washed five times with deionized water and ethanol in sequence. Finally, the ethanol solution containing ammonium nitrate with a mass volume ratio of 7 g / L was refluxed for 24 hours to remove the template agent, washed with deionized water and absolute ethanol for 5 times, and vacuum-dried at 80 °C to constant weight to obtain the mesoporous bismuth Silicon oxide nanoparticles, code-named MSN.

[0043] Step 2: Amination modification is performed on the surface of the mesoporous silica nanopartic...

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Abstract

The invention discloses a preparation method of a mesoporous silicon dioxide nanomaterial with fluorescent imaging and drug loading functions. The preparation method is used for solving the technicalproblem that the conventional preparation method of the fluorescent silicon dioxide nanomaterial is complicated. The method comprises the steps of preparing a mesoporous silicon dioxide nano particleby taking cetyl trimethyl ammonium bromide as a template and ethyl orthosilicate as a silicon source by a sol gel method, taking 3-aminopropyl triethoxy alkyl as an amination modification reagent to form aminated mesoporous silicon dioxide, allowing chloro fluorescein carboxyfluorescein diacetate succinimidyl ester to be in reaction with aminated mesoporous silicon dioxide to form a chloro fluorescein-labeled mesoporous silicon dioxide nano particle. The drug loading and fluorescent imaging functions are achieved by firmly linking a fluorescent dye onto mesoporous silicon dioxide by an amido bond. The method is simple to operate, and a strongly corrosive reagent is not required.

Description

technical field [0001] The invention relates to a preparation method of a mesoporous silicon dioxide nanometer material, in particular to a preparation method of a mesoporous silicon dioxide nanometer material with dual functions of fluorescence imaging and drug loading. Background technique [0002] Mesoporous silica nanoparticles (MSN) is a new type of inorganic polymer. The advantages of mesoporous silica nanoparticles include: (1) It has a huge specific surface area (>900m 2 / g) and pore volume (>0.9cm 3 / g), adjustable pore size, large drug loading capacity, high encapsulation efficiency, and can effectively avoid drug diffusion; (2) MSN can enter cells through endocytosis to avoid being captured by lysosomes; (3) good Biocompatibility; (4) By modifying its surface with functional groups, the functions of active targeting, drug controllable and sustained release can be realized. Therefore, it has gradually become a carrier for drug delivery. [0003] Malignant ...

Claims

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Application Information

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IPC IPC(8): C01B33/18B82Y40/00C09K11/59A61K49/00A61K47/69A61K31/428A61P35/00
CPCA61K31/428A61K49/0021A61K49/005A61K49/0093A61K47/6949A61P35/00B82Y40/00C01B33/18C01P2002/72C01P2004/62C01P2004/64C01P2006/14C01P2006/17C09K11/59
Inventor 武祥龙张飞刘浩陈强董凯卢婷利
Owner NORTHWESTERN POLYTECHNICAL UNIV
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