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Application of self-assembling oligopeptide to rapid repair of skin tissue trauma

A technology of self-assembling short peptides and tissue trauma, which is applied in the field of nanobiological regenerative medicine, can solve the problems of poor curative effect and slow repair of skin tissue damage, and achieve the effect of less inflammation, faster wound healing and better repair quality

Pending Publication Date: 2019-01-04
罗忠礼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides the application of a self-assembled short peptide for rapid repair of skin tissue wounds to solve the technical problems of slow repair of skin tissue damage and poor curative effect in existing medicine

Method used

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  • Application of self-assembling oligopeptide to rapid repair of skin tissue trauma
  • Application of self-assembling oligopeptide to rapid repair of skin tissue trauma
  • Application of self-assembling oligopeptide to rapid repair of skin tissue trauma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Example 1: Atomic force microscopy (AFM) of self-assembled short peptide Sciobio-II self-assembled for 24h

[0039] The self-assembled short peptide after self-assembly at 37°C for 24 hours was shown in the atomic force microscope. After 24 hours of assembly, the short peptide formed a dense nanorod-like fiber structure, and the rod-like nanofibers were assembled into a dense nanofiber network scaffold. , to provide support and adhesion for the three-dimensional culture of cells ( figure 1 ).

Embodiment 2

[0040] Embodiment 2: The cultivation of HFF-1 cells in the three-dimensional environment constructed by Sciobio-II

[0041] Human skin fibroblasts (HFF-1) were routinely cultured in a two-dimensional environment, high-glucose DMEM medium with 15% FBS and 1% double antibody, CO 2 Culture in a cell culture incubator (37°C, 5%). The three-dimensional culture steps are as follows: (1) Cells are adhered to grow to about 80% for three-dimensional culture; (2) Wash the cells twice with PBS before digestion with trypsin (0.25%); (3) Centrifuge at 1000rpm and count; (4) Add short Peptide and other solutions, mix well to form a three-dimensional suspension cell solution. Place the 96-well plate in a constant temperature incubator (37°C, 5% CO 2 ) for cultivation, observation and analysis. In a two-dimensional environment, HFF-1 is in a state of adherent growth, showing a long fusiform shape. When cultured in the three-dimensional environment constructed by Sciobio-II, the cells are ...

Embodiment 3

[0042] Example 3: Acridine orange / ethidium bromide staining of HFF-1 cells in the three-dimensional environment constructed by Sciobio-II

[0043] The steps are as follows: (1) HFF-1 cells were cultured in the two-dimensional environment and the three-dimensional environment constructed by Sciobio-II. About 2000 cells were seeded per well. The well plate was placed at 37°C, 5% CO 2 cultured in a constant temperature incubator. 24h after plating was recorded as the first day, and AO / EB staining was carried out on the 1st, 3rd, 5th and 7th day; (2) AO dyeing solution and EB dyeing solution were mixed evenly in the dark to make AO / EB dyeing solution; ( 3) Take the well plate and discard the culture medium. Wash with PBS. Fix with 20 μl / well of 4% paraformaldehyde for 10-15 minutes. Add 15 μl / well of AO / EB staining solution in the dark, and stain for 10 minutes. Use PBS to wash away the background. Observed and photographed under a fluorescent inverted microscope.

[0044]...

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Abstract

The invention discloses application of self-assembling oligopeptide to rapid repair of skin tissue trauma, and belongs to the field of nano-biological regenerative medicine. The amino acid sequence ofthe self-assembling oligopeptide is Arg Leu Glu Cys Lys Ala Asp Ala Arg Leu Glu Val Lys Ala Asp Ala-NH2. A new application field is widen for the existing self-assembling oligopeptide, the use rangetype of the self-assembling oligopeptide is expanded, the research and development value of the self-assembling oligopeptide is increased, a feasible research direction is provided for repair of skintissue injury, and the field of tissue repair is widened.

Description

technical field [0001] The invention belongs to the field of nanobiological regenerative medicine, and in particular relates to the application of a self-assembled short peptide in rapid repair of skin tissue wounds. Background technique [0002] As the largest organ of the human body and the first line of defense of the human body, the skin has important functions in the course of life. Such as protecting, hydrating, isolating chemical substances, viruses and pathogens, participating in the excretion of the human body, regulating body temperature, and feeling external stimuli. [0003] Skin trauma not only affects normal life and appearance, but will be life-threatening in severe cases. The essence of skin wound healing is to isolate the source of infection and repair tissue. Skin wound repair is a complex process mainly manifested by non-specific forms of wound healing through fibrosis and scarring. Its recovery time is divided into multiple stages, and each stage inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61K38/10A61P17/02
CPCA61K38/10A61P17/02C12N5/0062C12N2513/00
Inventor 张慧楠文静兰世建吴书祎黄岚罗忠礼张宇
Owner 罗忠礼
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