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Cell preservation solution and use thereof

A technology for preserving liquids and cells, applied in the field of biology, to achieve strong acid-base buffering capacity, avoid cell damage, and simple cell transportation

Inactive Publication Date: 2019-01-11
苏州瑞徕生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this study, the cell density in the preservation solution was 1*10 5 Cells / mL works best, but in many cases, for the convenience of storing and transporting a large number of cells, the cell density is high, so it is necessary to develop a preservation solution suitable for room temperature transport with better effect

Method used

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  • Cell preservation solution and use thereof
  • Cell preservation solution and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The preparation method of embodiment 1 cell preservation solution

[0030] The cell preservation solution used in the following examples uses the same preparation method, specifically as follows:

[0031] Add electrolytes to 900ml of deionized water, stir and dissolve on a magnetic stirrer, then add various amino acids, stir and dissolve, add HEPES, glucose, trehalose, L sodium ascorbate and antioxidants, fully dissolve, add water to 1L, Adjust the pH to 7.2-7.5. Sterilize by filtration using a disposable cup filter with a 0.22 μm filter membrane (Thermo Scientific, Cat. No. 5954520).

Embodiment 2

[0032] Example 2 Cell Preservation Solution 1

[0033] Cell preservation solution 1, containing electrolytes, amino acids, 5mM hydroxyethylpiperazineethanesulfonic acid (HEPES), 5.5mM glucose, 0.01M trehalose, 50mg / L L sodium ascorbate, and 50μM MnTBAP;

[0034] The electrolyte components are: NaCl 6g / L, KCl 0.2g / L, NaCl 2 HPO 4 0.06g / L, NaH 2 PO 4 0.04g / L, MgCl 2 ·6H 2 O 0.1g / L, MgSO 4 0.04g / L, CaCl 2 0.1g / L, CuSO 4 ·5H 2 O 0.001mg / L, ZnSO 4 ·7H 2 O0.33mg / L, NaHCO 3 1.2g / L.

[0035] The amino acid composition is: L-alanine 0.1g / L, L-arginine 0.4g / L, L-asparagine 0.2g / L, L-cysteine ​​0.1g / L, L-glutamine Aminoamide 0.2g / L, Glycine 0.1g / L, L-Histidine 0.2g / L, L-Isoleucine 0.1g / L, L-Leucine 0.1g / L, L-Methionine 0.05g / L L, L-phenylalanine 0.1g / L, L-serine 0.1g / L, L-threonine 0.2g / L, L-tryptophan 0.01g / L, L-tyrosine 0.2g / L , L-valine 0.05g / L.

Embodiment 3

[0036] Example 3 Cell Preservation Solution 2

[0037] Cell preservation solution 2, containing electrolytes, amino acids, 20mM hydroxyethylpiperazineethanesulfonic acid (HEPES), 24.5mM glucose, 0.05M trehalose, 100mg / L L sodium ascorbate, and 10μM MnTMPyP;

[0038] The electrolyte components are: NaCl 8g / L, KCl 0.4g / L, NaCl 2 HPO 4 0.08g / L, NaH 2 PO 4 0.06g / L, MgCl 2 ·6H 2 O 0.3g / L, MgSO 4 0.06g / L, CaCl 2 0.13g / L, CuSO 4 ·5H 2 O 0.002mg / L, ZnSO 4 ·7H 2 O0.53mg / L, NaHCO 3 2.8g / L.

[0039] The amino acid composition is: L-alanine 0.3g / L, L-arginine 0.6g / L, L-asparagine 0.3g / L, L-cysteine ​​0.14g / L, L-glutamine Aminoamide 0.8g / L, Glycine 0.3g / L, L-Histidine 0.3g / L, L-Isoleucine 0.15g / L, L-Leucine 0.15g / L, L-Methionine 0.1g / L L, L-phenylalanine 0.15g / L, L-serine 0.3g / L, L-threonine 0.4g / L, L-tryptophan 0.03g / L, L-tyrosine 0.4g / L , L-valine 0.15g / L.

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Abstract

The invention discloses a cell preservation solution and a use thereof. The preservation solution comprises hydroxyethylpiperazineethanesulfonic acid, sodium hydrogencarbonate, glucose, trehalose, sodium L ascorbate and one or more antioxidants (such as MnTBAP, MnTMPyP, L-reduced glutathione and N-acetyl-L-cysteine) in a solution containing an electrolyte and amino acids. The cell preservation solution provides nutrients and metabolic energy for cells, contains various pH buffers and radical scavengers, can retain cell activity and functions at 2-8 DEG C or normal temperature, facilitates cellpreservation and transport and solves the problem that the cells need long function recovery time after traditional transport.

Description

technical field [0001] The invention relates to cell preservation and transportation technology, in particular to a cell preservation solution and its application, belonging to the technical field of biology. Background technique [0002] With the rapid development of cell therapy technology in recent years, more and more evidence shows that cell therapy has important application prospects in the treatment of various diseases. Cell therapy refers to using the characteristics of certain cells with specific functions, using bioengineering methods to obtain and / or through in vitro expansion, special culture, etc., to make these cells have the ability to enhance immunity, kill pathogens and tumor cells, and promote tissue regeneration. Therapeutic effects such as organ regeneration and body rehabilitation, so as to achieve the purpose of treating diseases. [0003] Since various cell therapy techniques require appropriate treatment of target cells in vitro, these operations are...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 娄晋宁张文健
Owner 苏州瑞徕生物科技有限公司
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