Method for preparing collagen hydrolysate products by using enzymatic method

Abstract

The invention discloses a method for preparing collagen hydrolysate product by an enzymatic method, which comprises the following steps: preparing collagen powder with pure water into a mass fractionof 10-20% collagen aqueous solution, pH 7-7.5 and adding compound enzyme, heating in water bath and stirring for enzymatic hydrolysis, heating and killing enzyme aft that reaction is finished, coolingto room temperature, centrifuging and filtering to obtain collagen hydrolysate, and spraying and drying to obtain powder. The invention adopts the hydrolysis method to break the polypeptide bond of the collagen, so that the macromolecular collagen which is poor in solubility and difficult to be absorbed by the organism is converted into the small molecular polypeptide which is good in water solubility and easy to be absorbed by the organism, and the absorption rate of the collagen is greatly improved. Enzymatic hydrolysis of collagen peptides with complex enzymes has a good hydrolysis effect,and the molecular weight of collagen peptides is clear, the content of collagen protein after hydrolysis is high, the molecular weight of collagen peptides is less than 1000, the hydrolysis is complete, and the effect is good.

Description

technical field [0001] The invention relates to the field of polypeptide applications, in particular to a method for enzymatically preparing collagen hydrolyzate. Background technique [0002] Collagen is a kind of biological polymer, which is the main component of animal connective tissue. It is similar to the collagen structure of human skin. The molecule is rich in amino acids and hydrophilic groups. It has certain surface activity and good compatibility. It has a moisturizing effect, and can inhibit the conversion of tyrosine in skin tissue into melanin. It has the functions of moisturizing, whitening, anti-wrinkle, and freckle removal. It is mostly used in skin care products and health food. [0003] Collagen molecule is a triple helical structure formed by three α-peptide chains, with a relative molecular mass of about 290kDa. It is difficult to be absorbed and utilized after entering the human body. Scientific research has proved that the human body’s absorption rate ...

AI Technical Summary

Problems solved by technology

The chemical degradation method uses acid and alkali to hydrolyze collagen, and 0.5mol / L acetic acid, citric acid or hydrochloric acid can be used to treat collagen at pH 2-3, but acid hydrolysis has a low hydrolysis rate of collagen and is not specific , it is difficult to control the degree of hydrolysis during the hydrolysis process; the enzymatic degradation method will not destroy the configuration of amino acids

Patent No. CN 107142294 A discloses a method for preparing collagen peptide products from buckskin, and Patent No. CN 107177658 A discloses a method for preparing cartilage collagen peptides. Although the above two methods enzymatically hydrolyze collagen, the The enzymatic hydrolysis is not complete and cannot be fully absorbed by the human body