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Method for rapidly screening small experimental fish to construct pancreatic fat infiltration model

A technology of fat infiltration and modeling, which is applied in fish farming, climate change adaptation, animal feed, etc. It can solve the problems of increased animal mortality, high maintenance costs, and decreased success rate of modeling, and achieve clear liver structure and liver body. The effect of large ratio and easy operation of materials

Active Publication Date: 2019-01-22
GUANGDONG LAB ANIMALS MONITORING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high cost of construction and maintenance of the large / mouse model, the consumption of feed and drugs is large, and it is not suitable for high-throughput experiments; at the same time, large / mouse models under toxic damage such as intraperitoneal injection of carbon tetrachloride are used The model will cause an increase in animal mortality due to the impact of toxicity, thereby reducing the success rate of modeling, thus limiting its application

Method used

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  • Method for rapidly screening small experimental fish to construct pancreatic fat infiltration model
  • Method for rapidly screening small experimental fish to construct pancreatic fat infiltration model
  • Method for rapidly screening small experimental fish to construct pancreatic fat infiltration model

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Embodiment 1: Taking the common small experimental fish zebrafish and the mullet goby of the present invention as examples for comparison and screening

[0059] 25 120-200-day-old AB zebrafish and 18-20-generation Zhushi mullet gobies were selected respectively, and they were adaptively raised in a circulating water system for 5-8 days, and fed with biological bait and self-prepared feed every day.

[0060] The parts by weight of each raw material in the self-prepared feed (CK2 in the same embodiment 2) are: white fish meal 55g, soybean meal 15g, fish oil 0.75g, soybean oil 0.75g, potato starch 22g, carboxymethyl cellulose 2g, microcrystalline Cellulose 2.5g, and appropriate amount of mineral salt premix and vitamin premix.

[0061] Preferably, according to the final 1kg feed, it also includes a mineral salt premix, and its components are: 500 mg of sodium chloride; 7,500 mg of magnesium sulfate; 12,500 mg of sodium dihydrogen phosphate; 16,000 mg of potassium dihydroge...

Embodiment 2

[0086] Example 2: A method for constructing a pancreatic fat infiltration model by taking a closed group of male mullet gobies as an example

[0087] Select 250 180-200-day-old closed group male mullet gobies with a body weight of 0.35-0.50 g / tail. 12h light and night cycle, water temperature 24±2℃, pH 7.0±0.5, ammonia nitrogen ≤0.5mg L -1 , residual chlorine ≤ 0.05mg L -1 .

[0088] Prepare Zhushi mullet goby high-fat feed I, wherein the parts by weight of each raw material are: white fish meal 55g, soybean meal 15g, fish oil 6.5g, soybean oil 6.5g, potato starch 10.5g, carboxymethyl cellulose 2g, micro Crystalline cellulose 2.5g, and appropriate amount of mineral salt premix and vitamin premix.

[0089] Preferably, according to the final 1kg high-fat feed, it also includes a mineral salt premix, and its components are: 500 mg of sodium chloride; 7,500 mg of magnesium sulfate; 12,500 mg of sodium dihydrogen phosphate; 16,000 mg of potassium dihydrogen phosphate; Calcium d...

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Abstract

The invention discloses a method for rapidly screening a small experimental fish to construct a pancreatic fat infiltration model, and belongs to the technical field of biology. The pancreatic fat infiltration animal model is constructed by feeding mugilogobius chulae with high-fat feed. According to the method, the experimental fish of mugilogobius chulae which meets modeling expectation (havingstable / susceptible metabolic syndromes such as NAFLD) or is easy to model is screened out through an experiment, the liver pathological characteristics of the experimental fish are highly similar to those of a person in the early stage of NAFLD attacking and the early stage of liver fibrosis, and since NAFLD frequently occurs in clinical common pancreatic fat infiltration, the pathological state of the metabolic syndromes can be well simulated. The invention further discloses a reliable model evaluation method on the basis of ensuring high simulation, good repeatability, high reliability and low cost. The establishment of the evaluation method is beneficial for guiding technicians in the field in quickly constructing pancreatic fat infiltration animal models of mugilogobius chulae.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for quickly screening small experimental fish to construct a pancreatic fat infiltration model. Background technique [0002] "Pancreatic fatty infiltration" occurs when there is excess triglyceride deposition in the pancreas, or when there is fat replacement in the pancreatic tissue. At present, there is no unified nomenclature for fat deposition in the pancreas, and the common names are "fatty pancreas", "nonalcoholic fatty pancreatopathy", and "fatty infiltration of the pancreas", etc. (Lee et al., 2009; Mathur et al., 2007 ; Tushuizen et al., 2007). Studies have shown that pancreatic fat infiltration is mostly caused by obesity. Non-alcoholic fatty pancreas is mainly caused by acquired obesity and metabolic syndrome, including hyperglycemia, blood lipid disorders, hyperinsulinemia with insulin resistance, etc., which can be manifested in the early st...

Claims

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Application Information

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IPC IPC(8): A01K61/10A23K50/80A23K10/22A23K10/37A23K20/158A23K20/163A23K20/174A23K20/22A23K20/24A23K20/26A23K20/20
CPCA23K10/22A23K10/37A23K20/158A23K20/163A23K20/174A23K20/22A23K20/24A23K20/26A23K20/30A23K50/80A01K61/10Y02P60/87Y02A40/81Y02A40/818
Inventor 黄韧魏远征李建军蔡磊苗宗余余露军
Owner GUANGDONG LAB ANIMALS MONITORING INST
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