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Application of spina gleditsiae to preparation of antioxidant or nitric oxide release inhibitor

An anti-oxidant and nitric oxide technology, applied in anti-toxic, anti-inflammatory, anti-infective, etc.

Inactive Publication Date: 2019-02-15
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no research report on the application of saponins in anti-oxidation, and the application of saponins in anti-oxidation is of great significance for expanding the efficacy of saponins

Method used

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  • Application of spina gleditsiae to preparation of antioxidant or nitric oxide release inhibitor
  • Application of spina gleditsiae to preparation of antioxidant or nitric oxide release inhibitor
  • Application of spina gleditsiae to preparation of antioxidant or nitric oxide release inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The present embodiment measures DPPH free radical scavenging activity by the following method

[0035] (1) Preparation of DPPH solution and test solution

[0036] DPPH · Make 2×10 with absolute ethanol -4 The solution of M was stored at 4°C in the dark. Each monomeric compound and positive drug was made into 1 mg / mL stock solution with absolute ethanol, and diluted with absolute ethanol to the required concentration before testing.

[0037] (2) Determination of activity

[0038] Referring to the experimental method in the literature and improving it, 100 μL of different concentrations of the test solution and 2 × 10 -4 Add 100 μL of MDPPH solution to each well of a 96-well plate, and simultaneously use the concentrations of the test solution without adding DPPH (100 μL absolute ethanol instead of DPPH) as a control to eliminate the interference of the color of the test product itself on the test results. A DPPH·negative control was also set up (100 μL absolute ethanol...

Embodiment 2

[0048] The present embodiment measures the oxygen free radical scavenging capacity by the following method

[0049] (1) preparation of reaction reagent and need testing solution

[0050] Fluorescein disodium with 75mM potassium phosphate buffer (75mM KH 2 PO 4 , 75mM K 2 HPO 4 ) to prepare a 63 μM stock solution, store at 4°C in the dark, and dilute 100 times with this buffer solution before testing. AAPH was made into 18.3mM solution with 75mM potassium phosphate buffer before the experiment. Trolox was formulated with 75mM potassium phosphate buffer solution to make a 10μM stock solution, which was diluted to the required concentration before testing. Each monomeric compound and the positive drug VC were made into a 100 mM stock solution with 75 mM potassium phosphate buffer solution, and diluted to the required concentration with the buffer solution before testing.

[0051] (2) Determination of activity

[0052] The ORAC determination used in this experiment refers t...

Embodiment 3

[0059] In this embodiment, the activity of inhibiting rat macrophage NO release was measured by the following method

[0060] Since NO is extremely unstable, it is quickly oxidized to Stable in nature, it can be determined by Indirectly reflect the production of NO. In this paper, the Griess method was used to determine the content, the principle is that under acidic conditions, Diazotization with p-aminobenzenesulfonamide (Sulfanilamide), and then coupling reaction with N-1-naphthylene-diamine dihydrochloride (N-1-naphthylene-diamine dihydrochloride) to generate a purple azo dye, which is in There is a maximum absorption spectrum at 570nm, and its absorbance can be measured with an enzyme-linked immunosorbent assay. Determination by Griess method With the characteristics of high sensitivity, high accuracy and reproducibility, and the method is simple and effective, it is currently the most commonly used assay content, and methods of measuring NO levels.

[0061] ...

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Abstract

The invention provides application of spina gleditsiae to preparation of an antioxidant or a nitric oxide release inhibitor. By virtue of two in-vitro antioxidant activity evaluation methods such as aDPPH (1,1-Diphenyl-2-Picrylhydrazyl) radical scavenging method and an oxygen radical absorbance capacity (ORAC) method and an activity test for inhibiting NO release of rat macrophages, results showthat compared with rutin, hawthorn extract and other substances, the spina gleditsiae has equivalent or even higher activity, can serve as a novel antioxidant or nitric oxide release inhibitor to be used, and provides a novel conception and strategy for developing novel antioxidant drugs.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry and relates to the application of saponins in the preparation of antioxidants or nitric oxide release inhibitors. Background technique [0002] Modern research shows that many processes of life activities involve the generation and removal of free radicals. Free radicals are necessary for maintaining life in normal life, but free radicals are also dangerous killers of biological macromolecules (such as proteins, enzymes and DNA, etc.), cells and biological tissues. Under normal physiological conditions, the production and removal of free radicals in organisms are in a balanced state. Once the balance is out of balance, excess free radicals will attack target organs, causing cell membrane lipid peroxidation, nucleic acid backbone, protein and polypeptide bond breaks, cell apoptosis, etc., causing body aging and organ degenerative changes, leading to cardiovascular and cerebrovascular diseases ,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/483A61P39/06A61P31/00A61P29/00A61P37/02A61P9/02A61P11/06
CPCA61K36/483A61P9/02A61P11/06A61P29/00A61P31/00A61P37/02A61P39/06
Inventor 江坤鲁艺王铁杰殷果王珏李菁金一宝王丽君刘凯双王思明
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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