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Candida utilis and preparation method thereof

A technology for producing Candida utilis and mother liquor, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of waste of energy, manpower, material and financial resources, waste, and low added value of products, etc. The effect of increasing the added value of products

Inactive Publication Date: 2019-02-22
青海珠峰冬虫夏草工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The nutrient solution obtained by fermenting Cordyceps sinensis and separating the mycelia through the fermentation process is rich in a large amount of nutrients, mainly composed of protein, amino acid, polysaccharide, etc. At present, there is no comprehensive development and utilization, and it is only treated as fertilizer. The waste of resources is serious and the added value of the product is very low. , if it is discarded directly, it will be more wasteful, and it will cause environmental pollution. It needs special treatment such as sterilization before it can be discharged, and the cost is high.
This not only wastes a lot of energy, manpower, material and financial resources, but also poses a safety hazard to the environment.

Method used

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  • Candida utilis and preparation method thereof

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preparation example Construction

[0031] Further, the Candida utilis is prepared by the following steps:

[0032] (1) Take Cordyceps sinensis fermentation nutrient solution as the mother solution, add 20-40g / L carbon source, 3-7g / L nitrogen source and 1-3g / L inorganic salt, adjust pH to 4.5-7, and obtain culture solution

[0033] (2) Sterilization: sterilize the culture medium;

[0034] (3) Fermentation: inoculate the cultured strain of Candida utilis into the above-mentioned culture solution with an inoculum amount of 7% to 10%, and ferment and cultivate it at 25 to 35°C for 14 to 22 hours to obtain final product.

[0035] Preferably, the Candida utilis is obtained by the following steps:

[0036] (1) take Cordyceps sinensis fermentation nutrient solution as mother liquor, add 40g / L carbon source, 5g / L nitrogen source and 1g / L inorganic salt, adjust pH to 5.5, obtain culture medium;

[0037] (2) Sterilization: the culture medium is sterilized;

[0038] (3) Inoculation: Inoculate Candida utilis into the abo...

Embodiment 1

[0049]The used Candida utilis culture strain for inoculation is prepared by the following steps:

[0050] 1) Strain activation: Dilute the freeze-dried strain of Candida utilis with sterile water to make the original bacterial liquid, apply the original bacterial liquid on the wort solid medium, and incubate at 30°C for 5 1 day, pick a single colony and inoculate it into a slant test tube of wort solid medium, and transfer it once after the bacteria have grown to the entire slant of the test tube, and set aside;

[0051] 2) Pick strains from the slant culture medium, inoculate them in YPD medium (glucose 2%, peptone 2%, yeast extract powder 1%, the rest is distilled water), shake well, and place in a shaking incubator Fermentation culture was carried out on a shaker at 30° C. for 24 hours at a rotational speed of 130 r / min to form a culture strain of Candida utilis.

Embodiment 2

[0053] The used Candida utilis culture strain for inoculation is prepared by the following steps:

[0054] 1) Strain activation: Dilute the freeze-dried strain of Candida utilis with sterile water to make the original bacterial liquid, apply the original bacterial liquid on the wort solid medium, and cultivate it at 35°C for 3 1 day, pick a single colony and inoculate it into a slant test tube of wort solid medium, and transfer it once after the bacteria have grown to the entire slant of the test tube, and set aside;

[0055] 2) Pick strains from the slant culture medium, inoculate them in YPD medium (glucose 2%, peptone 2%, yeast extract powder 1%, the rest is distilled water), shake well, and place in a shaking incubator Fermentation culture was carried out in a shaker at 35° C. for 20 h at a rotational speed of 150 r / min to form a culture strain of Candida utilis.

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Abstract

The invention relates to the technical field of fungal fermentation, in particular to a candida utilis and a preparation method thereof. The candida utilis is cultured and prepared by a culture mediumcontaining aweto fermentation nutrient solution. The aweto fermentation nutrient solution serves as mother solution, carbon sources, nitrogen sources and inorganic salt are added to obtain culture solution, the culture solution is sterilized, candida utilis culture strains are inoculated into the culture solution, and fermentation culture is performed to obtain the candida utilis. As the aweto fermentation nutrient solution contains a large number of carbon sources and nitrogen sources and some metabolic organic components, the aweto fermentation nutrient solution is the good culture medium of the candida utilis, parts of nutrient substances needed by growth of the candida utilis can be supplemented, the aweto fermentation nutrient solution serves as the mother solution for culturing thecandida utilis, raw materials of the culture medium can be greatly saved, the aweto fermentation nutrient solution is maximally used, the added value of products is improved, and resources are reasonably used.

Description

technical field [0001] The invention relates to the technical field of fungal fermentation, in particular to Candida utilis and a preparation method thereof. Background technique [0002] Candida utilis, also known as torula utilis or edible torula, is a fungus with simple structure and mainly budding. It has large cells, vigorous metabolism, fast growth and reproduction, and can It forms better flocs, is easy to apply to a variety of different bioreactors, and has strong adaptability to the environment. The cells of Candida utilis are round, oval or sausage-shaped, and reproduce asexually by multilateral budding, forming pseudohyphae. [0003] Candida utilis is one of the most widely used microorganisms in humans. Candida utilis is widely used in food, In medicine, experimental research and animal husbandry. Along with the development of animal husbandry, the protein feed that guarantees the necessity of livestock is more and more scarce. Serious lack of feed protein h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K20/147C12R1/72
CPCA23K20/147C12N1/16
Inventor 李俊林王玉华董笑菲陈存霞
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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