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Application of rubus corchorifolius to preparation of antioxidants

An anti-oxidant and anti-oxidation technology, applied in anti-toxic agents, medical preparations containing active ingredients, plant raw materials, etc., to achieve excellent activity effects

Inactive Publication Date: 2019-03-08
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are few reports on the research on the medicinal aspects of Rubus, especially the research and development of the application of Rubus in anti-oxidation

Method used

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  • Application of rubus corchorifolius to preparation of antioxidants
  • Application of rubus corchorifolius to preparation of antioxidants
  • Application of rubus corchorifolius to preparation of antioxidants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The present embodiment measures DPPH free radical scavenging activity by the following method

[0035] (1) Preparation of DPPH solution and test solution

[0036] DPPH · Make 2×10 with absolute ethanol -4 The solution of M was stored at 4°C in the dark. Each monomeric compound and positive drug was made into 1 mg / mL stock solution with absolute ethanol, and diluted with absolute ethanol to the required concentration before testing.

[0037] (2) Determination of activity

[0038] Referring to the experimental method in the literature and improving it, 100 μL of different concentrations of the test solution and 2 × 10 -4 Add 100 μL of MDPPH solution to each well of a 96-well plate, and simultaneously use the concentrations of the test solution without adding DPPH (100 μL absolute ethanol instead of DPPH) as a control to eliminate the interference of the color of the test product itself on the test results. A DPPH·negative control was also set up (100 μL absolute ethan...

Embodiment 2

[0048] The present embodiment measures the oxygen free radical scavenging capacity by the following method

[0049] (1) preparation of reaction reagent and need testing solution

[0050] Fluorescein disodium with 75mM potassium phosphate buffer (75mM KH 2 PO 4 , 75mM K 2 HPO 4 ) to prepare a 63 μM stock solution, store at 4°C in the dark, and dilute 100 times with this buffer solution before testing. AAPH was made into 18.3mM solution with 75mM potassium phosphate buffer before the experiment. Trolox was formulated with 75mM potassium phosphate buffer solution to make a 10μM stock solution, which was diluted to the required concentration before testing. Each monomeric compound and the positive drug VC were made into a 100 mM stock solution with 75 mM potassium phosphate buffer solution, and diluted to the required concentration with the buffer solution before testing.

[0051] (2) Determination of activity

[0052] The ORAC determination used in this experiment refers t...

Embodiment 3

[0058] The present embodiment measures the content of each component of the Rubus with sample number 1-4 in Table 1 by liquid chromatography

[0059] Agilent ZORBAX SB-Aq C18 (250mm×4.6mm, 5μm) chromatographic column was used, the mobile phase was gradient elution of methanol-0.1% acetic acid solution, the detection wavelength was 254nm, the column temperature was 35°C, and the flow rate was 1.0mL / min. When the volume is 10 μL, the resolution and peak shape of the chromatogram obtained are better, and the number of chromatographic peaks is more and the distribution is uniform. Record the chromatogram to 2 times the retention time (180min), and no chromatographic peak appears after 90min, so the analysis time is 90min, and the peak area is recorded. The results are shown in Table 4 (wherein the sample number corresponds to the sample represented by the sample number in Table 1):

[0060] Table 4

[0061]

[0062] It can be seen from Table 4 that Rubus contains many complex...

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Abstract

The invention provides application of rubus corchorifolius to preparation of antioxidants. The rubus corchorifolius has good anti-oxidization performance, and can be used as a good antioxidant to achieve the goal of regulating and controlling the active oxygen metabolism in the body; through the detection on the anti-oxidization activity of the rubus corchorifolius, compared with rutin, hawthornsand the like, the rubus corchorifolius shows excellent activity; the DPPH free radical clearing rate can reach 50 to 70 percent; the ORAC value can reach 1.10 to 1.40; a novel idea is provided for thedevelopment of novel antioxidants and relevant anti-oxidization medicine.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry and relates to the application of Rubus in the preparation of antioxidants. Background technique [0002] Modern research shows that many processes of life activities involve the generation and removal of free radicals. Free radicals are necessary for maintaining life in normal life, but free radicals are also dangerous killers of biological macromolecules (such as proteins, enzymes and DNA, etc.), cells and biological tissues. Under normal physiological conditions, the production and removal of free radicals in organisms are in a balanced state. Once the balance is out of balance, excess free radicals will attack target organs, causing cell membrane lipid peroxidation, nucleic acid backbone, protein and polypeptide bond breaks, cell apoptosis, etc., causing body aging and organ degenerative changes, leading to cardiovascular and cerebrovascular diseases , cancer, diabetes, Parkinson's disease...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61P39/06
CPCA61K36/73A61P39/06
Inventor 王珏金一宝王铁杰江坤殷果李菁王丽君刘凯双王思明
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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