Preparation method of microlipid granule for wrapping medicine
A manufacturing method and technology of liposomes, applied in drug combination, drug delivery, pharmaceutical formulation, etc., can solve the problems of reducing curative effect, precipitation, leakage, etc., and achieve the effects of improving curative effect, avoiding agglutination, increasing stability and storage period
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Embodiment 1
[0012] Example 1: Preparation of liposomes coated with ammonium sulfate:
[0013] Unilamellar vesicles were fabricated using standard film hydration and repeated extrusion methods. Dipamitoylphosphatidyl choline (DPPD)], distearyl phosphatidyl choline (DSPC) and cholesterol [Cholesterol (Chol] (for example: distearyl Acid phosphocholine: cholesterol is 3: 2 or dipalmitate phosphocholine: cholesterol is 3: 2, the mole ratio) is dissolved in chloroform. After mixing, remove the solvent with a desiccator under reduced pressure, and then add 150mM [ Ammonium sulfate (NH 4 ) SO 4 ], pH 5.0, hydrated at 55°C, and the uniform mixture was repeatedly frozen and thawed five times with liquid nitrogen and a water bath, then filtered five times with a 0.1 μm polycarbonate filter membrane with a squeezer at 60°C, and then Filter five times with a 0.05 μm filter membrane to make ammonium sulfate-coated liposomes.
Embodiment 2
[0014] Embodiment 2: Preparation of liposomes encapsulating doxorubicin:
[0015] Ammonium sulfate-coated liposomes were used to remove sulfuric acid outside the liposomes with a molecular sieve column (Sephadex G-50, Pharmacia), and 1 mg of ezyme was added to every 10 μmole of phosphate lipid, heated to 60°C and shaken (100 rpm) for 30 Minutes, then rapidly cooled with ice cubes for 3 minutes, after cooling down, remove the epsin outside the liposomes with a molecular sieve column, and use a pH7.2 buffer (100mM DEPES, 144mM NaCl, pH7.2) or 0.9% sodium chloride solution, and the liposome part was collected to complete the preparation of the liposome. The completed liposomes were filtered with a 0.22 μm filter membrane and argon gas was passed through to facilitate preservation.
Embodiment 3
[0016] Embodiment 3: Preparation of liposomes encapsulating topotecan (topotecan):
[0017] Use a molecular sieve column (Sephadex G-50, Pharmacia) to remove the ammonium sulfate outside the liposomes, add 1 mg of topogen per 10 μmole of phosphate, heat to 60°C and shake (100rpm) 30 minutes, then rapidly cooled with ice cubes for 3 minutes, after cooling, use a molecular sieve column to remove the topogens outside the vesicles, and use a pH7.2 buffer solution (100mM DEPES, 144mM Nacl, pH7.2) for the eluent Or 0.9% sodium chloride solution, collect the liposome part, that is, complete the preparation of the liposome, and filter the completed liposome with a 0.22 μm filter membrane, and pass through argon gas to facilitate preservation.
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