Method for purifying EPA (eicosapentaenoic acid) from fish oil

A technology of EPA-C18 and fish oil, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of inability to separate EPA and DHA, ineffective separation of EPA and DHA, slow hydrolysis speed, etc., and it is not easy to achieve Oxidation, easy operation, high recovery effect

Active Publication Date: 2019-03-08
SEPAX TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0031] This method can selectively hydrolyze triglycerides containing a variety of fatty acids. The rate of hydrolysis of triglycerides containing EPA and DHA by lipase is slower than that of triglycerides without both, thus enriching more unsaturated fatty acid triglycerides, but cannot separate EPA and DHA
[0032] EPA and DHA cannot be effectively separated by a single method, and multiple methods need to be used in combination

Method used

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  • Method for purifying EPA (eicosapentaenoic acid) from fish oil
  • Method for purifying EPA (eicosapentaenoic acid) from fish oil
  • Method for purifying EPA (eicosapentaenoic acid) from fish oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1 Fish oil crude product analysis before purification

[0101] The crude fish oil has a purity of 70%.

[0102]Chromatographic column: Sepax GP-C18 (5um, 4.6x 250mm)

[0103] mobile phase:

[0104] A: water

[0105] B: Acetonitrile

[0106] Flow rate: 1ml / min Injection volume: 5ul Column temperature: RT

[0107] Sample: 1mg / ml (dissolved in 82% methanol) Pressure: 78bar

[0108] Instrument: Agilent 1260

[0109] Detection wavelength: UV@214nm

[0110] Elution was performed according to the following gradient:

[0111] Table 2

[0112] Time min)

[0113] The crude product analysis diagram is as follows figure 2 shown.

Embodiment 2

[0114] Embodiment 2 fish oil crude product purification

[0115] The crude fish oil has a purity of 20%.

[0116] Chromatographic column: EPA-C18 (1000x 250mm)

[0117] mobile phase:

[0118] A: water

[0119] B: Methanol

[0120] Flow rate: 400ml / min Injection volume: 400ml Column temperature: RT

[0121] Sample: 100mg / ml Pressure: 1.2MPa

[0122] Instrument: Sepax preparative chromatograph

[0123] Detection wavelength: UV@214nm

[0124] Gradient: 82%B

[0125] The specific method is as follows:

[0126] (1) Sample weighing: weigh 40 g of fish oil crude product on an analytical balance;

[0127] (2) Solvent preparation: prepare 400ml of 82% methanol, measure 328ml of methanol, and measure 72ml of purified water, mix the two together, and stir evenly, prepare two parts, and set aside;

[0128] (3) Sample dissolution: add one part of the solvent prepared in (2) above to (1) 40g of crude fish oil, seal it with plastic wrap, add a stir bar, and keep stirring on a magne...

Embodiment 3

[0136] Get fish oil crude product 40g, wherein EPA content 70% (m / m), add 82% methyl alcohol (V / V) 400ml, stir and make fully dissolving; Upper chromatographic column EPA-C18 (100mm * 250mm , containing packing quality about 1.18kg), sample loading flow rate 200ml / min, elution with 82% methanol after sample loading, elution flow rate 400ml / min, elution total 100min, HPLC detection and collection of EPA component solution, membrane concentration, used Nanofiltration membrane area 2.54m 2 , molecular weight cut off 150~300, membrane flux 20L / (m 2 · h), the temperature is controlled at 0-50° C., and the concentrated component solution is dried to obtain 26.2 g of EPA product. The purity detected by HPLC is 97.3%, and the recovery rate is 91.1%.

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Abstract

The invention relates to the field of analysis, in particular to a method for purifying EPA (eicosapentaenoic acid) from fish oil. The method has high selectivity and separation efficiency, EPA, DHA and other impurities can be effectively separated, unsaturated fatty acid and impurities in the fish oil are selectively adsorbed, sample loads are high, a moving phase of an organic solvent or organicsolvent/water system is used, the solvent can be recycled, and industrialization cost is reduced.

Description

technical field [0001] The invention relates to the field of analysis, in particular to a method for purifying EPA from fish oil. Background technique [0002] Eicosapentaenoic acid is a very effective anti-vascular disease drug discovered in recent years, which mainly exists in marine organisms. Eicosapentaenoic acid (EicosaPentaenoic-acid), referred to as EPA, is an omega-3 polyene fatty acid, which can be converted into prostaglandin I: (PGI.) with anticoagulant function in humans and animals and can inhibit thrombus The generation of factor A: (TXA:) has the effect of anti-vascular disease, and its side effects are small, so it is a promising anti-vascular disease drug. EPA is called "vascular scavenger". It has the functions of improving blood circulation, softening blood vessels, regulating blood lipids, lowering blood pressure and blood sugar, and is suitable for middle-aged and elderly people. Clinical trials have affirmed the medicinal effect of EPA, and increasin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C51/47C07C57/03
CPCC07C51/42C07C51/47C07C57/03
Inventor 王艺彭凯黄学英
Owner SEPAX TECH
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