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Multifunctional antibacterial polypeptide modified nano-silver compound and preparation method thereof

An antibacterial polypeptide and multi-functional technology, which can be used in antibacterial drugs, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. The effect of antibacterial effect

Active Publication Date: 2019-03-15
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application as a single inorganic nanomaterial is limited, so the inventors designed a multifunctional and targeted antibacterial peptide to modify nanosilver, which acts on the cell membrane and gathers at the site of inflammation and infection to jointly antibacterial

Method used

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  • Multifunctional antibacterial polypeptide modified nano-silver compound and preparation method thereof
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  • Multifunctional antibacterial polypeptide modified nano-silver compound and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The preparation method of the multifunctional antibacterial polypeptide modified nano-silver complex (MFP@AgNPs) of the present invention is as follows:

[0026] A multifunctional antimicrobial peptide composed of N-acetyl-acid-glycine-proline (N-Ac-PGP), polyethylene glycol (PEG), enzyme-sensitive peptide (ESP) and Tacyplesihn-1β-hairpin cation Peptide composition, synthesized by peptide solid-phase synthesis.

[0027] An appropriate amount of the synthesized multifunctional antimicrobial peptide was dissolved in water to make a certain concentration for use, and nano-silver (purchased from Zhongke Leiming (Beijing) Technology Co., Ltd.) was made with water to make a certain mass concentration for use. According to the mass concentration ratio is C AgNPs :C MFP =1:0.5, each 500μL was made into a volume of 1mL, placed overnight at 25°C, 200rpm, to fully combine. Obtain the desired complex, aliquot it, and store it at -20°C for later use.

Embodiment 2

[0029] The preparation method of the multifunctional antibacterial polypeptide modified nano-silver complex (MFP@AgNPs) of the present invention is as follows:

[0030] A multifunctional antimicrobial peptide composed of N-acetyl-acid-glycine-proline (N-Ac-PGP), polyethylene glycol (PEG), enzyme-sensitive peptide (ESP) and Tacyplesihn-1β-hairpin cation Peptide composition, synthesized by peptide solid-phase synthesis.

[0031] An appropriate amount of the synthesized multifunctional antimicrobial peptide is dissolved in water to make a certain concentration for use, and the nano silver is made with water to make a certain mass concentration for use. According to the mass concentration ratio is C AgNPs :C MFP =1:1, each 500μL was made into a volume of 1mL, and placed overnight at 25°C and 200rpm to fully combine. Obtain the desired complex, aliquot it, and store it at -20°C for later use.

Embodiment 3

[0033] The preparation method of the multifunctional antibacterial polypeptide modified nano-silver complex (MFP@AgNPs) of the present invention is as follows:

[0034] A multifunctional antimicrobial peptide composed of N-acetyl-acid-glycine-proline (N-Ac-PGP), polyethylene glycol (PEG), enzyme-sensitive peptide (ESP) and Tacyplesihn-1β-hairpin cation Peptide composition, synthesized by peptide solid-phase synthesis.

[0035] An appropriate amount of the synthesized multifunctional antimicrobial peptide is dissolved in water to make a certain concentration for use, and the nano silver is made with water to make a certain mass concentration for use. According to the mass concentration ratio is C AgNPs :C MFP =1:1.5, each 500μL was made into a volume of 1mL, placed overnight at 25°C, 200rpm, to fully combine. Obtain the desired complex, aliquot it, and store it at -20°C for later use.

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Abstract

The invention relates to the field of antibacterial drug research, in particular to a multifunctional antibacterial polypeptide modified nano-silver compound and a preparation method of the multifunctional antibacterial polypeptide modified nano-silver compound, so that antibacterial peptide and nano-silver jointly produce a synergistic antibacterial effect. The compound is obtained by electrostatically combining multifunctional antimicrobial peptide for designing Tacyplesihn-1 with nanosilver. The antibacterial peptide is modified by utilizing own excellent antibacterial activity, thereby being targetable and increasing the hydrophilicity of the antibacterial peptide, enzyme sensitive peptide can be used as a cutting substrate of a matrix metalloproteinase 2 / 9(MMP-2, MMP-9) to provide a basis for developing delivery vectors with specific biodegradable environments. Silver nanoparticles have inhibitory and bactericidal effects, and the combination of the enzyme sensitive peptide and the silver nanoparticles produces the synergistic antibacterial effect to solve the problem of strong drug resistance of drug-resistant strains in the prior art to affect the treatment of diseases.

Description

technical field [0001] The invention relates to the field of antibacterial drug research, in particular to a compound of multifunctional antibacterial polypeptide modified nano-silver and a preparation method thereof, so that the antibacterial peptide and nano-silver jointly produce a synergistic antibacterial effect. Background technique [0002] Antibiotic-resistant bacteria are currently a growing threat to human health, such as multidrug-resistant Gram-negative bacteria Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii and Gram-positive bacteria resistant to methicillin Superbugs like Staphylococcus aureus kill hundreds of thousands of people every year and generate enormous medical costs. The rapid rise in bacterial resistance to antibiotics necessitates the need for new therapeutic strategies, and this can be accomplished through the development of novel antimicrobial compounds and efficient intracellular drug delivery systems. Most antibiotics target s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/38A61K47/64A61K38/06A61P31/04A61K47/55
CPCA61K33/38A61K38/06A61K47/55A61K47/64A61P31/04A61K2300/00
Inventor 赵永星李文茜张楠苏静静赵艺丹
Owner ZHENGZHOU UNIV
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