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Preparation method of dual-emission colorimetric fluorescence nano-microsphere and application of nano-microsphere in bacterial detection

A fluorescent nanometer and nanometer microsphere technology, applied in the field of colorimetric sensing, can solve problems such as influence, and achieve the effects of cheap raw materials, simple method, and easy industrial production.

Active Publication Date: 2019-03-19
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, most of these fluorescence detection devices are based on single-emission detection, which means that many factors including probe concentration, instrument sensitivity, and environmental conditions can affect the detection results.

Method used

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  • Preparation method of dual-emission colorimetric fluorescence nano-microsphere and application of nano-microsphere in bacterial detection
  • Preparation method of dual-emission colorimetric fluorescence nano-microsphere and application of nano-microsphere in bacterial detection
  • Preparation method of dual-emission colorimetric fluorescence nano-microsphere and application of nano-microsphere in bacterial detection

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) The specific synthesis method of blue fluorescent carbon dots (CDs) is as follows: 1.0507 g of citric acid and 335 μL of ethylenediamine were dissolved in 10 mL of deionized water. Then the mixed solution was added into a Teflon reactor, heated to 200° C. for 5 hours, and naturally cooled to room temperature after the reaction. The obtained product is a brown-black transparent solution, which is then treated by dialysis to remove unreacted small molecules, and finally obtains a yellow aqueous solution of carbon quantum dots. After freeze-drying, a purified light yellow CDs powder is obtained, which is dispersed in deionized water to prepare a concentration of 10mg / mL CDs solution, and store at 4°C in the dark for future use.

[0026] (2), blue fluorescent SiO 2 Preparation of @CDs: First, add 5mL of the CDs solution obtained in step (1) into a 1000mL reaction flask, add 1.5mL tetraethyl orthosilicate (TEOS), 13mL ammonia water and 150mL ethanol, mix well, blow in n...

Embodiment 2

[0032] (1) The specific synthesis method of blue fluorescent carbon dots (CDs) is as follows: 0.9006 g of lactic acid and 300 μL of urea were dissolved in 8 mL of deionized water. Then the mixed solution was added into a Teflon reactor, heated to 200°C for 6 hours, and cooled to room temperature naturally after the reaction. The obtained product is a brown-black transparent solution, and then treated by dialysis to remove unreacted small molecules, and finally obtain a yellow aqueous solution of carbon quantum dots, obtain purified light yellow CDs powder after freeze-drying, and disperse it in deionized water to prepare a concentration of 10 mg / mL CDs solution, and store at 4°C in the dark for future use.

[0033] (2), blue fluorescent SiO 2 Preparation of @CDs: First, add 10mL of the CDs solution obtained in step (1) into a 1000mL reaction flask, add 1mL tetraethyl orthosilicate (TEOS), 12mL triethylamine and 125mL methanol, mix well, and blow in nitrogen, The mixed soluti...

Embodiment 3

[0039] (1) The specific synthesis method of blue fluorescent carbon dots (CDs) is as follows: 1.0977 g of terephthalic acid and 350 μL of arginine were dissolved in 12 mL of deionized water. Then the mixed solution was added into a Teflon reactor, heated to 180° C. for 8 hours, and naturally cooled to room temperature after the reaction. The obtained product is a brown-black transparent solution, which is then treated by dialysis to remove unreacted small molecules, and finally obtains a yellow aqueous solution of carbon quantum dots. After freeze-drying, a purified light yellow CDs powder is obtained, which is dispersed in deionized water to prepare a concentration of 10mg / mL CDs solution, and store at 4°C in the dark for future use.

[0040] (2), blue fluorescent SiO 2 Preparation of @CDs: First, add 8mL of the CDs solution obtained in step (1) into a 1000mL reaction flask, add 1mL tetraethyl orthosilicate (TEOS), mix 10mL ammonia water and 120mL isopropanol, and blow in ni...

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Abstract

The invention relates to a preparation method of a dual-emission colorimetric fluorescence nano-microsphere and application of the nano-microsphere in bacterial detection, belonging to the technical field of colorimetric sensing. A SiO2 nano-microsphere loaded with blue fluorescence carbon dots (CDs) and red fluorescence Cu NCs prepared in situ by virtue of an assembling method and has a dual-emission fluorescence effect, namely that the nano-microsphere presents 450-nm blue fluorescence and 650-nm red fluorescence in a visible light region. The nano-microsphere has a specific response effectto the pH value, the red fluorescence of the nano-microsphere gradually weakens along the change of the pH value from 4.0 to 7.2, and meanwhile, the blue fluorescence remains unchanged, so that the visual colorimetric detection of the change of the pH value can be realized through the color change of compound light. A dual-emission colorimetric fluorescence nano-microsphere probe can be used for detecting the pH value of a bacterial metabolism system so as to acquire the breeding amount of bacteria, and the biofluorescence imaging of the bacteria can be realized in a relatively high signal tonoise ratio. The nano-microsphere can be separated from a detection system through a simple centrifugal operation, so that the environmental pollution caused by a probe material can be reduced.

Description

technical field [0001] The invention belongs to the technical field of colorimetric sensing, and in particular relates to the preparation of a pH-responsive dual-emission colorimetric fluorescent nanosphere probe and its application in the field of bacterial metabolism detection and imaging. Background technique [0002] Bacteria are ubiquitous in our daily lives. While some are beneficial to humans, such as Lactobacillus and Streptomyces, most bacteria do more harm than good, especially when it comes to food storage, life safety and physical health. 20% of food wasted around the world is caused by bacteria, which not only causes food waste and economic loss, but also causes environmental pollution. Simultaneously, the reproduction of various bacteria can cause various diseases and even endanger life. Escherichia coli (E. coli) is one of the most common clinical pathogens, causing a series of life-threatening diseases (such as sepsis and hemolytic uremic syndrome) and caus...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 林权宋善良王传洗赵月赵玥琪刘厚杨柏
Owner JILIN UNIV
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