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A kind of biological type periosteum repair material and preparation method thereof

A technology of mucosal layer and submucosa layer, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems without too much consideration of biological safety and practicability, so as to avoid cytotoxicity, good biocompatibility, The effect of promoting proliferation

Active Publication Date: 2021-07-16
SUMMIT GD BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies are still in the early research stage, and the issues of biosafety and practicality have not been considered too much
[0006] At present, in view of the important role of periosteum in bone repair, and there is no effective product for repairing periosteum to treat bone defects

Method used

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  • A kind of biological type periosteum repair material and preparation method thereof

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Embodiment 1

[0026] This embodiment provides a porcine small intestinal submucosa tissue acellular matrix material and a preparation method thereof. This preparation method comprises the steps:

[0027] Step 1. The jejunum section of the porcine small intestine was taken, cleaned and cut radially, and washed repeatedly with PBS solution to remove pollutants.

[0028] Step 2. Physically remove the mucous membrane, using a wooden scraper, carefully and evenly scrape off the mucous membrane layer of the inner layer of the SIS membrane.

[0029] Step 3. Physically remove the sarcolemma and serosa. The wooden scraper and scalpel are used together to remove the sarcolemma and serosa on the outer layer of the SIS membrane.

[0030] Step 4, configure 0.18% (mass fraction) peracetic acid solution, 0.18% (mass fraction) sodium hydroxide solution, 80% acetone solution;

[0031] Then first place the SIS membrane that removes the mucous membrane layer, sarcolemma layer and serosa layer into peracetic...

Embodiment 2

[0037] This embodiment provides a porcine small intestinal submucosa tissue acellular matrix material and a preparation method thereof. This preparation method comprises the steps:

[0038] Step 1. The jejunum section of the porcine small intestine was taken, cleaned and cut radially, and washed repeatedly with PBS solution to remove pollutants.

[0039] Step 2. Physically remove the mucous membrane, using a wooden scraper, carefully and evenly scrape off the mucous membrane layer of the inner layer of the SIS membrane.

[0040] Step 3. Physically remove the sarcolemma and serosa. The wooden scraper and scalpel are used together to remove the sarcolemma and serosa on the outer layer of the SIS membrane.

[0041] Step 4, configure 0.25% (mass fraction) peracetic acid solution, 0.25% (mass fraction) sodium hydroxide solution, 85% acetone (mass fraction) solution;

[0042] Then first place the SIS membrane that removes the mucous membrane layer, sarcolemma layer and serosa laye...

Embodiment 3

[0048] This embodiment provides a porcine small intestinal submucosa tissue acellular matrix material and a preparation method thereof. This preparation method comprises the steps:

[0049] Step 1. The jejunum section of the porcine small intestine was taken, cleaned and cut radially, and washed repeatedly with PBS solution to remove pollutants.

[0050] Step 2. Physically remove the mucous membrane, using a wooden scraper, carefully and evenly scrape off the mucous membrane layer of the inner layer of the SIS membrane.

[0051] Step 3. Physically remove the sarcolemma and serosa. The wooden scraper and scalpel are used together to remove the sarcolemma and serosa on the outer layer of the SIS membrane.

[0052] Step 4, configure the peracetic acid solution of 0.15% (mass fraction), the sodium hydroxide solution of 0.15% (mass fraction), the acetone solution of 75% (mass fraction);

[0053] Then first place the SIS membrane that removes the mucous membrane layer, sarcolemma la...

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Abstract

The invention relates to a biological type periosteum repair material and a preparation method thereof, in particular to an acellular matrix material, a preparation method thereof and an application in periosteum repair. The preparation method comprises: (1) taking pig small intestine jejunum section, cleaning, radially incision, removing mucosal layer, sarcolemma layer and serosa layer to obtain pig small intestine submucosa tissue membrane; The porcine small intestinal submucosa tissue membrane is processed as follows in sequence: first soaked in peracetic acid solution and washed; then soaked in sodium hydroxide solution and washed; finally soaked in acetone solution and washed to obtain porcine small intestinal mucosa The underlying tissue decellularized matrix. The invention has high biological safety, low immunogenicity and cytotoxicity; good biocompatibility, can promote the proliferation of cells adhered to it, and is suitable for use as a biological repair material, especially for periosteum repair.

Description

technical field [0001] The invention relates to the technical field of tissue repair materials, in particular to a biological periosteum repair material and a preparation method thereof, in particular to an acellular matrix material, a preparation method thereof and an application in periosteum repair. Background technique [0002] The periosteum is composed of microvascularized dense connective tissue that tightly covers the outer surface of the cortical bone through Sharpey's fibers, forming a thin but tough membrane. The periosteum is a highly vascularized tissue containing osteogenic and chondrogenic progenitor cells and other relevant bioactive factors, and is described as a well-vascularized "bone / cartilaginous organ" that provides blood circulation to bone and is considered is the "umbilical cord" of the bone, and periosteum peeling usually disrupts the arterial nutrient supply to the bone. Histologically, the periosteum is divided into two distinct layers: an outer ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/365A61L27/3687A61L2430/02A61L2430/40
Inventor 凌友杨景徐斌林祥贵
Owner SUMMIT GD BIOTECH
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