Catalyst for preparing polypeptide through ring-opening polymerization of amino acid N-carboxy anhydride, and method for preparing polypeptide by catalyst

Abstract

Disclosed are a catalyst for preparing polypeptide through ring-opening polymerization of amino acid N-carboxy anhydride, and a method for preparing the polypeptide by the catalyst. The catalyst comprises a component A and a component B, both of which are non-metal compounds; the component A is used for initiating ring-opening polymerization of an amino acid N-carboxy anhydride monomer, the molecular weight of the obtained polypeptide is controlled, the component B is used for activating the amino acid N-carboxy anhydride monomer, the polymerization reaction rate is controlled, and the component B can greatly reduce the reaction activation energy and accelerate the polymerization reaction. The method includes the steps of adding the component A and the component B of the catalyst and an organic solvent into an anhydrously treated polymerization bottle at the room temperature, adding the amino acid N-carboxy anhydride monomer after stirring, carrying out a polymerization reaction at theroom temperature, adding a hydrochloric acid alcohol solution to stop the reaction, pouring reaction liquid into ethyl alcohol for precipitation, performing filtration to obtain white solids, and drying the white solids to obtain the polypeptide. The method has the advantages of environment protection, high efficiency and accuracy.

Description

technical field [0001] The invention relates to the technical field of polypeptide catalysis, in particular to a catalyst for preparing polypeptide by ring-opening polymerization of acid anhydride in an amino acid ring and a method for preparing polypeptide by using the catalyst. Background technique [0002] Polypeptide is a kind of amino acid polymer, and the polypeptide can have certain specific functions through molecular structure design. Since the polypeptide can be degraded into small molecular amino acids for metabolism and absorption under the action of corresponding enzymes in the body, it has high biocompatibility and biomedical prospects. Peptides can effectively self-assemble into a stable and orderly high-level structure, which is the characteristic and advantage of peptides different from other biomedical materials, and this self-assembly ability just gives peptides unique life functions. The self-assembly ability of the polypeptide comes from its precise mai...

AI Technical Summary

Problems solved by technology

But the serious deficiency of this initiation system is: the initiation efficiency of the metal complex is low, and an expensive metal complex in the system can initiate the polymerization of one polymer chain at most, so the catalyst concentration is high, and the metal residue in the obtained polymer is high.

However, due to the low nucleophilicity of primary amine hydrochloride, the polymerization activity is extremely low (TOF value is only at 2.7h -1 About), even if the polymerization reaction is carried out at 40-80°C, it generally takes about three days to complete, so the controllability of molecular weight is not very good

The TOF value of the trimethylsilylamino compound-initiated system is comparable to that of the transition metal-initiated system, at about 13.3h -1 around, but the molecular weight distribution of the resulting polymer is broad (1.19-1.26)

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