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Method for separating and culturing seaweed protoplasts and regenerating plants

A technology for protoplasts and regenerated plants, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of short seedling cultivation period, achieve the effect of increasing division frequency, increasing cell division speed and maintaining stability

Active Publication Date: 2021-11-23
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for separating and cultivating seaweed protoplasts and regenerating plants, providing a method for cultivating seaweed with a short seedling period and high seed breeding efficiency, which can solve the problems of asexual seaweed seed preservation and breeding , can be used for productive seedling

Method used

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  • Method for separating and culturing seaweed protoplasts and regenerating plants

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Experimental program
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Effect test

Embodiment 1

[0023] Seaweed species choose Eucheuma.

[0024] The method for separating and cultivating seaweed protoplasts and regenerating plants is mainly based on the totipotency of seaweed somatic cell development, using seaweed tool enzymes to decompose seaweed tissues, freeing somatic cell protoplasts, obtaining somatic cells and cell lines that regenerate cell walls, and then Regenerated plants were obtained by culturing. Specific steps are as follows:

[0025] (1) Select algal bodies with normal color, no rot, and vigorous growth, use a brush to clean up the sludge, algae and other subsidiary impurities on the surface of the algae, and use 0.005% HgCl 2 The solution was sterilized for 5 seconds, then rinsed with sterilized seawater, cut off the young part of the top of the fresh algae, blotted the surface moisture with filter paper, cut or ground it into small tissue pieces or small branches, and firstly mixed potassium sodium tartrate and After soaking in the PVP mixed solution...

Embodiment 2

[0034] Seaweed algae species choose red leek.

[0035] The method for separating and cultivating seaweed protoplasts and regenerating plants is mainly based on the totipotency of seaweed somatic cell development, using seaweed tool enzymes to decompose seaweed tissues, freeing somatic cell protoplasts, obtaining somatic cells and cell lines that regenerate cell walls, and then Regenerated plants were obtained by cultivation. Specific steps are as follows:

[0036] (1) Select algal bodies with normal color, no rot, and vigorous growth, use a brush to clean the surface sludge, algae and other subsidiary impurities, and use 0.006% HgCl 2 The solution was sterilized for 6 seconds, then rinsed with sterilized seawater, cut off the tender part of the top of the fresh algae, blotted the surface moisture with filter paper, cut or ground it into small tissue pieces or small branches, and firstly mixed potassium sodium tartrate and After soaking in the PVP mixed solution for 2 hours, ...

Embodiment 3

[0045] Seaweed species choose seaweed.

[0046] The method for separating and cultivating seaweed protoplasts and regenerating plants is mainly based on the totipotency of seaweed somatic cell development, using seaweed tool enzymes to decompose seaweed tissues, freeing somatic cell protoplasts, obtaining somatic cells and cell lines that regenerate cell walls, and then Regenerated plants were obtained by cultivation. Specific steps are as follows:

[0047] (1) Select algae with normal color, no rot, and vigorous growth, use a brush to clean up the sludge, algae and other subsidiary impurities on the surface of the algae, and use 0.008% HgCl 2 The solution was sterilized for 10 seconds, then rinsed with sterilized seawater, cut off the young part of the top of the fresh algae, blotted the surface moisture with filter paper, cut or ground it into small tissue pieces or small branches, and firstly mixed potassium sodium tartrate and After soaking in the PVP mixed solution for ...

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Abstract

The invention provides a method for separating and cultivating seaweed protoplasts and regenerating plants, which belongs to the field of algae cultivation. The specific method is as follows: select algal bodies with normal color, no decay, and vigorous growth, and use a brush to clean the surface sludge, miscellaneous algae and other algae bodies. Auxiliary impurities, with HgCl 2 Solution disinfection, followed by rinsing with sterilized seawater, cutting off the tender part of the top of the fresh algae body, blot the surface moisture with filter paper, cutting or grinding it into fine tissue pieces or small branches, firstly in potassium sodium tartrate and poly Soak in vinylpyrrolidone mixed solution, then use seaweed tool enzyme solution for digestion and enzymolysis, and then centrifuge to obtain protoplasts of seaweed somatic cells; cultivate the protoplasts to obtain cells with regenerated cell walls, and further cultivate to obtain regenerated plants. The invention provides a method for cultivating seaweed with short seedling cultivation period and high seed breeding efficiency, which can solve the problems of seed preservation and propagation of asexual seaweed seed, and can be used for productive seedling cultivation.

Description

technical field [0001] The invention belongs to the field of algae cultivation methods, and in particular relates to methods for separating and cultivating seaweed protoplasts and regenerating plants. Background technique [0002] Although algae do not have flowers, fruits, seeds and other structures to reproduce offspring, they have various reproductive methods to adapt to the environment. In terms of asexual reproduction, some cells can be directly divided into two, such as Spirogyra, which can be broken into several segments, and each segment grows into an independent individual; some algae can produce many spores with flagellates, which can swim freely. After the spores mature, they grow into a new individual; when the environment is unfavorable, some algae can produce thick-walled dormant spores, and when the environment is suitable, they will germinate and grow into new individuals. In terms of sexual reproduction, some algae can produce male and female gametes, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王斌张建设杨静静
Owner ZHEJIANG OCEAN UNIV
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