Method applicable to isolation and introduction of infiltrating T lymphocytes in malignant solid tumors

Abstract

The invention provides a method applicable to isolation and introduction of infiltrating T lymphocytes in malignant solid tumors, in particular to comprise procedures as follows: 1) processing a tumortissue into a tissue mass with a volume of 0.5-3 mm<3>; 2) spreading a matrix glue in the cell culture plate and transfer the processed tumor tissue mass in step 1 to the matrix glue for coating; 3)transferring the coated tissue mass in step 2 to another cell culture plate and incubating for 10-60 minutes; 4) adding a pre-amplification medium to the cell culture plate in step 3 and carrying on the pre-amplification culture for 10-30 days and using the semi-liquid of the pre-amplification medium as an exchange per 2-3 days. The invention provides an effective method for isolation and introduction of infiltrating T lymphocytes in tumors for the treatment of specific biotheraphy of malignant solid tumors. The tumor infiltrating T lymphocytes isolated by the method have high purity and stronger killing activity of tumor cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for separating and inducing tumor infiltrating T lymphocytes suitable for malignant solid tumors. Background technique [0002] At present, the main treatment methods for tumor treatment include traditional radiation therapy, surgical resection of tumor, drug therapy and emerging tumor biological therapy. Since surgery, radiotherapy and chemotherapy will bring huge toxic and side effects to patients, biological therapy has become the fourth important tumor treatment mode due to its significant curative effect. Tumor biological therapy mainly includes: adoptive cell therapy, cytokine therapy, tumor vaccine, targeted molecular therapy, etc. At present, there are many studies on adoptive cell therapy, including tumor-infiltrating lymphocytes (tumor-infiltrating lymphocytes, TIL), which are mainly CD8 cells gathered in the tumor lesion area. + T lymphocytes are at the forefron...

AI Technical Summary

Problems solved by technology

These classic methods for isolating tumor-killing activity of TIL cells need to isolate and induce primary tumor cells as target cells from patient tumor tissues, and co-cultivate TIL cells and target cells in vitro (for example, 24 hours), which takes a long time. ; and isolated and cultured primary tumor cells from tumor tissue and local tumor infiltrating CD8 + T lymphocytes are also quite difficult. Therefore, it is urgent to establish an efficient and easy method to isolate and identify tumor infiltrating T lymphocytes in patients, so as to be applied to tumor immunotherapy

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