Isothermal amplification terahertz multi-channel microfluidic chip based on magnetorheological fluid technology and its method for pathogen detection

Abstract

The invention discloses a constant-temperature amplification terahertz multi-channel microfluidic chip based on magnetorheological fluid technology and a method for detecting pathogenic bacteria, including a rolling circle amplification (RCA) reaction module, magnetorheological Liquid exchange module, metamaterial nanoparticle signal amplification module and microfluidic chip structure. The microfluidic chip structure is a "compass-type" structure with multiple detection channels. There is a main entrance in the center. Each detection channel is connected from the inside to the outside with an RCA reaction module, a magnetorheological fluid exchange module and metamaterial nanoparticles. Signal amplification module. The detection method uses RCA, magnetorheological fluid and metamaterial nanoparticle signal amplification modules to solve the problem of water sensitivity and low sensitivity. As a new method for rapid and accurate detection of pathogenic bacteria, it is of great significance for the diagnosis and treatment of infectious diseases.

Description

technical field [0001] The invention relates to the field of pathogenic bacteria detection, in particular to a constant temperature amplification terahertz multi-channel microfluidic chip based on magnetorheological fluid technology, and also to a method for detecting pathogenic bacteria by using the microfluidic chip. Background technique [0002] Rapid and accurate detection of the species of pathogenic bacteria is crucial for the diagnosis and subsequent treatment of infectious diseases. Traditional pathogenic bacteria detection methods take a long time, from collecting specimens to accurately obtaining the type of pathogenic bacteria usually takes a few days to a week or so. In addition, the bacterial culture operation is cumbersome, and different bacteria require different enzymatic and biochemical reaction identifications, which consume a large amount of reagents. In order to quickly identify the species of pathogenic bacteria, a series of molecular biology methods in...

AI Technical Summary

Problems solved by technology

However, there are still problems of specificity, sensitivity and water sensitivity in the detection of pathogenic bacteria by using the existing THz wave detection technology.

(1) Specificity problem: The direct detection of pathogenic bacteria by THz waves is based on the principle that different species of pathogenic bacteria have different intracellular water content and intracellular structure. Although this method can distinguish pathogenic bacteria after purification and culture, it is essentially not Does not have high detection specificity

Considering that there are pathogenic bacteria with similar results or mixed with multiple pathogenic bacteria in clinical samples, the direct detection method cannot specifically distinguish the species of pathogenic bacteria in clinical samples

(2) Sensitivity problem: the previous research results show that without any signal amplification technology, THz spectrum can only detect 10 8 Pathogens above CFU

(3) Water sensitivity problem: at room temperature, the absorption coefficient of water to THz waves at 1THz frequency is as high as 250cm -1 left and right, the THz absorption signal of pathogenic bacteria in the liquid phase environment is often annihilated

But so far, there is no report on the application of magnetorheological fluid technology in the field of terahertz wave detection.

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