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Application of artesunate in preparation of drug for inhibiting tumor cell drug resistance

A tumor cell and artesunate technology, applied in the field of biomedicine, can solve problems such as unclear mechanism and damage, and achieve good cytotoxicity and induce cell apoptosis

Inactive Publication Date: 2019-04-12
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have shown that artesunate has a wide range of anti-tumor activities and has cytotoxic effects on a variety of cancer cells, but the mechanism is unclear and may be related to DNA damage

Method used

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  • Application of artesunate in preparation of drug for inhibiting tumor cell drug resistance
  • Application of artesunate in preparation of drug for inhibiting tumor cell drug resistance
  • Application of artesunate in preparation of drug for inhibiting tumor cell drug resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Effect of artesunate on tumor cell lysosome function

[0029] 1.1 Materials and methods

[0030] 1) Experimental materials

[0031]

[0032] 2) Experimental cells

[0033] Human breast cancer MCF-7 cells were purchased from the cell bank of the Institute of Cell Biology, Chinese Academy of Sciences and preserved in our laboratory; human cervical cancer Hela cells were purchased from the cell bank of the Institute of Cell Biology of the Chinese Academy of Sciences and preserved in our laboratory; human colon cancer HCT116 Cells were purchased from the Cell Bank of the Institute of Cell Biology, Chinese Academy of Sciences and preserved in our laboratory; human liver cancer HepG2 cells were purchased from the Cell Bank of the Institute of Cell Biology of the Chinese Academy of Sciences and preserved in our laboratory; human lung cancer A549 cells were purchased from the Cell Biology of the Chinese Academy of Sciences The cell bank of the research institute ...

Embodiment 2

[0046] Example 2 Relationship between lysosome function and drug resistance

[0047] 2.1 Effect of lysosomal synthesis-related transcription factor TFEB on the efficacy of cytotoxic drugs

[0048] TFEB is the main gene that regulates lysosomal biosynthesis, and can improve autophagy / lysosomal function by regulating the expression of autophagy / lysosomal biosynthesis-related genes. Therefore, we enhanced or weakened the function of lysosomes by up-regulating or down-regulating the level of TFEB, and further explored the effect of artesunate on cells.

[0049] 2.1.1 Materials and methods

[0050] 1) Experimental materials

[0051]

[0052] 2) Lentivirus

[0053] TFEB overexpression lentivirus and mutant lentivirus were constructed by Jikai Company: LV-TFEB (6778-1), (titer 1E+8TU / mL); negative control virus CON195 (Ubi-MCS-3FLAG-SV40-puromysin) , (titer is 1E+9TU / mL).

[0054] 3) siRNA

[0055] TFEB siRNAsense: 5'-GAGACGAAGGTTCAACATCAA-3'.

[0056] 2.1.2. Method and st...

Embodiment 3

[0070] Example 3 Comparison of lysosome functions in several drug-resistant cells

[0071] 3.1 Materials

[0072]

[0073] Human breast cancer cell MCF-7 / ADR resistant to doxorubicin was purchased from the cell bank of the Institute of Cell Biology, Chinese Academy of Sciences and preserved in our laboratory; human lung cancer cell A549 / TAX resistant to paclitaxel was donated by teacher Liang Zhongqin’s laboratory at Soochow University; human lung cancer The cisplatin-resistant cell A549 / DDP was donated by the laboratory of Mr. Liang Zhongqin of Soochow University.

[0074] 3.2 Methods and steps

[0075] 1) MCF-7 / ADR resistance maintenance

[0076] During the cell culture process, doxorubicin was added for maintenance, and the concentration of doxorubicin was increased by 0.01 μg / mL for each passage, depending on the state of the cells in the middle, and finally added to 2 μg / mL.

[0077] 2) A549 / DDP resistance maintenance

[0078] During the cell culture process, cispl...

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Abstract

The present invention discloses an application of artesunate in preparation of a drug for inhibiting tumor cell drug resistance, and at the same time discloses a pharmaceutical preparation prepared from the artesunate and a method for inhibiting the tumor cell drug resistance in vitro. Experiments show that lysosomal functions have a certain relationship with the tumor cell drug resistance and areone of mechanisms of the tumor cell drug resistance. The artesunate can inhibit the lysosomal functions, increases lysosomal pH, decreases lysosomal enzyme activity, reduces elimination of disablingmitochondria, and thus induces cell apoptosis. Therefore, the artesunate has a better cytotoxic effect on the drug resistance cells with the higher lysosomal functions, can be used as the drug for inhibiting the tumor cell drug resistance and is used for adjuvant treatment of tumors.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of artesunate in the preparation of drugs for inhibiting drug resistance of tumor cells. Background technique [0002] Malignant tumors are major diseases that seriously threaten human health. The research on the treatment of tumors is urgent, and its prevention and treatment has become the top priority in the medical field of our country. The development of malignant tumors includes multiple steps, and the main features are local invasion and distant metastasis, which are the main causes of clinical death in cancer patients. Chemotherapy is one of the effective means to treat malignant tumors, and it has a good therapeutic effect on some tumors. The overall survival of some patients with advanced tumors has been effectively prolonged after combined chemotherapy. But for most solid tumors, the objective effective rate of chemotherapy is still low....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/357A61P35/00C12N5/09
CPCA61K31/357A61P35/00C12N5/0693C12N2501/06
Inventor 林芳向敏李哲
Owner SUZHOU UNIV
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