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Method for extracting collagen from fish scales

A technology of collagen and fish scales, which is applied in the chemical industry, can solve the problems of drug residues and bacteria removal, drug residues and parvovirus removal, collagen is not pure enough, etc., to improve the extraction rate, maintain protein activity, and stabilize the properties of collagen Effect

Inactive Publication Date: 2019-04-12
广州华大生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this preparation method still does not effectively remove drug residues and bacteria in fish scales.
[0005] At present, most methods of obtaining collagen in fish scales are obtained by enzymatic hydrolysis after acid-base treatment, but in the process of obtaining collagen, some unavoidable drug residues and parvoviruses in fish scales have not been removed, and the obtained collagen is not enough. pure

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  • Method for extracting collagen from fish scales
  • Method for extracting collagen from fish scales

Examples

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Effect test

Embodiment 1

[0033] Embodiment 1 A kind of method that extracts collagen from fish scale

[0034] The described method for extracting collagen from fish scales comprises the following steps:

[0035] S1 Add water 7 times the weight of fish scales to fish scales, heat to 40°C, soak for 2 hours to obtain rehydrated fish scales;

[0036] S2 Add the rehydrated fish scales obtained in step S1 into 6% sodium hydroxide aqueous solution and soak for 30 hours, the quality of the sodium hydroxide aqueous solution is 3 times of the quality of the rehydrated fish scales, remove the sodium hydroxide aqueous solution to obtain degreasing fish scales;

[0037] S3 Soak the degreased fish scales obtained in step S2 in 10% hydrochloric acid aqueous solution for 2 hours, the quality of the hydrochloric acid aqueous solution is 4 times the mass of the degreased fish scales, and wash with distilled water until neutral to obtain pretreated fish scales;

[0038] S4 with step S3 gained pretreatment fish scale u...

Embodiment 2

[0045] Embodiment 2 A kind of method that extracts collagen from fish scale

[0046] The described method for extracting collagen from fish scales comprises the following steps:

[0047] S1 Add water 9 times the weight of fish scales to fish scales, heat to 50°C, soak for 2 hours to obtain rehydrated fish scales;

[0048] S2 Adding the rehydrated fish scale obtained in step S1 into 15% sodium hydroxide aqueous solution for soaking for 20 hours, the quality of the sodium hydroxide aqueous solution is 5 times the mass of the rehydrated fish scale, removing the sodium hydroxide aqueous solution to obtain degreasing fish scales;

[0049] S3 Soak the degreased fish scales obtained in step S2 in 10% hydrochloric acid aqueous solution for 3 hours, the quality of the hydrochloric acid aqueous solution is 6 times the mass of the degreased fish scales, and wash with distilled water until neutral to obtain pretreated fish scales;

[0050] S4 with step S3 gained pretreatment fish scale un...

Embodiment 3

[0057] Embodiment 3 A kind of method that extracts collagen from fish scale

[0058] The described method for extracting collagen from fish scales comprises the following steps:

[0059] S1 Add water 8 times the weight of fish scales to fish scales, heat to 45°C, soak for 3 hours to obtain rehydrated fish scales;

[0060] S2 Add the rehydrated fish scales obtained in step S1 into 12% sodium hydroxide aqueous solution and soak for 25 hours. The quality of the sodium hydroxide aqueous solution is 4 times the mass of the rehydrated fish scales. Remove the sodium hydroxide aqueous solution to obtain degreasing fish scales;

[0061] S3 Soak the degreased fish scales obtained in step S2 in 10% hydrochloric acid aqueous solution for 2 hours, the quality of the hydrochloric acid aqueous solution is 5 times the mass of the degreased fish scales, and wash with distilled water until neutral to obtain pretreated fish scales;

[0062] S4 with step S3 gained pretreatment fish scale under ...

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Abstract

The present invention belongs to the field of chemical industry and particularly relates to a method for extracting collagen from fish scales. The method for extracting the collagen from the fish scales comprises the following steps: firstly, after fish scales are rehydrated, a sodium hydroxide aqueous solution is added to remove fat, then acid is added for washing to neutrality to obtain pretreated fish scales, then the pretreated fish scales are irradiated, after crushing and enzymatic hydrolysis, a thiol protease inhibitor is added to obtain a crude collagen extract, then the sodium hydroxide aqueous solution is added to precipitate collagen, an acetic acid aqueous solution is added for dissolving, an ion exchange column is used for purification, and finally irradiating is conducted. The provided method for extracting the collagen from the fish scales can maximally maintain activity of the extracted collagen, at the same time can remove drug residues contained in the fish scales, improves stability of the collage and thus provides the safe and effective collagen.

Description

technical field [0001] The invention belongs to the field of chemical industry, in particular to a method for extracting collagen from fish scales. Background technique [0002] Fish scales are rich in protein and calcium, phosphorus and other minerals, mainly composed of protein and hydroxyapatite, of which protein accounts for 50% to 70% of the total weight of fish scales, mainly collagen and keratin. It can be seen that fish scales are very good Raw material for collagen production. There are a variety of amino acids in this type of collagen, which can improve the nutrition of human cells, promote the metabolism of skin and bone tissue, enhance skin elasticity, and make the skin smooth and delicate. Vitality has a good effect. However, the nutrients in fish scales have been forgotten by people. A large number of available fish scales are treated as garbage, which not only does not make full use of the value of fish scales, but also pollutes the environment. Therefore, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/30C07K1/18C07K1/36C07K1/14
Inventor 邹伟权
Owner 广州华大生物科技有限公司
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