Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of transferrin (trf) detection kit

A detection kit and transferrin technology, applied in the field of biochemical detection, can solve the problems of poor stability, non-resistance to heparin interference, low sensitivity, etc., and achieve enhanced stability, good accuracy and stability, and strong anti-interference Effect

Active Publication Date: 2021-02-26
BIOBASE BIODUSTRY (SHANDONG) CO LTD
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the object of the present invention is to provide a transferrin (TRF) detection kit, which adopts latex-enhanced immune turbidimetric method, overcomes the low sensitivity, poor stability, and no resistance to heparin interference of common immune turbidimetric method. Shortcoming, can effectively detect the content of transferrin (TRF), has the advantage of high sensitivity, good stability; Another object of the present invention is to provide the preparation and use method of this detection kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of transferrin (trf) detection kit
  • A kind of transferrin (trf) detection kit
  • A kind of transferrin (trf) detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The detection reagent of transferrin (TRF) provided by this embodiment includes reagent R1 and reagent R2:

[0025] The components of reagent R1 include:

[0026]

[0027] Described reagent R2 comprises the component of following content:

[0028]

[0029]

[0030] The usage method of this embodiment reagent:

[0031] The transferrin (TRF) detection kit described in this embodiment adopts a fully automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 fully automatic analyzer, etc., and uses the endpoint method for determination. Place R1 and R2 on the corresponding reagent positions according to the ratio of 4:1, and place distilled water, standards and samples on the corresponding positions of the sample plate. The operation is shown in Table 1.

[0032] Table 1 Reagent detection method table

[0033]

[0034] Calculation: transferrin (TRF) content (mg / dL) = (ΔA determination ÷ ΔA standard) × C standard

Embodiment 2

[0036] The detection reagent of the transferrin (TRF) described in this embodiment includes reagent R1 and reagent R2 (detection method is the same as embodiment 1):

[0037] The components of reagent R1 include:

[0038]

[0039]

[0040] Described reagent R2 comprises the component of following content:

[0041]

Embodiment 3

[0043] The detection reagent of the transferrin (TRF) described in this embodiment includes reagent R1 and reagent R2 (detection method is the same as embodiment 1):

[0044] The components of reagent R1 include:

[0045]

[0046] Described reagent R2 comprises the component of following content:

[0047]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to the technical field of transferrin (TRF) detection, in particular to a transferrin (TRF) detection kit and its preparation and use method. Reagent R1 contains buffer, lithium chloride, L(+)-mouse Lee sugar, γ-cyclodextrin, 1,2-propylene glycol, PEG-10000, rosin polyoxyethylene sucrose acrylate, preservatives; reagent R2 contains buffer, L(+)-rhamnose, γ-cyclodextrin Ethyl alcohol, 1,2-propylene glycol, rabbit anti-human transferrin (TRF) antibody coated latex particles, acrylic rosin polyoxyethylene sucrose ester, preservative; the present invention significantly improves the stability and linear range of the reagent, and significantly enhances the Reagent sensitivity, anti-heparin interference ability and accuracy.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and in particular relates to a transferrin (TRF) detection kit and a preparation and use method thereof. Background technique [0002] Transferrin, also known as transferrin (transferrin, TRF, siderophilin), has a molecular weight of about 77,000. It is a single-chain glycoprotein with a sugar content of about 6%. It is the main iron-containing protein in plasma and is responsible for carrying the Iron and iron released by degradation of red blood cells. TRF-Fe 3+ The complex form enters the bone marrow for the production of mature red blood cells. Transferrin (TRF) is mainly synthesized by hepatocytes with a half-life of 7 days. The concentration of TRF in plasma is regulated by iron supply. In the state of iron deficiency, the concentration of plasma transferrin (TRF) increases and returns to normal level after effective treatment. [0003] Transferrin (TRF) tends to decrease ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803
Inventor 甘宜梧李静李志明王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD