microbial flora and application thereof in treating Cr heavy metal pollution

A microbial flora, heavy metal technology, applied in biological water/sewage treatment, microorganism-based methods, microorganisms, etc., can solve problems such as strong toxicity

Inactive Publication Date: 2019-04-16
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this reduction process, Cr intermediates with a valence of pentavalent and active oxygen radicals or radical radicals will be produced, so they are highly toxic.

Method used

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  • microbial flora and application thereof in treating Cr heavy metal pollution
  • microbial flora and application thereof in treating Cr heavy metal pollution
  • microbial flora and application thereof in treating Cr heavy metal pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for enriching microbial flora, characterized in that it comprises the steps of:

[0023] (1) Collect the pond mud bottom as an environmental bacterial source, put the pond bottom mud into a culture bottle, use potassium dichromate to adjust the Cr(VI) concentration in the culture bottle, and set the volume to 600mL. After packaging, place it at 28°C Cultivate statically at high temperature for 30 days to obtain the first generation of enriched flora.

[0024] The physical and chemical properties of the pond bottom sludge are organic matter 30.15g / kg, total nitrogen 1.33g / kg, available phosphorus 35.4mg / kg, and water content 71%.

[0025] (2) Transfer the obtained first-generation enriched flora to fresh Cr(VI)-containing PCS culture medium, and culture at 28°C for 7 days to obtain the second-generation enriched flora. This method was transferred 5 times to obtain the 5th generation enriched bacterial population. The ability to transform Cr(VI) was stable, and...

Embodiment 2

[0033] The bacteria were cultured in the PCS culture medium containing Cr(VI), the initial pH of the culture medium was adjusted, and the ability of the bacteria to reduce Cr(VI) under different pH conditions was examined. The result is as figure 2 As shown, when pH=7 and pH=8, the flora can effectively reduce Cr(VI) in the culture solution. When the pH=7, cultured for 73 hours, the concentration of Cr(VI) in the culture medium decreased from 92.15μg / mL in the initial culture to 2.19μg / mL, and 97.6% of Cr(VI) was reductively transformed. When pH=8, after 73 hours of cultivation, the concentration of Cr(VI) in the culture medium was 0 μg / mL, and the reduction rate reached 100%.

Embodiment 3

[0035] Culture the flora in PCS culture solution containing 100 μg / mL Cr(VI), adjust the pH=8, and detect the change of flora OD600 during the culture process, the results are as follows image 3 . OD600 can reflect the biomass change in the process of microbial growth. Depend on image 3 it can be discovered. The flora can start to grow in the Cr(VI)-containing solution, and the OD reaches 0.41 after 24 hours of culture, and the biomass of the flora also increases with the increase of the culture time, and reaches 0.83OD at 92.5h.

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Abstract

The invention provides microbial florawhich is composed of bacteria and archaea. The bacteria (manipulation taxon, recorded as OTUs) include Burkholderiales, Clostridiales and other strains. The archaea (OTUs) include Methanobacteriales, Methanomicrobiales, Methanosarcinales and other strains. The microbial flora can effectively reduce Cr (VI) and can completely reduce 91 microgram/mL of Cr (VI) within 49.5 h. The microbial flora can tolerate the environmental Cr(VI) concentration of 435 microgram/mL and maintain the reduction capacity. The microbial flora has the capacity of reducing Cr (VI)at 15-45 DEG C, and the efficiency of reducing Cr(VI) at 30 DEG C and 35 DEG C is the best.

Description

technical field [0001] The invention relates to a microbial flora, and applies the microbial flora to environments polluted by heavy metals containing Cr, including water environments, soil environments, and organic polluted environments, and belongs to the field of biotechnology. Background technique [0002] Heavy metal pollution in my country is relatively serious. According to statistics, the area of ​​heavy metal pollution in my country's soil is about 2×10 7 hm 2 , the annual amount of contaminated grain is 1.2×10 7 t, economic loss up to 2×10 10 Yuan (Zhao Qiguo, et al., Acta Soil Science, 2007, 44(6): 1127-1134). [0003] Chromium is one of the main heavy metal pollutants. Because it is an important raw material in some industries such as electroplating, wood preservation, dyes, leather production, and alloy manufacturing, it is widely used in industrial production, and with the discharge of industrial wastewater, a large amount of it enters the environment (Dong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/22C12R1/01
CPCB09C1/10C02F3/34C02F2101/22C12N1/20
Inventor 吕育财杨涛龚大春张耀平李宁郭金玲田毅红祁正
Owner CHINA THREE GORGES UNIV
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