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Preparation and application of intelligent virus molecular imprinting resonance light sensor based on metal organic framework

A metal-organic framework and molecular imprinting technology, applied in the field of analytical chemical detection, can solve the problems of complexity and difficulty in elution and recombination of template virus molecules, achieving a simple construction process, conducive to preservation and long-term use, and low professional requirements. Effect

Active Publication Date: 2019-04-16
XIANGTAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the construction of viral molecular imprinted sensors using MOFs materials as imprinted carriers.
[0004] (2) With the existing molecular imprinting technology, the elution and recombination of the template virus molecules are relatively difficult and complicated

Method used

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  • Preparation and application of intelligent virus molecular imprinting resonance light sensor based on metal organic framework
  • Preparation and application of intelligent virus molecular imprinting resonance light sensor based on metal organic framework
  • Preparation and application of intelligent virus molecular imprinting resonance light sensor based on metal organic framework

Examples

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Effect test

Embodiment 1

[0030] Example 1: A preparation method of an intelligent virus molecularly imprinted resonance photosensor based on metal organic framework

[0031] (1) Preparation of metal-organic framework MIL-101: Cr(NO 3 ) 3 9H 2 O (2.00g), terephthalic acid (TPA, 0.82g) was added to 25mL of sodium acetate aqueous solution with a concentration of 0.05mol / L, and after magnetic stirring for 30min, the solution was transferred to a 50.0mL polytetrafluoroethylene reactor. Then the reaction kettle was sealed and placed in a constant temperature blast drying oven at 200°C. After 12 hours, the reactor was taken out, and after cooling to room temperature, the sample was fully washed with ultrapure water and filtered, and then dried in a constant temperature drying oven at 50°C for 5 hours to obtain dark green crystal MIL-101. Afterwards, add 0.2g MIL-101 and 10mL absolute ethanol to the plastic centrifuge tube, seal the whole system and place it in a water bath at 60°C, add 15mL of NH with a c...

Embodiment 2

[0034] Example 2: Verification of the feasibility of detecting viruses by the MIL-101@MIPs resonant light sensor

[0035] In order to verify the practicability of the present invention, this embodiment verifies the construction principle of the sensor. Such as figure 2 As shown, the comparison of the resonance light intensity at 470nm is: HAV+MIL-101@MIPs>MIL-101@MIPs>MIL-101>HAV. This shows that the MIL-101@MIPs sensor described in the present invention can well adsorb HAV particles, thereby causing a change in the particle size of the entire sensor, so that the resonant light scattering intensity is significantly enhanced.

Embodiment 3

[0036] Example 3: Characterization of the performance, morphology and structure of the MIL-101@MIPs resonant photosensor and intermediate products.

[0037] To verify the pH response performance of the MIL-101@MIPs sensor, we investigated the effect of different pH values ​​on the swelling rate of MIL-101@MIPs particles ( image 3 ): When the pH of the solution decreases from 9.0 to 2.0, the SR value increases slowly, which is due to the fact that the reagents forming the imprinted layer are cationic, and when the pH decreases, the polymer becomes more hydrophilic, which causes the polymer layer to swell .

[0038] Thermogravimetric experiments were used to evaluate the thermal stability of the MIL-101@MIPs resonant photosensor ( Figure 4 ). Such as Figure 4 It is shown that when the temperature is 50-100°C, the weight of the imprinted polymer decreases by 20.01%, obviously, this is caused by the disappearance of water molecules in its structure. While the weight loss in...

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Abstract

The invention discloses a preparation and application of an intelligent virus molecular imprinting resonance light sensor based on a metal organic framework. According to the preparation method, a metal organic framework material is used as a supporting body for the first time, and a pH sensitive polymer is an imprinting layer material, so that the molecular imprinting resonance light sensor for detecting viruses is prepared. Due to the fact that the specific surface area of the metal organic framework material is large, more binding sites can be provided; and meanwhile, the pH sensitive polymer has the property of intelligently responding to a pH value so that adsorption and elution of virus macromolecules can be realized more conveniently and more efficiently. Experimental results show that the sensor has good selectivity to template molecules and has a wide linear range, and the detection limit reaches 0.1 pM; the imprinted polymer can be recycled; and finally, the sensor is simpleto prepare, the detection process is simple and convenient to operate, powerful means for detection and diagnosis of the viruses are provided, and an important application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry detection, and in particular relates to the preparation and application of an intelligent virus molecular imprinted resonance optical sensor based on a metal organic framework. Background technique [0002] In recent years, molecular imprinting technology has developed rapidly in the field of virus detection. However, due to the large size of virus molecules (usually between 20 and 900 nm) [Shawky S.M., Awad A.M., Allam W., Alkordi M.H. , EL-Khamisy S.F., Biosens.Bioelectron., 2017, 92:349-356.], the application of this technology in the field of virus detection has been greatly limited. Later, the proposal of surface molecular imprinting technology provided a powerful means to break through this problem. Nevertheless, there are still some deficiencies in the current application of this technology in virus detection, which need to be resolved urgently. [0003] (1) The carrier mater...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/49
CPCG01N21/49G01N21/6402
Inventor 蔡昌群罗谅晖陈小明冯文宝
Owner XIANGTAN UNIV
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