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Application of a metal iridium complex nanoparticle in nuclide-excited phosphorescence imaging

A complex, metal iridium technology, applied in the direction of luminescent materials, indium organic compounds, platinum group organic compounds, etc., can solve the problems of inability to penetrate deep tissue, high energy, short wavelength, etc., to improve biocompatibility and optical sensitivity. High, good photostability

Active Publication Date: 2021-09-07
SHANGHAI NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because fluorescence imaging requires real-time excitation of external excitation light, and the excitation wavelength range of iridium complexes is mainly in the near-ultraviolet region, the wavelength is short and the energy is high, so it cannot penetrate deep tissues; at the same time, many substances in the body are affected by the in vivo imaging. After excitation, non-specific fluorescence will be generated, which will generate strong background noise during the imaging process, resulting in a low signal-to-noise ratio, which limits the application of iridium complexes in the field of in vivo fluorescence imaging

Method used

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  • Application of a metal iridium complex nanoparticle in nuclide-excited phosphorescence imaging
  • Application of a metal iridium complex nanoparticle in nuclide-excited phosphorescence imaging
  • Application of a metal iridium complex nanoparticle in nuclide-excited phosphorescence imaging

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Embodiment 1

[0030] 1. Preparation of metal iridium complex nanoparticles: dipalmitoyl phosphatidylcholine (DPPC), cholesterol, DSPE-PEG 2000 , the iridium complex whose structural formula is formula (I) is dissolved in chloroform according to the molar ratio of 6:2:0.5:0.5, and the solvent is removed by rotary evaporation at 30 to 40 °C after mixing evenly; 10 mL of water is then added to carry out hydration reaction, stirring at 45 °C The reaction was carried out for 0.5 hours. The cell crusher was sonicated for 10 min, and the power was 35%. After 0.22 μm filter membrane, dialysis in secondary water for 24 hours, iridium complex nanoparticles were formed.

[0031] 2. Fluorodeoxyglucose ( 18 F-FDG) was provided by the Department of Nuclear Medicine, Affiliated Cancer Hospital of Fudan University, at 100 μCi, the emissive excited phosphorescence of 60 μg / mL Ir-(I) nanoparticles was 18 F-FDG's Cherenkov luminous 3.8 times. For details, see figure 1 .

Embodiment 2

[0033] 18 The maximum wavelength of phosphorescence of F-FDG at 100 μCi with 60 μg / mL Ir-(I) nanoparticles (using the iridium complex nanoparticles prepared in Example 1) is located at 620 nm. For details, see figure 2 .

Embodiment 3

[0035] 1. Preparation of metal iridium (Ⅱ) complex nanoparticles: dipalmitoyl phosphatidyl choline (DPPC), cholesterol, DSPE-PEG 2000, the iridium complex whose structural formula is formula (II) is dissolved in chloroform according to the molar ratio of 6:2:0.5:0.5, and the solvent is removed by rotary evaporation at 30 to 40 °C after mixing evenly; then 10 mL of water is added for hydration reaction, and stirring at 45 °C The reaction was carried out for 0.5 hours. The cell crusher was sonicated for 10 min, and the power was 35%. After 0.22 μm filter membrane, dialysis in secondary water for 24 hours, iridium complex nanoparticles were formed.

[0036] 2. Take 100μL, 100μg / mL of iridium complex nanoparticles of formula (II), add 60, 150, 300, 450, 600μCi 18 The radioactive excitation phosphorescence intensity of F-FDG is the same dose, respectively 18 The Cherenkov luminescence of F-FDG is 1.3, 1.7, 2.1, 1.7, 1.6 times. Specifically as image 3 shown.

[0037] Figure...

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Abstract

The invention relates to the field of nanomaterials and molecular imaging, in particular to the application of a metal iridium complex solution mixed with a nuclide solution in phosphorescence imaging. The concentration of the metal iridium complex solution is 0.05-10 mg / mL. The activity of the nuclide solution is 0.01-10mCi. The present invention improves the biocompatibility of the metal iridium complex through the method of liposome encapsulation, so that it has good photostability, chemical stability and low cytotoxicity, and changes its original ability to be stimulated by an external excitation light source to produce strong The imaging method of phosphorescence with background noise expands its application in radionuclide-excited phosphorescence, and may have broad application prospects in the field of molecular imaging. The invention provides a new excitation method for metal iridium complex phosphorescence, which is applied in fluorescence imaging, can collect more optical signals, has high optical sensitivity, obtains better tissue penetration, and can detect deeper tissues that cannot detected optical signal.

Description

technical field [0001] The invention relates to the fields of nanomaterials and molecular imaging, in particular to the application of a metal iridium complex in nuclide-excited phosphorescence imaging. Background technique [0002] Cerenkov luminescence imaging (CLI) is a new optical imaging method based on the Cerenkov radiation (CR) effect of nuclides. CR means that when the speed of charged particles running in the medium is greater than the speed of light running in the medium, the charged particles can convert part of their energy into visible light, which can be collected by a high-sensitivity optical camera and then optically imaged. It is Cherenkov optical imaging. The spectrum is continuous and the peaks are mainly distributed in the ultraviolet and blue light bands. The radionuclides currently in use often produce high-energy charged particles such as β+, β- or α when they decay, including β-decay nuclides ( 131 I, 90 Y, 177 Lu), β+ decay nuclides ( 18 F, 6...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00C07F15/00C09K11/06
CPCA61K49/0021A61K49/0084C07F15/0033C09K11/06C09K2211/185
Inventor 杨红侯雨桐王晨晨周治国杨仕平
Owner SHANGHAI NORMAL UNIVERSITY
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