Mechanically-reinforced composite tissue engineering scaffold material and preparation method thereof
A technology of composite tissue and scaffold materials, applied in the field of biomedical materials, can solve problems such as excessive degradation, mismatch between degradation rate and tissue growth rate, aseptic inflammatory response, etc.
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Embodiment 1
[0054] Embodiment 1, the preparation of composite support material
[0055] (1) Evenly stretch the degummed mulberry silk into a mesh structure, cut it into a silk mesh with a length of 7cm, a width of 4cm, and a thickness of 0.5cm, with a mass of 30mg, and spread the silk mesh into a mold of 7cm×4cm×1cm Inside.
[0056] (2) 9 g of gelatin was dissolved in deionized water so that the concentration of the gelatin solution was 30%. 0 mg polylactic acid porous microspheres, 2.25 mL of silk fibroin protein solution with a concentration of 20 mg / mL and 1.5 mL of 30% gelatin solution were blended, and the volume of the resulting suspension was 15 mL, so that the concentration of porous microspheres was 0 mg / mL. The protein concentration is 3mg / mL, the gelatin concentration is 3wt%, and the gelatin mass in the suspension is 450mg.
[0057] (3) Inject the suspension in step (2) into the mold in step (1), cool and shape at -20°C, and vacuum freeze-dry (the freeze-drying temperature i...
Embodiment 2
[0059] Embodiment 2, the preparation of composite support material
[0060] (1) Evenly stretch the degummed mulberry silk into a mesh structure, cut it into a silk mesh with a length of 7cm, a width of 4cm, and a thickness of 0.5cm, with a mass of 45mg, and spread the silk mesh into a mold of 7cm×4cm×1cm Inside.
[0061] (2) 9 g of gelatin was dissolved in deionized water so that the concentration of the gelatin solution was 30%. 225mg of polylactic acid porous microspheres and 9mL concentration of 25mg / mL silk fibroin protein solution and 4.5mL30% gelatin solution are blended to obtain 15mL of suspension, so that the concentration of porous microspheres is 15mg / mL, the concentration of silk fibroin protein It is 15mg / mL, the gelatin concentration is 9wt%, and the gelatin quality in the suspension is 1350mg.
[0062] (3) Inject the suspension in step (2) into the mold in step (1), cool and shape at -20°C, and vacuum freeze-dry (the freeze-drying temperature is -55°C) to init...
Embodiment 3
[0064] Embodiment 3, the preparation of composite support material
[0065] (1) Evenly stretch the degummed tussah silk into a mesh structure, cut it into a silk mesh with a length of 7cm, a width of 4cm, and a thickness of 0.5cm, with a mass of 40mg, and spread the silk mesh into a mold of 7cm×4cm×1cm Inside.
[0066] (2) 9 g of gelatin was dissolved in deionized water so that the concentration of the gelatin solution was 30%. 160mg of polylactic acid porous microspheres were blended with 7.5mL of silk fibroin protein solution with a concentration of 20mg / mL and 3.5mL of 30% gelatin solution to obtain a suspension of 15mL, so that the concentration of porous microspheres was 10.67mg / mL. The protein concentration is 10mg / mL, the gelatin concentration is 7wt%, and the mass of gelatin in the suspension is 1050mg.
[0067] (3) Inject the suspension in step (2) into the mold in step (1), cool and shape at -20°C, and vacuum freeze-dry (the freeze-drying temperature is -55°C) to i...
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