Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial exopolysaccharide capable of condensing petroleum

A technology of extracellular polysaccharides and microorganisms, applied in the field of oil pollution control, can solve pollution and other problems, and achieve the effect of good application prospects and high coagulation and degradation efficiency

Active Publication Date: 2019-05-03
DALIAN NATIONALITIES UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microbial treatment of environmental oil pollution reported in the literature mainly focuses on the use of microorganisms to degrade oil. Aiming at the problem of rapid diffusion of pollution from floating oil films in the ocean, there is no report on the use of microbial metabolites to carry out bio-coagulation recovery treatment of polluted oil films. The present invention not only solves the problem of pollution problem, and will also provide a new method for the recovery and reuse of polluted oil

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial exopolysaccharide capable of condensing petroleum
  • Microbial exopolysaccharide capable of condensing petroleum
  • Microbial exopolysaccharide capable of condensing petroleum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this example, exopolysaccharides were extracted by using bacterial strain CS07, which is named Marinobacter maritimus in Latin literature. Classification name: Marinobacter maritimus. The strain CS07 is collected from seabed sediments in Dalian Xingang oil-polluted sea area, enriched and isolated. The described strain CS07 has been submitted for deposit, and the specific deposit information is as follows:

[0023] Name of depository unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC);

[0024] Address of Preservation Unit: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing;

[0025] Deposit date: July 6, 2016;

[0026] Deposit number: CGMCC No.12739;

[0027] The morphological and physical and chemical characteristics of the strain CS07 are as follows: the strain CS07 was streaked and cultured on a solid LB medium at 15°C to a single colony, and ...

Embodiment 2

[0049] The component analysis of the exopolysaccharide obtained in Example 1 was carried out. EPS1-1(6) was placed in a screw sample bottle, 1ml of 2mol / L trifluoroacetic acid was added, the bottle was tightened, and hydrolyzed at 110°C for 10h. The hydrolyzed sample was concentrated by vacuum rotation at 60°C, and then dissolved in 500ul sterile water for later use. Adopt thin layer chromatography (TLC) method and high performance liquid chromatography (HPLC) method to analyze its component:

[0050] 1. TLC

[0051] ①Sampling: Take 10ul samples and place samples at about 1.5cm below the 10×20CM microcrystalline cellulose plate, with 5mg / L fructose, xylose, glucose, galactose, arabinose, rhamnose, and mannose as the standard Sample, the standard sample sample volume is 2.5ul.

[0052] ②Expansion layer: Put the microcrystalline cellulose plate with the sample in the layer development cylinder, use ethyl acetate: pyridine: glacial acetic acid: water = 8:5:1:1.5 (V / V) as the la...

Embodiment 3

[0074] Adopt high performance gel permeation chromatography to measure the weight average molecular weight of EPS1-1 (6), the high performance gel permeation chromatography measurement condition is as follows:

[0075] Column: Waters Ultrahydrogel TM 500(7.8×300mm, 5μm); flow rate: 1mL / min; mobile phase: Watsons distilled water; column temperature: room temperature; injection volume: 70μL; acquisition time: 60min; detector: laser detector (LS), differential light detection device (RI).

[0076] The results showed that the weight average molecular weight (Mw) of EPS1-1(6) was 26496g / mol.

[0077] Table 4 The absolute molecular weight determination results of EPS1-1(6)

[0078]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Weight average molecular weightaaaaaaaaaa
Weight average molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of petroleum pollution treatment, and specifically relates to a microbial exopolysaccharide capable of condensing petroleum. The microbial exopolysaccharide has a weight average molecular weight of 26469 g / mol and is composed of two monosaccharides namely L-arabinose and D-mannose according to a molar ratio of 1.19: 5.33. The microbial exopolysaccharide providedby the invention has the function of condensing the petroleum, can condense the petroleum into a spherical shape, has high condensation and degradation efficiency and good application prospect, and lays a foundation for further exploration of petroleum condensation and degradation products.

Description

technical field [0001] The invention belongs to the field of oil pollution control, in particular to a microbial extracellular polysaccharide capable of condensing oil. Background technique [0002] With the aggravation of oil pollution, the research on oil degradation has attracted more and more attention. In particular, oil pollution occurs in water bodies, including oil spills, oil port wharf ships, and land oil entering the ocean or other water bodies. Due to the low density of oil, it can float on the surface of water bodies and expand rapidly with the flow of water bodies, resulting in rapid expansion of the polluted area. . At present, most of the microbial treatment of environmental oil pollution reported in the literature mainly focuses on the use of microorganisms to degrade oil. Aiming at the problem of rapid diffusion of pollution from floating oil films in the ocean, there is no report on the use of microbial metabolites to carry out bio-coagulation recovery tr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00C02F3/34C02F103/36
Inventor 刘秋袁烽皓于基成陈超
Owner DALIAN NATIONALITIES UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com