Lateral flow biosensor based on gold platinum nanoflower
A flow biology, platinum nanotechnology, applied in immunoassay and other biological detection fields, can solve the problems of inability to detect protein biomarkers, limited surface area of gold nanoparticles, difficult preparation of nanomaterial labels, etc. Anti-interference, good effect of peak signal
Inactive Publication Date: 2019-05-07
ANHUI SCI & TECH UNIV
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Problems solved by technology
However, AuNP-based lateral flow immunoassays cannot detect certain protein biomarkers at low concentrations in physiological fluids due to the limited surface area of AuNPs
Other nanoparticle tags associated with highly sensitive sensors can indeed greatly improve the sensitivity of lateral flow immunoassays, but unfortunately these quantitative methods are dependent on complex signal readout instruments and these nanomaterial tags are difficult to prepare
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[0023] The present invention will be further described below in conjunction with the accompanying drawings.
[0024] The sources of reagents used in the implementation are as follows: chloroauric acid tetrahydrate (HAuCl 4 .4H2O) were purchased from Bailingwei Technology Co., Ltd. (Beijing, China); 3-amino-9-ethyl-carbazole (AEC), hydrogen peroxide solution (H 2 o 2 ), ethanolamine, bovine serum albumin (BSA), sucrose, Tween 20, rabbit IgG, phosphate buffered saline (0.1M PBS, pH 7.4) were purchased from Shanghai Sangon Bioengineering Co., Ltd. (Shanghai, China); goat antibody Rabbit IgG (GaR IgG, Ab1) and donkey anti-sheep IgG (Ab2) were purchased from Beijing Boao Biological Co., Ltd. (Beijing, China). Glass fiber sample pads, polyvinyl chloride (PVC) adhesive plastic-backed plates, cellulose fibers and nitrocellulose membranes (Pall 90) were all purchased from Shanghai Jiening Biotechnology Co., Ltd. (Shanghai, China).
[0025] Some experimental instruments and their use...
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Abstract
The invention belongs to the technical field of immunoassay and other biological detection, and in particular relates to a lateral flow biosensor based on a gold platinum nanoflower. The gold platinumnanoflower is a nano-composite material obtained by growing a platinum nanowire on the surface of gold nanoparticles; a gold platinum nanoflower-antibody 1 mixed solution is prepared by taking the gold platinum nanoflower as the raw material, and mixed with a protein target; then, the protein target is detected by the lateral flow biosensor; and the protein target is the rabbit immunoglobulin. Compared with the prior art, the lateral flow biosensor based on the gold platinum nanoflower in the invention has the advantages that: the gold platinum nanoflower-antibody 1 mixed solution is preparedbased on the gold platinum nanoflower, and used for the prepared lateral flow biosensor; the target protein having relatively low concentration can be detected; the anti-interference performance is excellent; quantitative detection is carried out by measuring the peak area of a test strip through a portable strip reader; and thus, the lateral flow biosensor is good in peak signal, high in sensitivity, simple to operate, good in specificity, and suitable for popularization and use.
Description
technical field [0001] The invention belongs to the technical field of immunoassay and other biological detection, and in particular relates to a lateral flow biosensor based on gold-platinum nanoflowers. Background technique [0002] Proteins are the material basis of life and play key roles in biological processes. The detection of protein targets has attracted considerable interest in various research fields, including clinical diagnostics, therapeutic monitoring, food safety, and environmental analysis. Techniques commonly used to detect proteins include silver staining, agarose and polyacrylamide gel electrophoresis, radioimmunoassay, enzyme-linked immunosorbent assay (ELISA), western blotting, and mass spectrometry. However, these techniques are time- and cost-intensive and limited to laboratory use due to the need for sophisticated instrumentation and highly trained personnel. Therefore, it is still necessary and very important to develop easy-to-operate and simple ...
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Login to View More IPC IPC(8): G01N33/543G01N33/558G01N33/535
Inventor 刘国东于庆才张静钱立生张学记
Owner ANHUI SCI & TECH UNIV