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Preparing method of oxygen-generation nano-liposome

A nano-liposome and product technology, which is applied in liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of poor safety performance and low stability, and achieve High performance stability, high repeatability, easy to promote the effect of application

Inactive Publication Date: 2019-05-10
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The oxygen-producing nanoliposomes prepared by the existing research have poor safety performance and low stability, and cannot meet the needs of improving the curative effect of malignant solid tumors, and need to be further improved.

Method used

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  • Preparing method of oxygen-generation nano-liposome
  • Preparing method of oxygen-generation nano-liposome

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A preparation method of oxygen-producing nanoliposomes, comprising the following steps:

[0030] (1) Add soybean lecithin to cyclohexane, vortex, and mix under ultrasonic wave for 4 minutes to obtain a mixed solution. The amount of soybean lecithin added to cyclohexane is 10 mg per milliliter;

[0031] (2) Add 20 mg / ml pectin solution to the mixture, stir for 35 minutes, and mix under ultrasonic waves for 18 minutes to obtain a suspension. The volume ratio of the pectin solution to cyclohexane in step (1) is 0.8 :100;

[0032] (3) Add 10 mg / ml sodium percarbonate solution to the suspension, vortex, and ultrasonically mix at 10°C for 18 minutes to obtain a mixed stirring liquid, the sodium percarbonate solution and cyclohexane in step (1) The volume ratio of alkane is 0.8:100;

[0033] (4) Add 1.4mg / ml calcium hydroxide solution to the mixing solution, stir at 10°C for 8 minutes, and ultrasonically mix at 10°C for 18 minutes to obtain a calcium hydroxide solution mixtu...

Embodiment 2

[0039] A preparation method of oxygen-producing nanoliposomes, comprising the following steps:

[0040] (1) Add soybean lecithin to chloroform, vortex, and mix under ultrasonic waves for 6 minutes to obtain a mixed solution. The amount of soybean lecithin added per milliliter of chloroform is 15 mg;

[0041] (2) Add 30 mg / ml pectin solution to the mixture, stir for 45 minutes, and mix under ultrasonic waves for 25 minutes to obtain a suspension. The volume ratio of the pectin solution to the chloroform in step (1) is 1.2: 100;

[0042] (3) Add 20 mg / ml sodium percarbonate solution to the suspension, vortex, and ultrasonically mix at 15°C for 25 minutes to obtain a mixed stirring liquid, the mixture of the sodium percarbonate solution and the chloroform in step (1) The volume ratio is 1.2:100;

[0043](4) Add 1.8mg / ml calcium hydroxide solution to the mixing solution, stir at 15°C for 12 minutes, and ultrasonically mix at 15°C for 25 minutes to obtain a calcium hydroxide solu...

Embodiment 3

[0049] A preparation method of oxygen-producing nanoliposomes, comprising the following steps:

[0050] (1) Add soybean lecithin to the mixed solvent of chloroform and cyclohexane, vortex, and mix under ultrasonic waves for 5 minutes to obtain a mixed solution. The amount of soybean lecithin added per milliliter of mixed solvent of chloroform and cyclohexane is 25mg, the volume ratio of chloroform and cyclohexane in the mixed solvent of chloroform and cyclohexane is chloroform: cyclohexane=1:1;

[0051] (2) Add 25 mg / ml pectin solution to the mixture, stir for 40 minutes, and mix under ultrasonic waves for 20 minutes to obtain a suspension. The mixture of the pectin solution and chloroform and cyclohexane in step (1) The volume ratio of the solvent is 1:100;

[0052] (3) Add 15 mg / ml sodium percarbonate solution to the suspension, vortex, and ultrasonically mix at 12°C for 20 minutes to obtain a mixed stirring liquid, the sodium percarbonate solution and the chloroform in ste...

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Abstract

The invention discloses a preparing method of an oxygen-generation nano-liposome. The method includes the steps of adding soybean lecithin and a pectin solution to a non-polarity organic solvent, making a sodium percarbonate solution and a calcium hydroxide solution react at a low temperature to generate peroxides, making the calcium hydroxide solution and the pectin solution have a crosslinking reaction, wrapping the peroxides by a calcium pectinate gel film, conducting vortex ultrasonic mixing and reverse-phase evaporating in a lecithin-non-polarity organic solvent, and adding water for vortex ultrasonic processing to prepare the oxygen-generation liposome. The prepared oxygen-generation nano-liposome is high in oxygen yield, can be stored for a long time under the freezing conditions, is high in performance stability and has the effective oxygen releasing time up to 55-60 h at the room temperature.

Description

technical field [0001] The invention relates to the technical field of liposome preparation, in particular to a preparation method of oxygen-producing nano-liposome. Background technique [0002] Liposome refers to the ultra-miniature spherical drug carrier preparation made by encapsulating drugs in the middle of the film formed by lipid bilayers. Because its structure is similar to biological membranes, it can encapsulate water-soluble and fat-soluble drugs, and has the ability to reduce Drug dose, reduce toxicity, delay release, change drug distribution in vivo, and have targeted therapeutic effect, improve drug efficacy. [0003] Tumor treatment is an unsolved problem today and a major challenge that the medical community has been facing. Drug therapy is an important means of tumor treatment. Although traditional anti-tumor drugs have achieved certain curative effects, their allergic reactions, inhibition of bone marrow hematopoietic system, hair loss and other toxic and...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/24A61K47/36A61K47/02A61K33/00A61P35/00A61P35/04
CPCA61K9/1271A61K9/1277A61K33/00A61K47/02A61K47/24A61K47/36A61P35/00A61P35/04
Inventor 聂国朝张军华王友东罗平
Owner YULIN NORMAL UNIVERSITY
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