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A method for preparing nanometer metal oxides using bacterial supernatant and its products and applications

A technology of oxides and metals, which is applied in the field of preparing nano-metal oxides, can solve the problems of chemical reagent environment and human health hazards, high equipment requirements, etc., and achieve good biocompatibility, simple preparation process, and small particle size.

Active Publication Date: 2021-08-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Therefore, the green synthesis technology of eco-friendly and biocompatible nano-metal oxides and its applicable products will become a research hotspot. The traditional preparation of nano-metal bacteriostatic agents is mainly through physical and chemical methods, but physical methods require a lot of equipment. High, and the chemical reagents used in chemical methods are harmful to the environment and human health

Method used

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  • A method for preparing nanometer metal oxides using bacterial supernatant and its products and applications
  • A method for preparing nanometer metal oxides using bacterial supernatant and its products and applications
  • A method for preparing nanometer metal oxides using bacterial supernatant and its products and applications

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Embodiment 1

[0037] Embodiment 1: Utilize bacterial supernatant to synthesize nanometer ZnO

[0038](1) Pick a single colony of Bacillus polymyxa and inoculate it in a 5ml LB liquid medium test tube. After cultivating overnight at 30°C and 200rpm / min, transfer the obtained bacterial liquid to 100ml LB liquid culture according to the inoculum amount of 1%. Medium, cultivate overnight at 30°C, 200rpm / min to reach a certain turbidity;

[0039] (2) Centrifuge the bacteria obtained from the overnight culture at a speed of 6000 rcf / min for 4 minutes, collect the supernatant and filter it with a water-based disposable needle filter with a diameter of 13 mm and a pore size of 0.22 μm to obtain a cell-free supernatant;

[0040] (3) Fully mix the obtained supernatant with the ZnO solution with a concentration of 0.5mM according to the volume ratio of 1:1, and then centrifuge at room temperature for 24h at a speed of 8000rcf / min for 10min. Washed twice with distilled water, and prepared into powdery...

Embodiment 2

[0046] Embodiment 2: Utilize bacterial supernatant to synthesize nanometer MgO

[0047] (1) Pick a single colony of Bacillus polymyxa and inoculate it in a 5ml LB liquid medium test tube. After cultivating overnight at 30°C and 200rpm / min, transfer the obtained bacterial liquid to 100ml LB liquid culture according to the inoculum amount of 1%. Medium, cultivate overnight at 30°C, 200rpm / min to reach a certain turbidity;

[0048] (2) Centrifuge the bacteria obtained from the overnight culture at a speed of 7000 rcf / min for 5 minutes, collect the supernatant and filter it with a water-based disposable needle filter with a diameter of 13 mm and a pore size of 0.22 μm to obtain a cell-free supernatant;

[0049] (3) Fully mix the obtained supernatant with 0.5mM methionine at a volume ratio of 2:1, and centrifuge at room temperature for 24 hours at a speed of 9000 rcf / min for 15 minutes, and wash the precipitate with distilled water Twice, the vacuum freeze-drying method was used t...

Embodiment 3

[0055] Embodiment 3: Utilize bacterial supernatant to synthesize nanometer MnO 2

[0056] (1) Pick a single colony of Bacillus polymyxa and inoculate it in a 5ml LB liquid medium test tube. After cultivating overnight at 30°C and 200rpm / min, transfer the obtained bacterial liquid to 100ml LB liquid culture according to the inoculation amount of 1%. Medium, cultivate overnight at 30°C, 200rpm / min to reach a certain turbidity;

[0057] (2) Centrifuge the bacteria obtained from the overnight culture at a speed of 6000 rcf / min for 4 minutes, collect the supernatant and filter it with a water-based disposable needle filter with a diameter of 13 mm and a pore size of 0.22 μm to obtain a cell-free supernatant;

[0058] (3) The resulting supernatant was mixed with MnO at a concentration of 0.5mM 2 The solution was fully mixed according to the volume ratio of 2:1, and then centrifuged at room temperature for 24 hours at a speed of 10,000 rcf / min for 20 minutes, the precipitate was wa...

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Abstract

The invention discloses a method for preparing metal oxide nanomaterials by using bacterial supernatant, which comprises the following steps: 1) culturing Paenibacillus polymyxa in LB liquid medium to obtain bacterial culture solution, centrifuging to obtain the supernatant, and passing through Filter to obtain the cell-free supernatant; 2) Mix the obtained cell-free supernatant with the metal oxide solution, let it stand at room temperature for 22-24 hours, centrifuge, wash, and vacuum freeze-dry to obtain the metal oxide nanomaterial; the metal oxide The substances are ZnO, MgO, MnO 2 any of the. The synthesis method of the method of the present invention is simple and rapid, green and environmentally friendly, and low in cost. The obtained nano-products have small particle size, good dispersibility, and good antibacterial effect, especially the ability to inhibit rice bacterial blight is very good, and can be used as a method for preventing and treating rice. Antibacterial agent for important plant diseases such as bacterial blight.

Description

technical field [0001] The invention relates to the field of biosynthetic nanomaterials, in particular to a method for preparing nanometer metal oxides using bacillus supernatant and an application thereof. Background technique [0002] Bacterial leaf blight is one of the most destructive rice diseases in the world's rice-growing areas, and it can cause up to 50% of commercial crop losses. At present, although chemical control methods play an important role in controlling the occurrence of diseases and ensuring production, they have potential harm to the environment, consumers and the development of bacterial resistance. Metals currently used as metal biocides include only Cu, Ag, Zn. According to previous reports, as a broad-spectrum bactericidal material, nano-ZnO can effectively kill Escherichia coli and Staphylococcus aureus, and has a strong antibacterial effect on Gram-positive and Gram-negative bacteria, and is widely used in the medical field. , coatings, packaging...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00A01N59/06A01N59/16A01P1/00C12R1/12
Inventor 李斌洪纤纤张牧晨刘柳邱文张阳索拉·奥莱坦
Owner ZHEJIANG UNIV
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