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A Visual Paper Sensing Method for Detecting Dopamine

A dopamine and sensing substrate technology, applied in the controllable preparation of nano-porphyrin, based on CdTe quantum dot paper sensing and detection of dopamine, can solve the problems of complex operation, inconvenient on-site detection, expensive equipment, etc., and achieve sensitivity and selectivity High, fast on-site detection, low cost effect

Active Publication Date: 2021-03-23
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of them require expensive equipment and complicated operations
And it can only be operated by well-trained professionals, and it is inconvenient to carry out on-site inspection

Method used

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  • A Visual Paper Sensing Method for Detecting Dopamine
  • A Visual Paper Sensing Method for Detecting Dopamine
  • A Visual Paper Sensing Method for Detecting Dopamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method for detecting dopamine in pure water based on CdTe quantum dot paper sensing substrate, comprising steps:

[0037] (1) Synthesis of CdTe quantum dots;

[0038] (1) Synthesis of CdTe quantum dots

[0039] Cadmium dichloride (0.098g) and N-acetyl-L-cysteine ​​(0.082g) were dissolved in 40mL of ultrapure water, and after stirring for 15 minutes at normal temperature and pressure, the pH of the solution was adjusted with sodium hydroxide solution. Adjusted to 8.85, then filled with nitrogen and stirred in an ice bath for 20 minutes, added sodium tellurite (0.0225g, 2.5mM), stirred for 15 minutes; then added sodium borohydride (0.0113g), stirred for 15 minutes, and finally put this solution into In the reaction kettle, react in an oven at 200°C for 50 minutes, and after cooling to room temperature, the emission wavelength is 630nm, red light is emitted, and the concentration is 8.032×10 -6 mol L -1 CdTe quantum dots.

[0040] (2) Synthesis of tetrakis-(4-pyridyl...

Embodiment 2

[0055] A method for detecting dopamine in a human plasma sample based on a CdTe quantum dot paper sensing substrate, comprising steps:

[0056] (1) Synthesis of CdTe quantum dots;

[0057] Adopt the method synthetic CdTe quantum dot of step (1) in embodiment 1

[0058] (2) Synthesis of tetrakis-(4-pyridyl)zinc self-assembled porphyrin

[0059] Four-(4-pyridyl) zinc nanoporphyrin solution was synthesized by the method of step (2) in Example 1.

[0060] (3) Preparation of CdTe quantum dot paper sensing substrate

[0061] The CdTe paper sensor substrate was prepared by the method of step (3) in Example 1.

[0062] (4) Preparation of human plasma sample solution

[0063] Human plasma was centrifuged and the supernatant was diluted 500 times as a solvent to prepare different concentrations of dopamine

[0064] (5) Make dopamine standard color card in human plasma sample

[0065] Preparation solution 1: a mixed solution of 1 nM dopamine human plasma sample and 15.28 μM tetraki...

Embodiment 3

[0073] The method for detecting dopamine in Duyun Maojian tea water sample based on CdTe quantum dot paper sensing substrate, comprising steps:

[0074] (1) Synthesis of CdTe quantum dots;

[0075] Adopt the method synthetic CdTe quantum dot of step (1) in embodiment 1

[0076] (2) Synthesis of tetrakis-(4-pyridyl)zinc self-assembled porphyrin

[0077] Four-(4-pyridyl) zinc nanoporphyrin solution was synthesized by the method of step (2) in Example 1.

[0078] (3) Preparation of CdTe quantum dot paper sensing substrate

[0079] The CdTe paper sensor substrate was prepared by the method of step (3) in Example 1.

[0080] (4) Preparation of dopamine Duyun Maojian tea water sample solution

[0081] Weigh 81mg of Duyun Maojian tea leaves, add 9mL of ultra-pure water and soak at 80°C for 10min, dilute the filtrate 200 times to make the tea water concentration 45mg·L -1 Prepare different concentrations of dopamine as a solvent

[0082] (5) Make standard color card of dopamine ...

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Abstract

The invention discloses a method for visual paper sensing detection of dopamine, and belongs to the technical field of nanomaterial preparation and chemical analysis and detection. Based on a CdTe quantum dot paper sensing substrate, different concentrations of dopamine and different concentrations of nano porphyrin react with the CdTe quantum dot paper sensing substrate to display different colors, so that the different concentrations of dopamine are detected. The method has the characteristics of easy preparation, fast field detection, low cost, high response speed, high sensitivity and highselectivity, and the CdTe quantum dot paper sensing substrate has a specific reaction to the dopamine, and can be used for detection of the dopamine in complex substrate samples.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and chemical analysis and detection, and in particular relates to a controllable preparation of nanoporphyrin and a method for sensing and detecting dopamine based on CdTe quantum dot paper. Background technique [0002] Dopamine is one of the most important neurotransmitters in the central and peripheral nervous systems, and its concentration disturbance is associated with a series of neurological diseases such as Parkinson's disease and Alzheimer's disease. Therefore, the development of simple, preferably low-cost and high-sensitivity methods for the detection of dopamine is of significant importance. So far, a variety of analytical methods, including electrochemical, chemiluminescence, high-performance liquid chromatography, capillary electrophoresis, and spectroscopic methods, have been successfully applied to the determination of dopamine. However, most of them require expen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 付海燕郭晓明佘远斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES