Method for quantitatively determining flavonoid compounds

A technology for quantitative determination of flavonoids, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of inconvenient on-site detection, high cost, and complexity, and achieve high sensitivity, fast response speed, and good stability.

Active Publication Date: 2019-05-10
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have high precision, high sensitivity, and low cross-interference, the experimental process requires complicated sampl

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  • Method for quantitatively determining flavonoid compounds
  • Method for quantitatively determining flavonoid compounds
  • Method for quantitatively determining flavonoid compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: The recognition and quantitative analysis of quercetin by the reversible nano-porphyrin fluorescent sensor, the schematic diagram of the method is shown in 1, and the steps are as follows:

[0044] (1) Synthesis of ZnCdSe quantum dot fluorescent probe

[0045] Dissolve zinc dichloride (0.035g, 6.4mM) and N-acetyl-L-cysteine ​​(0.1253g, 19.2mM) in 40mL of ultrapure water, stir for 20 minutes in an ice bath, and use Adjust the pH of the solution to 9.7 with sodium hydroxide solution, adjust the pH by adding 100 μL of cadmium dichloride (0.00058 g, 0.237 mM), then fill with nitrogen and stir in an ice bath for 5 to 10 minutes. NaHSe was added and stirred for 5 minutes. Finally, the solution was put into a reaction kettle and reacted in an oven at 200° C. for 65 minutes. Cooling to room temperature yielded 4.9×10 -9 mol / LZnCdSe quantum dot fluorescent probe.

[0046] (2) Synthesis of nanoporphyrin solution

[0047] Dissolve an appropriate amount of tetrak...

Embodiment 2

[0054] Embodiment 2: Quantitative analysis of rutin by a reversible nanoporphyrin fluorescent sensor, the schematic diagram of the method is shown in 1, and the steps are as follows:

[0055] (1) Synthesis of ZnCdSe quantum dot fluorescent probe

[0056] The ZnCdSe quantum dot fluorescent probe was synthesized by the method of step (1) in Example 1.

[0057] (2) Synthesis of tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution

[0058] The method of step (2) in Example 1 was used to synthesize tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution.

[0059] (3) Preparation of switch nanoporphyrin fluorescent sensor

[0060] The nano-porphyrin fluorescence sensor was prepared by the method of step (3) in Example 1.

[0061] (4) Quantitative analysis of rutin with reversible nanoporphyrin fluorescent sensor

[0062] Add 100 μL rutin aqueous solution to 1.5mL cuvette, 300 μL 1.68×10 -5mol / L tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution synthesized in step...

Embodiment 3

[0063] Embodiment 3: Quantitative analysis of kaempferol by reversible nanoporphyrin fluorescent sensor, the schematic diagram of the method is shown in 1, and the steps are as follows:

[0064] (1) Synthesis of ZnCdSe quantum dot fluorescent probe

[0065] The ZnCdSe quantum dot fluorescent probe was synthesized by the method of step (1) in Example 1.

[0066] (2) Synthesis of tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution

[0067] The method of step (2) in Example 1 was used to synthesize tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution.

[0068] (3) Preparation of switch nanoporphyrin fluorescent sensor

[0069] The nano-porphyrin fluorescence sensor was prepared by the method of step (3) in Example 1.

[0070] (4) Quantitative analysis of kaempferol by reversible nanoporphyrin fluorescent sensor

[0071] Add 100 μL kaempferol aqueous solution to 1.5mL cuvette, 300 μL 1.68×10 -5 mol / L tetrakis-(4-pyridyl)zinc porphyrin self-assembly solution synthes...

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Abstract

The invention discloses a method for quantitatively determining flavonoid compounds and belongs to the technical field of nanometer material preparation and chemical analysis and detection. Accordingto the method disclosed by the invention, a self-assembled nanocrystallized quadri-(4-pyridyl) zinc porphyrin photosensitive effect and a ZnCdSe quantum dot are prepared in a buffer solution system toobtain a reversible nanometer-porphyrin fluorescent sensor with a double-composite nanometer effect; then, a strong action force between the flavonoid compounds and the nanometer porphyrin is utilized to pull out specific changes of a reversible composite sensing interface between the nanometer porphyrin and the quantum dot to different degrees so as to realize quantitative detection of the flavonoid compounds. The preparation method disclosed by the invention is simple and controllable, and the flavonoid compound detection method is high in sensitivity, good in selectivity and high in specificity. The method not only can play an important role in flavonoid compound detection but also expectedly has an important application value in the fields of biochemistry and the like.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and chemical analysis and detection, and in particular relates to a controllable preparation of a novel reversible nanoporphyrin fluorescence sensor and a method for highly sensitive detection of flavonoids. Background technique [0002] Flavonoids are the products of plant secondary metabolism, which are widely found in various foods and beverages, such as tea, coffee, wine, fruits, vegetables and whole grains, and have obvious pharmacological activities, such as antioxidant , anti-inflammatory, anti-viral, etc. So far, a variety of analytical methods, including electrochemical high-performance liquid chromatography, capillary electrophoresis, electrospray mass spectrometry, high-speed countercurrent chromatography, and diode arrays, have been used to detect polyphenols. Although these methods have high precision, high sensitivity, and low cross-interference, the experimental pr...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 付海燕郭晓明佘远斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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