All-fiber STED super-resolution microscopic illumination device

A lighting device and super-resolution technology, applied in the field of fiber optics and optical microscopes, can solve problems such as low stability, complex optical paths, and complex optical paths, and achieve the effect of improving flexibility and integration

Active Publication Date: 2019-05-14
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

For example, the Chinese patent CN211817464U proposes a super-resolution fluorescence microscopy device based on tangential polarization. Although a good microscopic illumination effect is obtained, the realization of this effect requires complex optical paths, expensive precision instruments, and the stability is not very high.
Chinese patent CN103617330A proposes a super-resolution STED microscopic imaging device excited by a broadband laser light source generated by a supercontinuum. Although the function of synchronous adjustment of the two lasers is improved to achieve good microscopic illumination, the overall optical path is complicated

Method used

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  • All-fiber STED super-resolution microscopic illumination device
  • All-fiber STED super-resolution microscopic illumination device
  • All-fiber STED super-resolution microscopic illumination device

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Embodiment Construction

[0016] Below in conjunction with embodiment the present invention is described in more detail:

[0017] combine Figure 1-Figure 2 , the laser 1 simultaneously emits the short-wave laser 6 and the long-wave laser 7 through the short-wave laser 101 and the long-wave laser 102 respectively. On the one hand, the short-wavelength laser light 6 is collimated by the lens 12 and reflected by the half-mirror 13 , collected by the lens 14 and injected into the double-clad optical fiber 4 . Such as figure 2 As shown in (a), the injected short-wavelength laser 6 is transmitted in a single mode in the waveguide structure formed by the core 401 and the inner cladding 402, and finally is output from the end of the fiber in the form of Gaussian excitation light 8, figure 2 (c) and (f) show the light field distribution diagrams of the output excitation light; on the other hand, the long-wave laser 7 passes through the delay unit 2 after a delay of Δτ, collimates through the lens 15 and is r...

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Abstract

The invention provides an all-fiber STED super-resolution microscopic illumination device. The device mainly consists of a laser 1, a delay unit 2 and a beam generator 3, wherein the beam generator 3mainly consists of a double-clad fiber 4 and a polarization controller 5; the beam generator 3 can convert short-wave laser light 6 and long-wave laser light 7 emitted by the laser 1 into Gauss-shapedexcitation light 8 and hollow annular loss light 9, respectively; and since the light spot centers of the generated light are completely coincided and the light is irradiated on a fluorescent sample10 in sequence after the delay of the delay unit 2, stimulated emission depletion (STED) is generated, fluorescent light 11 with extremely small light spot is stimulated, and the final resolution is not limited by the diffraction of light, thereby breaking the diffraction limit and realizing all-fiber STED super-resolution microscopic illumination. The device has the advantages of highly integrating light beam generators and enabling equipment to be more flexible and stable.

Description

(1) Technical field [0001] The invention relates to the fields of fiber optic technology and optical microscope technology, in particular to an all-fiber stimulated emission depletion (STED) super-resolution microscopic illumination imaging device. (2) Background technology [0002] The development of modern biology and materials science has put forward higher and higher resolution requirements for microstructure research. Stimulated emission depletion (STED) microscopy is a super-resolution microscopy based on laser focusing microscopy, and its imaging speed is relatively fast, which provides a new method for research in the fields of biomedicine and materials. In the STED microscopic imaging device, two laser beams are required for illumination. One laser beam forms an Airy disk at the focal point of the objective lens, which excites the fluorescent substance and emits fluorescence; the other beam forms a ring with zero central light intensity at the focal point of the obj...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00
Inventor 苑立波邓洪昌黄宏阜罗浩
Owner GUILIN UNIV OF ELECTRONIC TECH
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