All-fiber STED super-resolution microscopic illumination device

Abstract

The invention provides an all-fiber STED super-resolution microscopic illumination device. The device mainly consists of a laser 1, a delay unit 2 and a beam generator 3, wherein the beam generator 3mainly consists of a double-clad fiber 4 and a polarization controller 5; the beam generator 3 can convert short-wave laser light 6 and long-wave laser light 7 emitted by the laser 1 into Gauss-shapedexcitation light 8 and hollow annular loss light 9, respectively; and since the light spot centers of the generated light are completely coincided and the light is irradiated on a fluorescent sample10 in sequence after the delay of the delay unit 2, stimulated emission depletion (STED) is generated, fluorescent light 11 with extremely small light spot is stimulated, and the final resolution is not limited by the diffraction of light, thereby breaking the diffraction limit and realizing all-fiber STED super-resolution microscopic illumination. The device has the advantages of highly integrating light beam generators and enabling equipment to be more flexible and stable.

Description

(1) Technical field [0001] The invention relates to the fields of fiber optic technology and optical microscope technology, in particular to an all-fiber stimulated emission depletion (STED) super-resolution microscopic illumination imaging device. (2) Background technology [0002] The development of modern biology and materials science has put forward higher and higher resolution requirements for microstructure research. Stimulated emission depletion (STED) microscopy is a super-resolution microscopy based on laser focusing microscopy, and its imaging speed is relatively fast, which provides a new method for research in the fields of biomedicine and materials. In the STED microscopic imaging device, two laser beams are required for illumination. One laser beam forms an Airy disk at the focal point of the objective lens, which excites the fluorescent substance and emits fluorescence; the other beam forms a ring with zero central light intensity at the focal point of the obj...

AI Technical Summary

Problems solved by technology

For example, the Chinese patent CN211817464U proposes a super-resolution fluorescence microscopy device based on tangential polarization. Although a good microscopic illumination effect is obtained, the realization of this effect requires complex optical paths, expensive precision instruments, and the stability is not very high.

Chinese patent CN103617330A proposes a super-resolution STED microscopic imaging device excited by a broadband laser light source generated by a supercontinuum. Although the function of synchronous adjustment of the two lasers is improved to achieve good microscopic illumination, the overall optical path is complicated

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