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Method for measuring active ingredient content in Ginkgo biloba L. leaves

A technology of active ingredients and ginkgo biloba, which is applied in the field of determining the content of active ingredients in ginkgo leaves, can solve the problems of complicated operation steps and insufficient simplicity of the process, and achieve the effects of good reproducibility, comprehensive quality and high stability

Active Publication Date: 2019-05-17
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0006] For the determination method of the content of active ingredients in Ginkgo biloba and its preparations, different detection methods are used in the prior art to detect the three types of active ingredients of ginkgo terpene lactones, flavonol glycosides and biflavones respectively. The disadvantage of this method is that different methods need to be used. Different instruments and different sample processing conditions are tested separately, resulting in complicated operation steps and insufficient process simplicity
Therefore, the simultaneous determination of ginkgolides, flavonol glycosides and biflavonoids in Ginkgo biloba leaves can be carried out to measure the content of three types of active ingredients, to make up for the imperfect and scientific deficiencies in the existing quality control technology of medicinal materials, and to accurately and comprehensively control the quality of Ginkgo biloba medicinal materials. , is of great significance

Method used

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  • Method for measuring active ingredient content in Ginkgo biloba L. leaves
  • Method for measuring active ingredient content in Ginkgo biloba L. leaves
  • Method for measuring active ingredient content in Ginkgo biloba L. leaves

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Embodiment 1

[0051] Take 1 g of ginkgo biloba medicinal material, add 100 mL of ethanol solution with a volume fraction of 50%, carry out high-speed homogenization (speed: 15×1000 rpm / min, time 1 min), ultrasonic extraction once for 60 min, dilute to 100 mL, pass through a 0.22 μm filter membrane Filter to obtain Ginkgo biloba need testing solution;

[0052] Weigh rutin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, bilobalide, ginkgolide A, ginkgolide acetonitrile solution, ginkgolide C, 7-de Methyl Ginkgo Biflavanone, Ginkgo Biflavanone, Iso-Ginkgo Biflavanone, Jinpine Biflavanone and internal standard compound isoquercitrin, Ginkgolide J, and spike filavone standard 1mg, dissolved in 1mL chromatographic methanol to obtain Ginkgo biloba Standard solution;

[0053] Using the mass spectrometry MRM detection mode, using the Ginkgo biloba standard as a template compound, the mass spectrometry parameters were optimized online, and the targeted ion pairs of the Ginkgo biloba standar...

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Abstract

The invention belongs to the technical field of chemical analysis and detection and particularly relates to a method for measuring active ingredient content in Ginkgo biloba L. leaves. According to the method, rapid online optimization is carried out on mass spectrometry parameters comprising a fragmentation voltage and collision energy in an electrospray anion tandem mass spectrometry multiple reaction monitoring (MRM) mode through application of an ultra performance liquid chromatography-tandem quadrupole mass spectrometer, and high-strength target ion pairs are formed. Through utilization of the target ion pairs with the series of characteristics, rapid qualitative and quantitative analysis is carried out on three kinds of active ingredients of ginkgolides, flavonol glycosides and biflavones in a ginkgo biloba medicinal material. The detection method provided by the invention is simple in operation and has high sensitivity and precision, good reproducibility, high stability and a high recovery rate. The three kinds of active ingredients of ginkgolides, flavonol glycosides and biflavones contained in the Ginkgo biloba L. leaves can be measured at the same time. The defect that anexisting medicinal material quality control technology is not complete and scientific enough is made up. Quality of the Ginkgo biloba L. medicinal material can be accurately and comprehensively controlled.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for measuring the content of active ingredients in ginkgo leaves. Background technique [0002] Ginkgo biloba is the dried leaf of Ginkgo biloba L., a plant of the Ginkgo family. It has the functions of promoting blood circulation and removing blood stasis, dredging collaterals and relieving pain, astringing the lungs and relieving asthma, and is often used to treat cardiovascular and cerebrovascular diseases and Alzheimer's disease. The chemical composition of Ginkgo biloba is complex. More than 170 compounds have been discovered so far, mainly flavonoids, terpene lactones, alcohols, alkaloids, polyisoprene compounds, polysaccharides and other pharmacologically active compounds. Element. [0003] According to the 2015 edition of the "Chinese Pharmacopoeia", the quality control indicators of Ginkgo biloba medicinal materials are only two types of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 付玉杰王立涛杨清孟冬牛丽丽樊小红简悦
Owner BEIJING FORESTRY UNIVERSITY
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