Method for measuring active ingredient content in Ginkgo biloba L. leaves

Abstract

The invention belongs to the technical field of chemical analysis and detection and particularly relates to a method for measuring active ingredient content in Ginkgo biloba L. leaves. According to the method, rapid online optimization is carried out on mass spectrometry parameters comprising a fragmentation voltage and collision energy in an electrospray anion tandem mass spectrometry multiple reaction monitoring (MRM) mode through application of an ultra performance liquid chromatography-tandem quadrupole mass spectrometer, and high-strength target ion pairs are formed. Through utilization of the target ion pairs with the series of characteristics, rapid qualitative and quantitative analysis is carried out on three kinds of active ingredients of ginkgolides, flavonol glycosides and biflavones in a ginkgo biloba medicinal material. The detection method provided by the invention is simple in operation and has high sensitivity and precision, good reproducibility, high stability and a high recovery rate. The three kinds of active ingredients of ginkgolides, flavonol glycosides and biflavones contained in the Ginkgo biloba L. leaves can be measured at the same time. The defect that anexisting medicinal material quality control technology is not complete and scientific enough is made up. Quality of the Ginkgo biloba L. medicinal material can be accurately and comprehensively controlled.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for measuring the content of active ingredients in ginkgo leaves. Background technique [0002] Ginkgo biloba is the dried leaf of Ginkgo biloba L., a plant of the Ginkgo family. It has the functions of promoting blood circulation and removing blood stasis, dredging collaterals and relieving pain, astringing the lungs and relieving asthma, and is often used to treat cardiovascular and cerebrovascular diseases and Alzheimer's disease. The chemical composition of Ginkgo biloba is complex. More than 170 compounds have been discovered so far, mainly flavonoids, terpene lactones, alcohols, alkaloids, polyisoprene compounds, polysaccharides and other pharmacologically active compounds. Element. [0003] According to the 2015 edition of the "Chinese Pharmacopoeia", the quality control indicators of Ginkgo biloba medicinal materials are only two types of...

AI Technical Summary

Problems solved by technology

[0006] For the determination method of the content of active ingredients in Ginkgo biloba and its preparations, different detection methods are used in the prior art to detect the three types of active ingredients of ginkgo terpene lactones, flavonol glycosides and biflavones respectively. The disadvantage of this method is that different methods need to be used. Different instruments and different sample processing conditions are tested separately, resulting in complicated operation steps and insufficient process simplicity

Therefore, the simultaneous determination of ginkgolides, flavonol glycosides and biflavonoids in Ginkgo biloba leaves can be carried out to measure the content of three types of active ingredients, to make up for the imperfect and scientific deficiencies in the existing quality control technology of medicinal materials, and to accurately and comprehensively control the quality of Ginkgo biloba medicinal materials. , is of great significance

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