Biochar-mediated solid biofilm anaerobic digestion accelerator preparation device and method thereof
An anaerobic digestion and preparation device technology, which is applied to biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems such as long start-up time, and achieve improved adequacy, rapid enrichment, The effect of shortening the hydraulic retention period
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Embodiment 1
[0060] A biochar-mediated solid biofilm anaerobic digestion accelerator preparation device, comprising a drum 5, a biogas slurry chamber 13, a biofilm culture chamber 20, an outer chamber 14, a constant temperature heater 26 and a vacuum freeze dryer 25; The drum 5 is located in the biogas slurry chamber 13; the biogas slurry chamber 13 is separated from the biofilm culture chamber 20 by a partition 19, and forms a jacket 21 with the outer chamber 14; the partition 19 There is a biogas slurry gate 6, and the biogas slurry gate switch 7 is located at the corresponding position on the outer wall of the outer cavity 14; the heating tube 15 of the constant temperature heater 26 is connected to the upper right end surface of the left wall of the outer cavity 14, and the heat return tube 16 is connected to the The lower right end surface of the right wall of the outer chamber 14; the biofilm gate 18 is provided between the bottom surface of the biofilm culture chamber 20 and the bott...
Embodiment 2
[0074] A preparation method of a biochar-mediated solid biofilm anaerobic digestion accelerator, based on a biochar-mediated solid biofilm anaerobic digestion accelerator preparation device of embodiment 1, comprising the following steps:
[0075] S1 Preparation of anaerobic digestion solid biofilm:
[0076] S1.1 medium preparation:
[0077] Medium: NH 4 Cl 1 g / L, MgCl 2 1 g / L, NaCl 0.5 g / L, K 2 HPO4 0.4 g / L, KH 2 PO4 0.4 g / L, peptone 2 g / L, yeast extract powder 2 g / L, sodium acetate 10 g / L, trace elements 10 mL, vitamin 10 mL, L-cysteine 0.5 g / L, 2‰ edge Azure 2 mL, distilled water 1000 mL (pH 7.0); among them,
[0078] Trace elements (g / L): N (CH2COOH) 3 4.5 (aminoacetic acid), FeCl 2 4H 2 O 0.4, MnCl H 2 O0.1, CoCl 2 ·6H 2 O 0.12, AlK ( SO 4 ) 2 0.01, ZnCl 2 0.1, NaCl 1, CaCl 2 0.02, Na 2 MoO 4 0.01, H 3 BO 3 0.01, NiCl 2 ·6H 2 O 0.42;
[0079] Vitamins (g / L): Biotin 2.0, Thiamine 5.0, Pyridoxine Hydrochloride 10, D-Pantocalcic Acid 5.0, Alp...
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