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High-flux microarray detection chip, and preparation method and utilization method of high-flux

A microarray chip and high-throughput technology, which is applied in the field of biomedical detection, can solve the problems of increasing and affecting the detection accuracy, and achieve more sensitive detection, fast detection, and increased detection accuracy.

Active Publication Date: 2019-06-07
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing microarray chips are mostly planar chips, and planar microarrays are limited to only one type of surface, so only one type of probe molecule can be placed, while microarray chips can simultaneously and rapidly detect multiple different Increasing demand for classes of biomolecular detection targets
In addition, the traditional planar microarray chip still has diffusion phenomenon, and the false positive probability of non-specific adsorption occurs, which affects the detection accuracy to a certain extent.

Method used

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Embodiment 1

[0048] Such as figure 1 As shown, the present invention provides a high-throughput microarray chip, which sequentially includes a porous film base layer 1 and an organic polymer array layer 2 from bottom to top. Micropores are arranged in the organic polymer array layer, and micropores are accommodated in the micropores. The detection microparticle 3 adapted to the shape of the microwell, the detection microparticle is coated with an antibody.

[0049] A microarray chip is a single area of ​​a chip, and multiple single areas can be integrated into an ultra-high-throughput detection chip. The size of a single area of ​​a microarray chip can be (5-100)mm*(5-100)mm, and its size can be adjusted appropriately according to the throughput of the microarray. Chip size fits.

[0050] The micropores arranged in the organic polymer array layer can be set as one or more of circles, stars, triangles, rounded rectangles, ovals, rectangles, and polygons, and the sizes of the micropores ca...

Embodiment 2

[0057] figure 2 A flow chart of a method for preparing a high-throughput microarray detection chip is provided, such as figure 2 shown, including the following steps:

[0058] Step 1: Place the porous film base 6 on the air-permeable thermoplastic 7, place a carrier under the air-permeable thermoplastic, and place the PDMS mold 4 with protrusions on the bottom surface according to the micropore array pattern on the porous film base;

[0059] Step 2: Open the sample injection hole on the PDMS mold, the position of the punched hole is outside the microhole array, and perform plasma cleaning on the PDMS mold after the hole is punched. Drop the liquid 5 to be cured (such as NOA81 photoresist) used to prepare the organic polymer array layer at the injection hole position, cover the injection hole, and keep the vacuum. At this time, the air in the PDMS mold will escape from the injection hole. The escaping air forms bubbles in the liquid to be solidified near the injection hole,...

Embodiment 3

[0067] image 3 A high-throughput microarray chip of the present invention is provided to detect the method for transfer and recovery of microparticles, such as image 3 shown, including the following steps:

[0068] Step 1: Pour the organic prepolymer solution 16 to be photocured on the microarray chip 13 equipped with detection microparticles, so that it spreads freely to cover the surface of the chip, and put a layer of the same composition as the organic prepolymer solution on it. cured substrate 15;

[0069] Step 2: Curing the target area by irradiating ultraviolet light 14, forming a covalent bond between the detection microparticles assembled on the microarray chip and the cured organic prepolymer 16, separating the microarray chip and transferring the detection microparticles, and retaining The relative position of the microparticles on the microarray chip.

[0070] By transferring and recovering the detection microparticles assembled on the microarray detection chi...

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Abstract

The invention provides a high-flux microarray chip, and a preparation method and a utilization method of the high-flux microarray chip. The high-flux microarray chip comprises a porous thin-film substrate layer and an organic polymer array layer sequentially from bottom to top, wherein micropores are distributed in the organic polymer array layer, and detection microparticles which are matched with the micropores in shape are contained in the micropores and coated with antibodies. The microarray chip disclosed by the invention is characterized in that the microarray chip combined with the detection microparticles is used to replace the traditional micro-sample application method, so that the bonding area of an object to be detected and probe molecules is increased and more rapid and more sensitive biomolecular detection can be realized; thus, the microarray chip can be used for the detection of a low-concentration target object; and the sensitivity and accuracy of the chip are improvedeffectively.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and in particular relates to a high-throughput microarray chip, a preparation method and an application method thereof. Background technique [0002] Parallel detection of multiple disease markers can reveal key information about a complex disease and provide an important basis for clinical diagnosis, which is difficult to obtain from the detection of a single target. For example, the analysis of different protein markers in serum can be used as an important basis for the diagnosis of diseases such as cancer, heart disease and cystic fibrosis. However, traditional ELISA and immunofluorescence detection methods require a large number of samples and reagents. The operation is complicated and there are many factors affecting the reaction, so it is difficult to realize simple and rapid detection of multiple target molecules at the same time. [0003] Microarray chips are characterized by high-den...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/53G01N33/541G01N33/58
Inventor 吕雪飞赵可心李永瑞邓玉林
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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