MOF (metal-organic framework)-SOD (superoxide dismutase) nano-assembly with anti-aging effect and preparation method of nano assembly
A metal-organic framework and dismutase nanotechnology, applied in the direction of oxidoreductase, drug combination, anti-toxic agent, etc., can solve the problems of poor gastrointestinal tract absorption capacity, poor active transmembrane ability, and limit the application of SOD, and achieve a good temperature The effects of tolerance, improvement of health status, and ability to resist external adverse environments
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Embodiment 1
[0020] Add 2 mL of dimethylimidazole aqueous solution (160 mM) into 2 mL of zinc acetate aqueous solution (40 mM) dissolved with 1 mg of SOD, and the solution temperature is 25°C. Then 200 μL of methanol was added to initiate the reaction, and the reaction was carried out at room temperature for 32 h. The system was centrifuged at 8000rpm / min for 5min, and the precipitate was collected. The precipitate was washed 3 times with distilled water, and then freeze-dried to obtain the final product SOD@ZIF-8 nano-assembly with a product mass of 10 mg. From figure 1 The scanning electron microscope of the synthesized product found that the SOD@ZIF-8 synthesized by the above method was approximately diamond-shaped nano-scale particles with a regular shape and a particle size between 200 and 400 nm.
[0021] The synthesis process of the empty MOF material me-ZIF-8 without SOD enzyme is similar to the above, the only difference is that no SOD is added during the synthesis process. That...
Embodiment 2
[0023] Add 4 mL of dimethylimidazole aqueous solution (160 mM) into 4 mL of zinc acetate aqueous solution (40 mM) dissolved with 2 mg of SOD, and the solution temperature is 25°C. Then, 400 μL of methanol was added to initiate the reaction, and the reaction was carried out at room temperature for 32 h. The system was centrifuged at 8000rpm / min for 5min, and the precipitate was collected. The precipitate was washed 3 times with distilled water, and then freeze-dried to obtain the final product SOD@ZIF-8 nano-assembly with a product mass of 20mg. From figure 2 The scanning electron microscope of the synthesized product found that the SOD@ZIF-8 synthesized by the above method is approximately diamond-shaped nano-scale particles with a regular shape, and its particle size is between 200 and 400nm. The SOD@ZIF-8 synthesized by the method of example 1 is consistent.
Embodiment 3
[0025] After fully drying the SOD@ZIF-8 and me-ZIF-8 synthesized in Example 1, the thermogravimetric experiment was carried out. The TAQ500 detection instrument was used to raise the temperature at a rate of 10°C / min in a nitrogen environment. The obtained thermogravimetric curve is as follows: image 3 shown. SOD@ZIF-8 has obvious weight loss at 100-200 °C, which is about 2% higher than that of me-ZIF-8. The higher weight loss represents the mass ratio of the enzyme. Therefore, according to the thermogravimetric curve, the SOD loading amount in SOD@ZIF-8 was calculated to be 2.0%, that is, 2 mg of SOD enzyme was contained in every 100 mg of SOD@ZIF-8.
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