Method for measuring contents of three kinds of components in aspalathus linearis (brum.f.) r.dahlgren, aspalathus linearis (brum.f.) r.dahlgren extractive, and aspalathus linearis (brum.f.) r.dahlgren tea

Abstract

The invention, which belongs to the technical field of food quality control, specifically provides a method for measuring contents of three kinds of components in the aspalathus linearis (brum.f.) r.dahlgren, the aspalathus linearis (brum.f.) r.dahlgren extractive, and the aspalathus linearis (brum.f.) r.dahlgren tea. The method comprises: step one, preparing a mixed reference solution; step two,preparing a test sample solution; step three, carrying out chromatographic condition and system adaptability testing; to be specific, carrying out measurement based on the high performance liquid chromatography under the conditions of using the octadecylsilane chemically bonded silica column as a column, acetonitrile as a mobile phase A, an acetic acid solution with the volume concentration of 0.8to 1. 5% or formic acid with the volume concentration of 0.1 to 0.5% as mobile phase B; carrying out chromatographic analysis according to a gradient elution procedure under conditions of controllingthe flow rate of the mobile phase of 0.8 to 1.2 ml / min and the detection wavelengths of 275 to 295nm and 340 to 360nm; and step four, performing a measuring method; to be specific, abstracting the mixed reference solution and the test sample solution respectively and injecting the solutions into a liquid chromatograph and measuring and calculating contents of isoorientin, orientin, and aspalathine. The invention provides a method for measuring contents of three kinds of components in the aspalathus linearis (brum.f.) r.dahlgren, the aspalathus linearis (brum.f.) r.dahlgren extractive, and theaspalathus linearis (brum.f.) r.dahlgren tea rapidly, accurately, and conveniently.

Description

technical field [0001] The invention belongs to the technical field of food quality control, and in particular relates to a method for measuring the contents of three components in gorse laceae, gorse laceae extract and gorse laceae tea. Background technique [0002] Gorse linearis is a South African leguminous plant (Latin scientific name: Aspalathus Linearis (Brum.f.) R.Dahlgren), and it is a new food raw material announced by the National Health and Family Planning Commission. The extract of gorse is the water extract of gorse, and the tea of ​​gorse is a common drink containing the extract of gorse. Line-leaf gorse is rich in antioxidant flavonoids, which have a significant effect on eliminating free radicals, regulating human body functions, preventing and controlling various diseases. Line-leaf gorse does not contain caffeine and oxalic acid, and has a calming center Nervous system effect, suitable for people who suffer from allergies, headaches, sleep disorders, inso...

AI Technical Summary

Problems solved by technology

Because orientin and isoorientin are isomers, it is difficult to effectively separate them, and the content of aspartame in the fermented gorse is relatively low, so it is not easy to extract. In addition, the components of gorse Numerous, complex, high impurity content, currently there is no quality control standard for wired gorse

The HPLC assay method of aspartin in rooibos tea is disclosed in the document "Determination of Aspartin in Rooibos". quality, the measurement result is single, and it is impossible to comprehensively detect the line-leaf gorse, and the method has the problems of complicated extraction operation, low resolution, poor peak shape, and long running time (see Cha Shenghua et al. Content determination of Batin, Natural Product Research and Development, 2009,21:414-415)

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