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Antibacterial gold nanoparticles modified by tetrazole or derivatives thereof, and preparation method and application thereof

A gold nanoparticle and nanoparticle technology, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve the complex synthesis process of ligands, lack of generalization, drug resistance and other problems , to achieve the effect of overcoming the synthetic route, good antibacterial effect and improving antibacterial activity

Active Publication Date: 2019-07-12
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, many of the above studies mainly have the following problems in clinical application: 1. The ligand is a commercial antibacterial drug, which has the same chemical structure as the drug that bacteria are resistant to, and even if it is put into clinical use, it will quickly produce drug resistance 2. The ligand requires a complex synthesis process, which is not generalizable and has a low screening rate; 3. The antibacterial spectrum is narrow, and it only has inhibitory effect on a single Gram-positive or Gram-negative bacteria, lacking a broad spectrum 4. Many developed antibacterial agents need external forces such as infrared, and clinical application will cause inconvenience to patients

Method used

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  • Antibacterial gold nanoparticles modified by tetrazole or derivatives thereof, and preparation method and application thereof
  • Antibacterial gold nanoparticles modified by tetrazole or derivatives thereof, and preparation method and application thereof
  • Antibacterial gold nanoparticles modified by tetrazole or derivatives thereof, and preparation method and application thereof

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Embodiment 1

[0057] Example 1: Preparation of Gold Nanoparticles Au_DAPT Modified by Substituted Tetrazolium

[0058] Preparation of A reaction mixture:

[0059] Add 100 μL of 1 mmol / mL chloroauric acid aqueous solution and 100 μL of 1 mmol / mL mercaptotetrazolium (PTT, HPTT, MMT) aqueous solution to a 50 mL round bottom flask, dilute the solution to 15 mL with ice water, and then add 8 mg Temperature 80, stirring in ice bath for 10 minutes.

[0060] Reduction and purification of B gold nanoparticles:

[0061] The molar ratio of chloroauric acid to sodium borohydride is 1:3, add sodium borohydride ice solution to the reaction mixture, stir rapidly for 15 minutes, and then stir slowly for 1 hour to stabilize the ligand. Use a dialysis bag with a molecular weight cut-off of 14,000 MW for 48 hours and change the water every 4 hours to remove unreacted raw materials and impurities. The obtained gold nanoparticle solution is wine red and can be stored stably in a refrigerator at 4°C for at ...

Embodiment 2

[0062] Embodiment 2: Gold nanoparticle electron microscope sample preparation and observation

[0063] Hold a piece of copper mesh with tweezers to keep it horizontal, use a pipette gun to draw 10 μL of gold nanoparticle solution and drop it vertically onto the surface of the copper mesh, keep it for 2 minutes, then use filter paper to absorb the solution from the edge of the copper mesh, place it in a desiccator overnight, and use Observation by transmission electron microscope. The result is as figure 2 shown. The particle size of MMT-modified gold nanoparticles is the largest, which is 5.49±0.7nm; followed by Au_PTT gold nanoparticles, with a particle size of 2.63±0.62nm; the smallest particle size is Au_HPTT gold nanoparticles, with a particle size of only 1.78±0.28nm. The size of gold nanoparticles is closely related to the surface-modified molecules. The methyl group of No. 1 molecule has a linear structure, and its role in stabilizing gold nanoparticles is not as l...

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Abstract

The invention provides antibacterial gold nanoparticles modified by tetrazole or derivatives thereof, and a preparation method and an application thereof. A substituted tetrazole part without antibacterial activity is connected with non-substituted gold nanoparticles without antibacterial activity, and the antibacterial gold nanoparticles are firstly applied in the antibacterial field; the obtained gold nanoparticles modified with substituted tetrazole have unexpected antibacterial effect. The synthesized gold nanoparticles have good antibacterial effect on both gram-negative bacteria and gram-positive bacteria, have broad antibacterial spectrum and can resist clinically isolated multi-drug resistant bacteria. Compared with the prior art, the gold nanoparticles are connected with mercapto-tetrazole compounds, the synthetic method adopts one-step synthesis in an aqueous phase, and the raw materials used in the synthesis are green and environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field, and specifically relates to an antibacterial gold nanoparticle modified by tetrazolium or derivatives thereof, a preparation method and application thereof. Background technique [0002] The widespread use and irrational abuse of antibacterial drugs has made the problem of bacterial drug resistance a widespread problem in my country and even the world, which has brought huge losses to people's health and social economy. It is one of the most difficult and urgent problems in clinical practice. . Facing the increasingly serious bacterial resistance to human health, in addition to advocating and promoting the rational use of antibacterial drugs, it is very important to accelerate the development of effective and safe antibacterial drugs. The development of antibacterial drugs is currently mainly based on two aspects, one is the discovery of natural antibacterial ingredients, and the other is synthetic antibact...

Claims

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Application Information

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IPC IPC(8): A61K31/41A61K31/24A61P31/04A01N59/16A01N43/713A01P1/00
CPCA61K31/41A61K31/24A01N59/16A01N43/713A61K2300/00Y02A50/30
Inventor 蒋兴宇田月
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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