Liquid culture method of a kind of fungal strain and mycelia

The technology of mycelium and strain is applied in the field of fungal strain and mycelium liquid culture, which can solve the problems of unsatisfactory growth rate of fungi, reduced activity of fungal fruiting body, incomplete sterilization and disinfection, etc., and achieves good economic benefits and Social effects, shorten the production cycle, enhance the effect of immunity

Active Publication Date: 2021-04-13
NORTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the expanded cultivation of fungi, the traditional solution is solid culture method, which is relatively mature and standardized, but the growth rate of fungi is not ideal.
The current conventional liquid culture method mostly directly inoculates the fungal sclerotia in the liquid medium. Although 1% sodium hypochlorite solution will be used for disinfection and sterilization before inoculation, if the concentration of sodium hypochlorite is too low, the sterilization will not be complete. , in the case of easy pollution, if the concentration is too high, the activity of the fungal fruiting body will be reduced or even lose its biological activity.
Therefore, although the growth rate of fungi in the conventional liquid culture method is better than that in the solid culture method, the cultivation of some strains is not ideal in actual production due to insufficient stability.

Method used

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  • Liquid culture method of a kind of fungal strain and mycelia
  • Liquid culture method of a kind of fungal strain and mycelia
  • Liquid culture method of a kind of fungal strain and mycelia

Examples

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preparation example Construction

[0023] 2. Preparation of strains—activated strains

[0024] The collected fungal sclerotia was washed several times with 1% (v / v) NaClO solution, soaked for 2-3min each time, after pouring off the waste liquid, washed off its surface residual liquid with sterile water, and then inoculated on Place the prepared slant culture medium at 24-27°C for 3-4 days. After the cultivation is complete, select the strains with better growth and store them at 4°C for later use.

[0025] 3. Preparation of mother liquor

[0026] Prepare PDA liquid culture medium (agar may not be added), subpackage, and after sterilization, use a sterile inoculation needle to transfer the preserved mycelium species into the PDA liquid culture medium in a sterile operating table, and put it into a shaker at 24-27°C. Bed shaking culture 3-4d, namely the mycelium mother liquor.

[0027] 4. Liquid culture of mycelium

[0028] (1) Optimal liquid medium formula

[0029] Corn grits 35g, peptone 10g, glucose 15g, p...

Embodiment 1

[0062] After feeding 40 clean-grade ICR mice (male, body weight 20g±2g) for 2 days, they were divided into 4 groups A, B, C, and D, and the recommended dosage for human oral administration was set at 300, 600, and 1200 mg / kg bw ( Respectively equivalent to 5, 10, 20 times of human recommended dosage) 3 dosage groups and a negative control group. Weigh 1.5, 3.0, and 6.0 g of mycelium fine powder, respectively, add pure water to 100 mL, respectively make 15, 30, and 60 g / L concentration solutions, and give them to gavage, the volume of gavage is 0.2 mL / 10 g bw, negative The control group was given an equal volume of pure water, orally administered once a day, and the indicators were measured after continuous intragastric administration for 30 days, and the mice in group D were used as blank control. The test results show that in the (mouse) weight-bearing swimming test: the average weight-bearing swimming time of the mice in group A was 23 minutes, the average weight-bearing swi...

Embodiment 2

[0064] After feeding 40 clean-grade ICR mice (male, body weight 20g±2g) for 2 days, orally administered three doses of 300, 600, and 1200 mg / kg bw of mycelium powder solution for 30 days, the test was carried out . The experimental results showed that in the mouse delayed-type allergic reaction (toe swelling) experiment, the 600 and 300mg / kg·bw two dose groups compared with the control group mice had significant differences in the degree of swelling of the paws. In the spleen lymphocyte transformation experiment (MTT method), the difference in optical density between the 600mg / kg·bw dose group and the control group was significantly different. In the mouse peritoneal macrophage phagocytosis experiment of chicken red blood cells, the 1200mg / kg·bw dose group had significant differences in the phagocytosis percentage and phagocytosis index of chicken red blood cell phagocytosis by peritoneal macrophages compared with the control mice. In the mouse NK cell activity experiment, th...

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Abstract

The invention provides a liquid culture method for a novel fungal strain and its mycelium. The fungi are Fibularhizoctonia sp. fungi of Basidiomycota, Agaricomycetes, Atheliales, and Atheliaceae, and the preservation number is CCTCC M 2018446. The mycelium of the strain is rich in polysaccharides and triterpenoids, and can be developed and applied as a new type of edible fungus. The mycelium obtained by the liquid culture method of the invention has good growth and high mycelium yield, and the artificial liquid culture of the strain is successfully realized.

Description

technical field [0001] The invention relates to a novel fungal strain and a liquid culture method for mycelia. Background technique [0002] There are many kinds of fungi on the earth, rich in resources, and many of them are also the daily food that humans love, including fungi that can be used as daily food or as medicine. However, no more than 10% of the known fungi have been used by people. In addition to the well-known edible fungi such as shiitake mushrooms, fungus, and oyster mushrooms, as well as medicinal fungi such as Ganoderma lucidum and Cordyceps, there are still a large number of large-scale fungal resources that need to be developed and utilized urgently. . Although there are a large number of wild fungi, only less than 3.4% of them can be artificially cultivated, and most of them are in a state of natural growth. The domestication research of wild fungi still has great potential, and there are many species with good texture and edible or medicinal value. . ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00A61K36/07A61P37/04A61P39/00A61P3/06A61P3/10A23L31/00A23L33/00C12R1/645
CPCA01H15/00A23V2002/00A61K36/07A23L31/00A23L33/00A61P3/06A61P3/10A61P37/04A61P39/00C12N1/14C12N1/145C12R2001/645A23V2200/30A23V2200/324A23V2200/31A23V2200/3262A23V2200/328
Inventor 邢连喜张宏贵赵剑
Owner NORTHWEST UNIV
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