Method for measuring trace salbutamol in environmental sample

Abstract

The invention discloses a method for measuring trace salbutamol in an environmental sample. The method comprises the following steps: preparing materials and reagents, preparing a buffer solution, selecting instruments, preparing a Eu3+ labeled goat anti-rabbit antibody, covering a microwell plate with a coating antigen in CBS, and performing incubation, performing culture in TBS by using OVA to block the microwell plate, adding a polyclonal antibody diluted by a methanol standard solution and PBS, adding a diluted Eu3+ labeled antibody in the PBS for incubation, adding an enhancement solution, performing mechanical oscillation, and using a microtitrimeter to measure the fluorescence intensity at an excitation wavelength of 340 nm, an emission wavelength of 615 nm, a delay time of 100 mu sand a window time of 400 mu s. The method disclosed by the invention has the advantages of high sensitivity and detection capability, good reproducibility, high recovery rate, and capability of meeting the requirements of residue analysis.

Description

technical field [0001] The invention belongs to the technical field of chemical determination, and relates to a method for determining trace salbutamol in environmental samples by a high-sensitivity time-resolved fluorescence immunoassay. Background technique [0002] Veterinary medicine plays an important role in protecting the livestock breeding industry. However, the widespread use of veterinary drugs and feed additives has become an important factor in environmental pollution and harm to human health (Bártíková et al., 2016; Emanuela et al., 2019). Salbutamol (salbutamol, SAL), an adrenergic receptor agonist, is often illegally abused as a "lean drug" in livestock production. Studies have shown that SAL has certain toxicity to human heart, liver and kidney (Susan, 1994; Khirani et al., 2016). Although SAL has been strictly banned as a growth-promoting feed additive in many countries, it is still widely used as a clinical drug (Zhang et al., 2017; Marchant-Forde et al.,...

AI Technical Summary

Problems solved by technology

However, these immunoassays mainly focus on SAL detection in animal products, feed, and urine, and are rarely applied to environmental samples (Xu et al., 2015; Li et al., 2017)