Bifidobacterium pseudocatenulatum CCFM1045, composition, fermented food, applications, microbial inoculum and preparation method thereof

A bifidobacteria and fermented food technology, applied in the field of microorganisms, can solve problems such as stimulation of the gastrointestinal tract, liver function damage, and impact on patients, and achieve the effects of improving abnormality, improving elevation, and reducing Parkinson's

Active Publication Date: 2019-08-06
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These drugs have a good curative effect on each single risk factor of metabolic syndrome. However, long-term use of drugs has certain side effects. For example, long-term use of metformin can cause irritat

Method used

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  • Bifidobacterium pseudocatenulatum CCFM1045, composition, fermented food, applications, microbial inoculum and preparation method thereof
  • Bifidobacterium pseudocatenulatum CCFM1045, composition, fermented food, applications, microbial inoculum and preparation method thereof
  • Bifidobacterium pseudocatenulatum CCFM1045, composition, fermented food, applications, microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: Pseudo-small chain Bifidobacterium CCFM1045 has good tolerance to simulated gastrointestinal fluid

[0085] Inoculate the cryopreserved Bifidobacterium CCFM1045 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and then subculture in mMRS medium for 2 After ~3 times, take 1mL of the culture solution of Bifidobacterium pseudospermia CCFM1045, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and anaerobically For culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0086]The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the time of 0h in the culture fluid, expressed in %. T...

Embodiment 2

[0091] Example 2: Pseudomonas Bifidobacterium CCFM1045 has no toxic side effects on C57BL / 6JBL / 6J mice

[0092] Resuspend the pseudosmall chain Bifidobacterium CCFM1045 bacteria in 3% sucrose solution to make a concentration of 3.0 × 10 8 CFU / mL bacterial suspension. Take 6 healthy male C57BL / 6JBL / 6J mice with a body weight of about 20g. After acclimating to the environment for a week, they were given the bacterial suspension of this concentration by gavage once a day, observed for a week, and recorded the death and body weight.

[0093] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The pseudo small chain bifidobacterium CCFM1045 of CFU / mL did not cause significant impact on mice, and the body weight had no significant change, and no death phenomenon occurred. The appearance of the mice had no obvious pathological symptoms.

[0094] Table 3 Changes in body weight and death of mice

[0095]

[0096] Note...

Embodiment 3

[0097] Embodiment 3: Pseudosmall chain Bifidobacterium CCFM1045 has recovery effect to the intestinal flora disorder caused by high-fat feed

[0098] Take 36 healthy male C57BL / 6JBL / 6J mice weighing 18-20g, adapt to the environment for 1 week, and randomly divide them into 6 groups: blank control group (NC), high fat model control group (HFD), simvastatin control group (SC), rosiglitazone control group (RH), Bifidobacterium pseudo small chain CCFM1045 intervention group (CCFM1045), Bifidobacterium animalis BB12 control group (BB12). The dose of liquid is 3.0 x 10 8 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0099] Table 4 Grouping of experimental animals

[0100]

[0101]

[0102] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the metagenome in the feces was extracted, and the structure of the intestinal flora was analyzed using a next-gen...

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Abstract

The invention discloses bifidobacterium pseudocatenulatum CCFM1045, a composition, a fermented food, applications, a microbial inoculum and a preparation method thereof. As the bifidobacterium pseudocatenulatum CCFM1045 can tolerate gastrointestinal environments in human bodies, the abnormity of metabolic syndrome mouse serum liver function indexes ALT and AST caused by high-fat diet can be significantly improved; the abnormal oral glucose tolerance of metabolic syndrome mice can be significantly improved as well; area under the curve of glucose tolerance can be reduced; serum insulin levels can be decreased; the rising of triglycerides and total cholesterol content in serum of the metabolic syndrome mice caused by the high-fat diet can be significantly improved; and the rising of Proteusgenus in intestinal tracts caused by the high-fat diet can also be significantly improved. The bifidobacterium pseudocatenulatum CCFM1045 can be used for preparing the pharmaceutical composition and fermented food for alleviating metabolic syndromes and decreasing the occurrence of Parkinson, intestinal infection, urinary tract and kidney infection and other diseases, so that the bifidobacterium pseudocatenulatum CCFM1045 can have very wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Bifidobacterium pseudosmall chain CCFM1045, its composition, fermented food, use, bacterial agent and preparation method thereof. Background technique [0002] In recent years, with the continuous development of my country's social and economic level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased day by day, and the restrictions of many working conditions have made it easy for people to develop sedentary behavior Habit. These external environmental factors combined with their own genetic factors have jointly led to the continuous increase in the incidence of metabolic syndrome in the Chinese population. According to reports, between 2002 and 2010, the proportion of people over the age of 20 in China suffering from metabolic syndrome rose from 5.4% to 21.3%, an increase ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/123A23C13/16A23C19/032A23C11/10A23L11/00A23L33/00C12R1/01A23L11/45A23L11/50A23L11/60
CPCC12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/1234A23C13/16A23C19/0323A23C11/106A23L33/00A61K2035/115A23V2002/00A23L11/50C12R2001/01C12N1/205A23V2400/535A23V2200/3204A23V2200/3262A23V2200/328A23V2200/32A23V2200/322A23V2200/30Y02A50/30
Inventor 王刚陈卫刘煜飞赵建新张灏
Owner JIANGNAN UNIV
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