Artificial cornea and preparation method and application thereof

Abstract

The invention provides an artificial cornea and a preparation method and application thereof. The preparation method of the artificial cornea comprises the following steps that endothelial cells of acornea are subjected to penetrating inoculation onto the stroma side of a sterile antigen-extracted animal cornea endodermis, and after culture, the endodermis which is 15-27 micrometers thick is formed; stroma cells are subjected to penetrating inoculation onto a sterile antigen-extracted animal cornea endodermis and cultured to obtain an endodermis which is 400-550 micrometers thick; corneal epithelial cells and a sterile antigen-extracted animal cornea epithelial layer are cultured into an epithelial layer which is 50-68 micrometers thick; the endodermis, the stroma layer and the epitheliallayer are superposed in sequence, and after combined culture, the artificial cornea is obtained. The artificial cornea solves the problems about histocompatibility, transparency, mechanical strengthand diopter of corneal transplantation, and the artificial cornea can be directly used for corneal transplantation. The preparation method can be produced in batches, and the requirements of a lot ofcorneal blind patients can be met.

Description

technical field [0001] The invention relates to the field of tissue engineering, more specifically, to a synthetic cornea and its preparation method and application. Background technique [0002] The cornea is the window through which people know the world. Due to corneal blindness caused by various reasons, about 10 million people around the world are blinded by corneal diseases every year. The effective way to regain sight is corneal transplantation. However, donor corneal resources are scarce. Unable to meet the needs of patients. With the development of science and technology, the artificial cornea prepared by tissue engineering is expected to become a new cornea substitute for the treatment of diseases. So far, there are mainly several types of corneas engineered by group engineering: the first is organic synthetic corneas, such as polyglycolic acid, chitosan, chondroitin sulfate, etc., and organic synthetic corneas formed by cells. The disadvantage is that the mechan...

AI Technical Summary

Problems solved by technology

So far, there are mainly several types of corneas engineered by group engineering: the first is organic synthetic corneas, such as polyglycolic acid, chitosan, chondroitin sulfate, etc., and organic synthetic corneas formed by cells. The disadvantage is that the mechanical strength is not enough, and there is no corneal curvature, so it is difficult to be used for transplantation; the second is a temporary substitute, such as animal (pig, rabbit, etc.) deantigenated corneal stent, amniotic membrane, silk fibroin and other natural scaffolds plus cells The advantage of the formed cornea is that it can meet the requirements of mechanical strength, and the disadvantage is that the distribution of corneal cells in the scaffold is uneven, but it is effective for patients with stroma blindness (Liu Zuo, 2012); the third type is full-thickness cornea (Fan Xianqun, 2007) , such as animal cornea, human cornea, multilayer cornea, etc., the advantage is that it has the function of the cornea, and the disadvantage is that the source is limited and the rejection reaction