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Osteochondral scaffold containing calcified layer for slow release of dual growth factors and preparation method thereof

A growth factor and calcified layer technology, applied in the field of biomaterials and biomedical engineering, can solve the problems of growth factor inactivation and disorder

Active Publication Date: 2022-05-17
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of rapid inactivation of growth factors and disordered microenvironment of cartilage and bone existing in existing osteochondral scaffold materials, and prepares a slow-release double growth factor-containing osteochondral scaffold with calcified layers, so as to maintain growth factor activity and cartilage, bone, Stabilize the bone microenvironment and promote the regeneration of osteochondral

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Dissolve 50mg PLGA in 1mL dichloromethane as the oil phase (O), add 0.1mL containing 5μg of BMP-2 or TGF-β 3 Aqueous solution of growth factors (inner water phase W1), prepare colostrum (W1 / O) with homogeneous emulsifier, then add colostrum into 30mL aqueous solution (W2) containing 0.5% PVA and continue to prepare with homogeneous emulsifier Double milk (W1 / O / W2). The double emulsion was magnetically stirred at 600 rpm for 3 hours to solidify the microspheres. The solidified microspheres were centrifuged and washed 3 times with distilled water, frozen in a -80°C freezer, and PLGA microspheres loaded with growth factors were obtained after freeze-drying for 48 hours.

[0018] 2) Weigh silk fibroin and nano-hydroxyapatite solids at a mass ratio of 1:1 and dissolve them in hexafluoroisopropanol to prepare a mixed solution with a concentration of 8%, inject 1 mL of the mixed solution into 2 g of sodium chloride crystals Put the particles in a cylindrical mold, place th...

Embodiment 2

[0022] 1) Dissolve 100mg PLGA in 1mL dichloromethane as the oil phase (O), add 0.1mL containing 5μg of BMP-2 or TGF-β 3 Aqueous solution of growth factor (inner water phase W1), prepare colostrum (W1 / O) with homogeneous emulsifier, then add colostrum into 40mL aqueous solution (W2) containing 1% PVA and continue to prepare with homogeneous emulsifier Double milk (W1 / O / W2). The double emulsion was magnetically stirred at 600 rpm for 4 hours to solidify the microspheres. The solidified microspheres were centrifuged and washed 3 times with distilled water, frozen in a -80°C freezer, and PLGA microspheres loaded with growth factors were obtained after freeze-drying for 48 hours.

[0023] 2) Weigh silk fibroin and nano-hydroxyapatite solids in a mass ratio of 1:1 and dissolve them in hexafluoroisopropanol to prepare a mixed solution with a concentration of 8%, inject 1mL of the mixed solution containing 2g of sodium chloride crystals Put the particles in a cylindrical mold, place ...

Embodiment 3

[0027] 1) Dissolve 200mg PLGA in 1mL dichloromethane as the oil phase (O), add 0.1mL containing 5μg of BMP-2 or TGF-β 3 Aqueous solution of growth factor (inner water phase W1), prepare colostrum (W1 / O) with homogeneous emulsifier, then add colostrum to 50mL aqueous solution (W2) containing 2% PVA and continue to prepare with homogeneous emulsifier Double milk (W1 / O / W2). The double emulsion was magnetically stirred at 600 rpm for 4 hours to solidify the microspheres. The solidified microspheres were centrifuged and washed 3 times with distilled water, frozen in a -80°C freezer, and PLGA microspheres loaded with growth factors were obtained after freeze-drying for 48 hours.

[0028] 2) Weigh silk fibroin and nano-hydroxyapatite solids at a mass ratio of 1:1 and dissolve them in hexafluoroisopropanol to prepare a mixed solution with a concentration of 8%, inject 1 mL of the mixed solution into 2 g of sodium chloride crystals Put the particles in a cylindrical mold, place them a...

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Abstract

This paper discloses a slow-release double growth factor-containing osteochondral scaffold with calcified layer and its preparation method. In terms of materials, a calcified layer-containing osteochondral scaffold with the function of slow-release dual growth factors is formed. The constructed osteochondral scaffold has a structure similar to that of natural cartilage, including an upper layer with an oriented structure, a dense calcified middle layer, and a mesh-like porous lower layer. Polyester material microspheres containing slow-release bone morphogenetic protein growth factors maintain the stability of the cartilage and bone microenvironment through material structure design, and the differential slow release of factors promotes the functionalization of different regions of the scaffold, so that the constructed osteochondral scaffold has good Promote the effect of osteochondral repair and regeneration.

Description

technical field [0001] The invention relates to a calcified layer-containing osteochondral scaffold for slow-release double growth factors and a preparation method thereof, specifically, loading growth factor-loaded polyester material microspheres on a silk fibroin composite material to form a calcified layer-containing osteochondral scaffold and its preparation method. Preparation. It belongs to the field of biomaterials and biomedical engineering. Background technique [0002] Osteochondral joint injury caused by trauma, tumor resection, osteoarthritis, etc. is a relatively common disease in orthopedics. The current treatment methods for this combined osteochondral injury include autologous or allogeneic osteochondral transplantation, but there are problems of donor site injury or immune rejection, and the overall treatment effect is not good, and breakthrough progress has not been made until now. Tissue engineering technology provides a new idea for the treatment of ost...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/40A61L27/36A61L27/12A61L27/18A61L27/22A61L27/54A61L27/56
CPCA61L27/18A61L27/227A61L27/3633A61L27/40A61L27/12A61L27/54A61L27/56A61L2300/414A61L2300/252A61L2300/604A61L2430/02A61L2430/06C08L67/04C08L89/00
Inventor 王淑芳杨强董云生赵艳红马信龙李云洁张琳
Owner NANKAI UNIV
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