Detection method for content of chlorogenic acid in stauntonia chinensis DC
A detection method, the technology of chlorogenic acid, which is applied in the medical and biological fields, can solve the problems of inaccurate measurement results and low concentration, and achieve the effects of avoiding regeneration treatment operations, accurate detection results, and simple detection methods
Inactive Publication Date: 2019-08-16
贵州天楼生物发展有限公司
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The results show that: chlorogenic acid has a good linear relationship (r=1.0000) in the concentration range of 10.21~102.1 μg / ml, and the recovery rate is 98.90% (RSD=0.8%), but when the concentration of chlorogenic acid is below 10 μg / ml The assay effect of using this method is not accurate, and the wild papaya product of prior art such as wild papaya slice, wild papaya powder, its concentration is all lower and basically is lower than below 10 μ g / ml, therefore needs to re-establish a method for A method for the determination of chlorogenic acid content in wild papaya products at a concentration lower than 10 μg / ml without gradient elution
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[0018] The following is further described in detail through specific implementation methods:
[0019] 1 Materials and methods
[0020] 1.1 Materials and reagents
[0021] Chlorogenic acid standard was purchased from Aladdin Company, potassium dihydrogen phosphate (KH 2 PO 4 ), phosphoric acid were purchased from Guangzhou Guanghua Technology Co., Ltd.; methanol and acetonitrile were purchased from Aladdin Company; the water used was ultrapure water, which was made by our laboratory.
[0022] 1.2 Instruments and equipment
[0023] Waters H-Class ultra-high performance liquid chromatography (equipped with Waters PDA detector, empower chromatography management system); METTLER TOLEDO ten-thousandth balance; METTLER TOLEDO pH meter; eppendorf pipette gun; ELGA Chorus ultrapure water treatment system; Ningbo Xinzhi ultrasonic cleaning instrument.
[0024] 1.3 Method
[0025] 1.3.1 Chromatographic conditions
[0026] Thermo Fisher Accucore aQ (150x3mm, 2.6μm) chromatographic...
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The invention discloses a detection method for the content of chlorogenic acid in stauntonia chinensis DC. The detection method comprises the following steps that: 1) smashing a stauntonia chinensis DC product into powder, adding methyl alcohol, carrying out ultrasonic extraction for 30min, and filtering to obtain a sample to be detected; 2) weighing a chlorogenic acid standard product, then configuring 1mg / ml of chlorogenic acid reference substance stock solution by the methyl alcohol; 3) measuring by high performance liquid chromatography for measurement, wherein a stationary phase is an Accucore aQ chromatographic column, a moving phase A is phosphate buffered solution, a moving phase B is acetonitrile, the volume ratio of A to B is 85:15, a flow rate is 0.6mL / min, detection wavelengthis 325nm, a column temperature is 30DEG CC, and a sample feeding amount is 2[Mu]L; and 4) taking the peak area of the reference substance stock solution is a vertical coordinate, taking the concentration of the reference substance stock solution as an abscissa, drawing the standard curve of the chlorogenic acid, and calculating the content of the chlorogenic acid in the stauntonia chinensis DC ina sample to be detected. By use of the above method, the stauntonia chinensis DC product which contains the chlorogenic acid under low concentration can be effectively detected, detection time is short, and accuracy is high.
Description
technical field [0001] The present invention and the field of biotechnology specifically relate to a method for detecting the content of chlorogenic acid in wild papaya, more specifically a method for detecting the content of chlorogenic acid in wild papaya products at low concentrations. Background technique [0002] Wild papaya is a tropical fruit belonging to Akebiaceae, a tropical evergreen small tree), also known as iron foot pear, papaya, etc., enjoys the reputation of "fruit of all benefits", and is a unique plant in China. The natural, pure green, organic edible fruit that matches the reality is the most suitable leisure and therapeutic fruit for modern people. Distributed in Guizhou, Chongqing and other places in mainland China, the wild papaya in Zunyi, Guizhou is the most famous, and Zunyi Zheng'an is also known as the "hometown of wild papaya". [0003] Wild papaya is better with "sour taste". Papaya organic acid has complex composition and rich types, such as s...
Claims
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Login to View More IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/047
Inventor 张剑保玉心陈荣祥张勇淳泽利肖欣庹利
Owner 贵州天楼生物发展有限公司