Method for maintaining continuous hydrogen production after chlorella cell death

Abstract

The invention relates to a method for maintaining continuous hydrogen production after chlorella cell death. The invention belongs to the technical field of biology and particularly relates to a method for maintaining continuous hydrogen production after chlorella cell death. The invention aims to introduce a power supply system into the living environment of chlorella, and to realize high-efficiency and continuous hydrogen production in an anaerobic environment by utilizing hydrogenase in the chlorella body. The method comprises the following steps: taking chlorella pyrenoidosa as a basic organism, and constructing an integrated artificial hydrogen production system by using a biological enzyme-hydrogenase in the chlorella body through an external conductive medium pyrrole and an electronsupply system. The method enables the biological enzyme to be efficiently and fully utilized, not only can maintain the activity of life body of chlorella cells, but also can continue to utilize active substances in the body of the chlorella cells after the life body is dead, so that the chlorella pyrenoidosa can continuously and efficiently produce hydrogen in an anaerobic environment for more than two months.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for maintaining continuous hydrogen production after chlorella cell death. Background technique [0002] With the increasing development of the global economy, people's demand for energy is also increasing, and the resulting environmental problems are becoming increasingly urgent. Fossil energy is becoming increasingly exhausted. It is particularly important to find a substitute for fossil fuels such as coal and oil. As a kind of renewable energy and its large-scale production, biofuel has aroused the attention and thinking of more and more scientific and technological workers. [0003] As a clean and efficient energy source, hydrogen energy is widely favored by people because of its high calorific value and high energy conversion rate. However, there are still many problems in the current method of hydrogen production. Hydrogen production by electrolysis of wat...

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Problems solved by technology

At present, the methods of using hydrogenase include extracting hydrogenase from cells and simulating intracellular electron transfer mechanism to achieve hydrogen production [Brown KA, Wilker M B, Boehm M, et al.Characterization of Photochemical Processes for H2Production by CdS Nanorod–[ FeFe]Hydrogenase Complexes[J].Journal of the American Chemical Society,2012,134(12):5627-5636.] Preparation of oxygen-resistant catalase analog hydrogen by genetic engineering [Sakai T, Mersch D, Reisner E.Photocatalytic HydrogenEvolution with a Hydrogenase in a Mediator-Free System under High Levels of Oxygen**[J].Angewandte Chemie,2013,52(47):12313-12316.], biomimetic siliconization induced self-assembly hydrogen production[Wei Xiong,Xiaohong Zhao,Genxing Zhu , Changyu Shao, Yaling Li, Weimin Ma, Xurong Xu, and Ruikang Tang, Angew.Chem., 2015, 127, 12129.] and other methods, but the extraction process of natural enzymes is cumbersome and difficult to store, and the cost is high. Hydrogenase mimics The preparation is complex and has low activity, and the method of biomimetic silicification can only achieve hydrogen production in a short period of time.

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