Method for maintaining continuous hydrogen production after chlorella cell death

A technology of cell death and chlorella, which is applied in the biological field, can solve the problems of complex preparation of hydrogenase simulants, troublesome extraction process of natural enzymes, and difficult storage, and achieve the effects of long hydrogen production time, simple preparation method, and reduced pollution

Active Publication Date: 2019-08-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, the methods of using hydrogenase include extracting hydrogenase from cells and simulating intracellular electron transfer mechanism to achieve hydrogen production [Brown KA, Wilker M B, Boehm M, et al.Characterization of Photochemical Processes for H2Production by CdS Nanorod–[ FeFe]Hydrogenase Complexes[J].Journal of the American Chemical Society,2012,134(12):5627-5636.] Preparation of oxygen-resistant catalase analog hydrogen by genetic engineering [Sakai T, Mersch D, Reisner E.Photocatalytic HydrogenEvolution with a Hydrogenase in a Mediator-Free System ...

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  • Method for maintaining continuous hydrogen production after chlorella cell death
  • Method for maintaining continuous hydrogen production after chlorella cell death
  • Method for maintaining continuous hydrogen production after chlorella cell death

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specific Embodiment approach 1

[0022] Specific implementation mode 1: In this implementation mode, a method for maintaining continuous hydrogen production after the death of Chlorella cells is specifically carried out according to the following steps:

[0023] 1. Cultivation of Chlorella pyrenoidosa cells: Select Chlorella pyrenoidosa as a biological template, and use BG-11 medium to cultivate in a light incubator with a temperature of 25-30°C under continuous light of 1200-4800LUX. Coating is performed when the number of chlorella cells reaches the logarithmic growth phase to obtain chlorella pyrenoidosa cells in the logarithmic growth phase;

[0024] 2. Use deionized water to clean the chlorella pyrenoidosa cells in the logarithmic phase of growth, collect the cells by centrifugation, and add the collected cells to the ferric nitrate nonahydrate aqueous solution to obtain the cell solution; Stir for 20-40min under the condition of ~260r / min. After the reaction is completed, wash with deionized water and c...

specific Embodiment approach 2

[0028] Embodiment 2: This embodiment differs from Embodiment 1 in that: the concentration of the ferric nitrate nonahydrate aqueous solution in step 2 is 1.2 mg / mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0029] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the OD value of the cell solution in Step 2 is 1. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for maintaining continuous hydrogen production after chlorella cell death. The invention belongs to the technical field of biology and particularly relates to a method for maintaining continuous hydrogen production after chlorella cell death. The invention aims to introduce a power supply system into the living environment of chlorella, and to realize high-efficiency and continuous hydrogen production in an anaerobic environment by utilizing hydrogenase in the chlorella body. The method comprises the following steps: taking chlorella pyrenoidosa as a basic organism, and constructing an integrated artificial hydrogen production system by using a biological enzyme-hydrogenase in the chlorella body through an external conductive medium pyrrole and an electronsupply system. The method enables the biological enzyme to be efficiently and fully utilized, not only can maintain the activity of life body of chlorella cells, but also can continue to utilize active substances in the body of the chlorella cells after the life body is dead, so that the chlorella pyrenoidosa can continuously and efficiently produce hydrogen in an anaerobic environment for more than two months.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for maintaining continuous hydrogen production after chlorella cell death. Background technique [0002] With the increasing development of the global economy, people's demand for energy is also increasing, and the resulting environmental problems are becoming increasingly urgent. Fossil energy is becoming increasingly exhausted. It is particularly important to find a substitute for fossil fuels such as coal and oil. As a kind of renewable energy and its large-scale production, biofuel has aroused the attention and thinking of more and more scientific and technological workers. [0003] As a clean and efficient energy source, hydrogen energy is widely favored by people because of its high calorific value and high energy conversion rate. However, there are still many problems in the current method of hydrogen production. Hydrogen production by electrolysis of wat...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/89
CPCC12P3/00
Inventor 黄鑫祁家瑞刘小曼
Owner HARBIN INST OF TECH
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