Kit for determining serum amyloid protein A content with scatter turbidimetry

A serum starch and turbidimetric technology, which is applied in the fields of biotechnology and medical testing, can solve the problems of limited promotion, difficult determination of serum amyloid A concentration, inaccurate test results, etc., so as to improve the sensitivity, solve the detection sensitivity, improve the Effects of Specificity and Accuracy

Active Publication Date: 2019-08-23
山东艾科达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of this method is affected by cholesterol, resulting in inaccurate detection results, which brings difficulties to the determination of serum amyloid A concentration, thus limiting the clinical promotion of such reagents

Method used

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  • Kit for determining serum amyloid protein A content with scatter turbidimetry
  • Kit for determining serum amyloid protein A content with scatter turbidimetry
  • Kit for determining serum amyloid protein A content with scatter turbidimetry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 A method for measuring serum amyloid A in serum, plasma or whole blood using a kit

[0024] 1) Preparation of calibrator: prepare serum amyloid A calibrator antigen standards with different concentration gradients with calibrator diluents to obtain final concentrations of 0mg / L, 6.3mg / L, 12.6mg / L, 25.2mg / L , 50.3mg / L, 100.6mg / L serum amyloid A calibrator antigen standard solution.

[0025] 2) Intravenous blood collection, the whole blood sample is placed in an EDTA or heparin anticoagulant tube; the serum sample is placed in a tube without anticoagulant; the plasma sample is placed in an EDTA or heparin anticoagulant tube, and gently inverted to mix. Serum and plasma samples are centrifuged at 2500-3000 rpm for 8-10 minutes, and the upper liquid (serum or plasma) is collected to obtain samples.

[0026] 3) Use Shandong Aikeda automatic specific protein analyzer for determination, set the instrument parameters as detection temperature 37℃, detection wavelength 650nm,...

Embodiment 2

[0028] Example 2 A kit for determination of serum amyloid A containing different amounts of alfalfa saponin

[0029] 1. Methods and reagents

[0030] 1. Serum amyloid A determination kit containing different amounts of alfalfa saponin determination reagent 1

[0031] Serum amyloid A determination kit determination reagent 1-A: 0.2mol / L phosphate buffer, 0.5mol / L sodium chloride, 0.02%wt ProClin300, 0%wt alfalfa saponin.

[0032] The components of serum amyloid A determination reagent 1-B, C, D, E, F, G, H and I are the same as reagent 1-A, except for the content of alfalfa saponin, they are 0.01%wt alfalfa saponin, 0.02% wt alfalfa saponin, 0.03% wt alfalfa saponin, 0.04% wt alfalfa saponin, 0.05% wt alfalfa saponin, 0.06% wt alfalfa saponin, 0.07% wt alfalfa saponin, and 0.08% wt alfalfa saponin.

[0033] 2. Serum amyloid A determination kit determination reagent 2: 0.2mol / L phosphate buffer, 5% (w / v) anti-human serum amyloid A antibody latex, 0.5mol / L sodium chloride, 1% wt bovine s...

Embodiment 3

[0042] Example 3 Test in a kit for determination of serum amyloid A containing different glycerol concentrations

[0043] 1. Methods and reagents

[0044] 1. Serum amyloid A determination kit containing different glycerol concentration determination reagent 1

[0045] Serum amyloid A determination kit determination reagent 1: 0.2mol / L phosphate buffer, 0.5mol / L sodium chloride, 0.02%wt ProClin300.

[0046] 2. Serum amyloid A determination kit containing different concentrations of glycerol Assay reagent 2-A: 0.2mol / L phosphate buffer, 5% (w / v) anti-human serum amyloid A antibody latex, 0.5mol / L sodium chloride, 1%wt bovine serum albumin, 0.02%wt ProClin 300; glycerol 0mmol / L.

[0047] The components of serum amyloid A determination reagent 2-B, C, D, E, F, and G are the same as reagent 2-A, except that the glycerol concentration is different, they are 1mmol / L, 2mmol / L, 4mmol / L, respectively , 6mmol / L, 8mmol / L and 10mmol / L.

[0048] 3. Preparation of serum samples containing different ...

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Abstract

The invention discloses a kit for determining serum amyloid protein A content with scatter turbidimetry. The kit is simple in composition, and alfalfa saponin is added and can be combined with cholesterol to reduce total cholesterol in serum, so that the interference of cholesterol can be effectively eliminated. The kit is implemented by the scatter turbidimetry, has a larger detection linear range and higher relevance and sensitivity than the existing kit, remarkably improves the detection sensitivity of serum amyloid protein A, and solves the problems of low detection sensitivity, low precision and narrow linear range in the existing serum amyloid protein A determination kit.

Description

Technical field [0001] The invention relates to a kit for determining serum amyloid A content by a scattering turbidimetric method, which belongs to the fields of biotechnology and medical testing. Background technique [0002] Serum amyloid A protein (SAA) is a polymorphic protein family encoded by multiple genes. It belongs to the acute phase response protein, a heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12,000. During inflammation, infectious and non-infectious diseases, its concentration in the blood can rise sharply within a few hours, up to 1000 times the initial concentration, but the half-life is very short, only about 50 minutes; SAA and HDL Related, it can regulate the metabolism of HDL during inflammation. A particularly important feature of SAA is that its degradation products can be deposited in different organs in the form of amyloid A fibrils. This is a serious problem in chronic inflammatory diseases. complicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/51
CPCG01N21/51
Inventor 谭柏清齐笑马万山王醒赵志敏
Owner 山东艾科达生物科技有限公司
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